B6.129S1-Adgre1tm1Stst/H
| Status | Available to order |
| EMMA ID | EM:13139 |
| Citation information | RRID:IMSR_EM:13139 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129S1-Adgre1tm1Stst/H |
| Alternative name | Emr1 knockout |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Adgre1tm1Stst |
| Gene/Transgene symbol | Adgre1 |
Information from provider
| Provider | Hsi-Hsien Lin |
| Provider affiliation | Department of Microbiology and Immunology, College of Medicine, Chang Gung University |
| Additional owner | Prof. Siamon Gordon, Department of Pathology, University of Oxford, United Kingdom |
| Genetic information | The murine Adgre1 (F4/80 or Emr1) gene is located on the distal region of Chromosome 17. An F4/80-targeting construct was prepared by replacing an 1.0-kb HindIII–XbaI fragment containing the coding sequence of the first exon and the 5' end of the first intron with a promoterless-galactosidase/pGK-Neo cassette. The homologous regions in both arms of the targeting construct are a 1.8-kb fragment 5' to the first exon and a 2.8-kb fragment containing the exon 2 and the 3' end of intron 1. |
| Phenotypic information | Homozygous:The mouse macrophage-restricted ADGRE1 (F4/80) protein is not required for the development and distribution of tissue macrophages but is involved in the generation of antigen-specific efferent regulatory T (Treg) cells that suppress antigen-specific immunity. In the in vivo anterior chamber (a.c.)-associated immune deviation (ACAID) model of peripheral tolerance, a.c. inoculation of antigen into F4/80-/- mice was unable to induce efferent Treg cells and suppress delayed-type hypersensitivity (DTH) responses. Moreover, the use of anti-F4/80 mAb and F4/80-/- APCs in an in vitro ACAID model showed that all antigen presenting cells (APCs) in the culture must be able to express the F4/80 protein if efferent Treg cells were to be generated. In a low-dose oral tolerance model, wild-type but not F4/80-/- mice generated an efferent CD8+ Treg cell population that suppressed an antigen-specific DTH response. Peripheral tolerance was restored in F4/80-/- mice by adoptive transfer of F4/80+ APCs in both peripheral tolerance models, indicating a central role for the F4/80 molecule in the generation of efferent CD8+ Treg cells. In conclusion, the macrophage F4/80 receptor is required for the induction of antigen-specific efferent regulatory T cells in peripheral tolerance.Heterozygous:Same as wild-type. |
| Breeding history | Adgre1 (Emr1) knock-out mice were backcrossed to a C57BL/6J line for 10 generations and the established homozygous line has been maintained as brother x sister mate within the Univ. of Oxford facility. They breed just as good as the background strain with average litter sizes of 5/7 pups and the health status has remained consistent for many years. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | yes |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- The macrophage F4/80 receptor is required for the induction of antigen-specific efferent regulatory T cells in peripheral tolerance.;Lin Hsi-Hsien, Faunce Douglas E, Stacey Martin, Terajewicz Ania, Nakamura Takahiko, Zhang-Hoover Jie, Kerley Marilyn, Mucenski Michael L, Gordon Siamon, Stein-Streilein Joan, ;2005;The Journal of experimental medicine;201;1615-25; 15883173