C57BL/6-Tg(Ccl19-tTA,-tdTomato)687Biat/Biat

Status

Available to order

EMMA IDEM:13137
International strain nameC57BL/6-Tg(Ccl19-tTA,-tdTomato)687Biat/Biat
Alternative nameTg(Ccl19-tTA,-tdTomato)687Biat
Strain typeTransgenic Strains
Allele/Transgene symbolTg(Ccl19-tTA,-tdTomato)687Biat
Gene/Transgene symbolTg(Ccl19-tTA,-tdTomato)687Biat

Information from provider

ProviderBurkhard Ludewig
Provider affiliationInstitute for Immunobiology, Kantosspital St.Gallen
Additional ownerProf. Thomas Rülicke, University of Veterinary Medicine, Vienna, Austria
Genetic informationMice were generated by bacterial artificial chromosome transgenesis (BAC) RPCI446D10. The tetracycline transactivator gene (tTA) was inserted downstream of the Ccl19 promoter and followed by an internal ribosomal entry site (IRES) with downstream tdTomato gene and polyadenylation signal. The recombined BAC construct obtained was microinjected into oocytes from C57BL/6 mice.
Phenotypic informationHomozygous:
Ccl19-expressing cells express tTA and tdTomato.

Heterozygous:
Ccl19-expressing cells express tTA and tdTomato. Similar to EMMA strain EM:12720. However in this strain the Ccl19-expressing cells express tTA and tdTomato at a lower level compared to EM:12720. Consequently tdTomato was not detected in histology sections of lymphnodes or by flow cytometry. When crossed to Tg(tetO-cre)LC1Bjd and the Gt(ROSA)26Sor-StopFlox-EYFP reporter strain, EYFP- positive cells can be identified in lymphoid tissues.
Breeding historyMice were backcrossed to C57BL/6 for more than 10 generations.
References
  • Origin and differentiation trajectories of fibroblastic reticular cells in the splenic white pulp.;Cheng Hung-Wei, Onder Lucas, Novkovic Mario, Soneson Charlotte, Lütge Mechthild, Pikor Natalia, Scandella Elke, Robinson Mark D, Miyazaki Jun-Ichi, Tersteegen Anne, Sorg Ursula, Pfeffer Klaus, Rülicke Thomas, Hehlgans Thomas, Ludewig Burkhard, ;2019;Nature communications;10;1739; 30988302
  • Visualization of T Cell Migration in the Spleen Reveals a Network of Perivascular Pathways that Guide Entry into T Zones.;Chauveau Anne, Pirgova Gabriela, Cheng Hung-Wei, De Martin Angelina, Zhou Felix Y, Wideman Sarah, Rittscher Jens, Ludewig Burkhard, Arnon Tal I, ;2020;Immunity;52;794-807.e7; 32298648
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreUniversity of Veterinary Medicine, Vienna, Austria

Literature references

  • Origin and differentiation trajectories of fibroblastic reticular cells in the splenic white pulp.;Cheng Hung-Wei, Onder Lucas, Novkovic Mario, Soneson Charlotte, Lütge Mechthild, Pikor Natalia, Scandella Elke, Robinson Mark D, Miyazaki Jun-Ichi, Tersteegen Anne, Sorg Ursula, Pfeffer Klaus, Rülicke Thomas, Hehlgans Thomas, Ludewig Burkhard, ;2019;Nature communications;10;1739; 30988302
  • Visualization of T Cell Migration in the Spleen Reveals a Network of Perivascular Pathways that Guide Entry into T Zones.;Chauveau Anne, Pirgova Gabriela, Cheng Hung-Wei, De Martin Angelina, Zhou Felix Y, Wideman Sarah, Rittscher Jens, Ludewig Burkhard, Arnon Tal I, ;2020;Immunity;52;794-807.e7; 32298648

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
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Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

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