C57BL/6-Tg(Ccl19-tTA,-tdTomato)687Biat/Biat
| Status | Available to order |
| EMMA ID | EM:13137 |
| International strain name | C57BL/6-Tg(Ccl19-tTA,-tdTomato)687Biat/Biat |
| Alternative name | Tg(Ccl19-tTA,-tdTomato)687Biat |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Ccl19-tTA,-tdTomato)687Biat |
| Gene/Transgene symbol | Tg(Ccl19-tTA,-tdTomato)687Biat |
Information from provider
| Provider | Burkhard Ludewig |
| Provider affiliation | Institute for Immunobiology, Kantosspital St.Gallen |
| Additional owner | Prof. Thomas Rülicke, University of Veterinary Medicine, Vienna, Austria |
| Genetic information | Mice were generated by bacterial artificial chromosome transgenesis (BAC) RPCI446D10. The tetracycline transactivator gene (tTA) was inserted downstream of the Ccl19 promoter and followed by an internal ribosomal entry site (IRES) with downstream tdTomato gene and polyadenylation signal. The recombined BAC construct obtained was microinjected into oocytes from C57BL/6 mice. |
| Phenotypic information | Homozygous:Ccl19-expressing cells express tTA and tdTomato.Heterozygous:Ccl19-expressing cells express tTA and tdTomato. Similar to EMMA strain EM:12720. However in this strain the Ccl19-expressing cells express tTA and tdTomato at a lower level compared to EM:12720. Consequently tdTomato was not detected in histology sections of lymphnodes or by flow cytometry. When crossed to Tg(tetO-cre)LC1Bjd and the Gt(ROSA)26Sor-StopFlox-EYFP reporter strain, EYFP- positive cells can be identified in lymphoid tissues. |
| Breeding history | Mice were backcrossed to C57BL/6 for more than 10 generations. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Veterinary Medicine, Vienna, Austria |
Literature references
- Origin and differentiation trajectories of fibroblastic reticular cells in the splenic white pulp.;Cheng Hung-Wei, Onder Lucas, Novkovic Mario, Soneson Charlotte, Lütge Mechthild, Pikor Natalia, Scandella Elke, Robinson Mark D, Miyazaki Jun-Ichi, Tersteegen Anne, Sorg Ursula, Pfeffer Klaus, Rülicke Thomas, Hehlgans Thomas, Ludewig Burkhard, ;2019;Nature communications;10;1739; 30988302
- Visualization of T Cell Migration in the Spleen Reveals a Network of Perivascular Pathways that Guide Entry into T Zones.;Chauveau Anne, Pirgova Gabriela, Cheng Hung-Wei, De Martin Angelina, Zhou Felix Y, Wideman Sarah, Rittscher Jens, Ludewig Burkhard, Arnon Tal I, ;2020;Immunity;52;794-807.e7; 32298648