B6JOlaHsd.Cg-Tg(FUS)2Kt/H
| Status | Available to order |
| EMMA ID | EM:13155 |
| International strain name | B6JOlaHsd.Cg-Tg(FUS)2Kt/H |
| Alternative name | Tg (WT Fus) 2 Kt |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(FUS)2Kt |
| Gene/Transgene symbol | Tg(FUS)2Kt |
Information from provider
| Provider | Kevin Talbot |
| Provider affiliation | Nuffield Department of Clinical Neuroscience, University of Oxford |
| Genetic information | Two BAC constructs containing genomic wild-type and P525L FUS (fused in sarcoma) were generated by subcloning the human genomic FUS locus from a 225 kb BAC, RP11-388M20 (BACPAC resource centre, Children’s Hospital Oakland Research Institute), to a pCYPAC2 backbone. The pCYPAC2 backbone was then swapped for pBACe3.6, and loxP sites and a mCherry tag were introduced. The exon 15 mutation (c. 1574C-T) was inserted separately by site-directed mutagenesis. |
| Phenotypic information | Homozygous:Not knownHeterozygous:This particular strain (BAC-FUS-WT mice, EMMA strain ID EM:13155) has no known phenotypic changes relative to non-transgenic controls while BAC-FUS-P525L mice (EMMA strain ID EM:13158) have an early onset hyperactive phenotype on open field testing. FUS-P525L-mCherry is mislocalized to the cytoplasm in primary motor neurons in vitro and in spinal cord tissue. |
| Breeding history | Founder mice (C57BL/6 x CBA)F1 were backcrossed to ~98% C57BL/6JOlaHsd (Harlan) and maintained on this background. |
| References | None available |
| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Amyotrophic lateral sclerosis / Orphanet_803
- Juvenile amyotrophic lateral sclerosis / Orphanet_300605