IMPC phenotypes (gene matching)
- preweaning lethality, incomplete penetrance / IMPC
B6.Cg-Loxl2tm1.2Acan/Cnbc
| Status | Available to order |
| EMMA ID | EM:13123 |
| International strain name | B6.Cg-Loxl2tm1.2Acan/Cnbc |
| Alternative name | CFBbl6 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Loxl2tm1.2Acan |
| Gene/Transgene symbol | Loxl2 |
Information from provider
| Provider | Francisco Portillo |
| Provider affiliation | Biochemistry, Universidad Autónoma de Madrid |
| Additional owner | Amparo Cano and Alberto Martin, Universidad Autónoma de Madrid, Madrid, Spain |
| Genetic information | Loxl2 constitutive knock-out. |
| Phenotypic information | Homozygous:Lethality of homozygous mutant mice observed in 100% crosses of heterozygous mice analysed so far. This contrasts with the partial perinatal lethality observed in homozygous mutant mice in a mixed genetic background (see EMMA strains EM:12882 and EM:12916).Heterozygous:Apparently normal |
| Breeding history | Conditional knock-out mutation of Loxl2 based on cre/loxP system was introduced by homologous recombination in the endogenous locus of Loxl2. ES clones positive for the mutation were microinjected/aggregated into receptive blastocysts/morulae and chimeras obtained. These animals were crossed with cre recombinase-expressing mice to check germline transmission and, at the same time, to obtain animals harbouring a constitutive knock-out allele. The resultant heterozygous animals were intercrossed and knock-out animals obtained. Homozygous females in a mixed genetic background were intercrossed with C57BL/6J males for 12 generations to obtain animals harbouring a constitutive knock-out allele in a pure C57BL/6J background. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous C57BL/6J |
Disease and phenotype information
Literature references
- Lysyl oxidase-like 2 represses Notch1 expression in the skin to promote squamous cell carcinoma progression.;Martin Alberto, Salvador Fernando, Moreno-Bueno Gema, Floristán Alfredo, Ruiz-Herguido Cristina, Cuevas Eva P, Morales Saleta, Santos Vanesa, Csiszar Katalin, Dubus Pierre, Haigh Jody J, Bigas Anna, Portillo Francisco, Cano Amparo, ;2015;The EMBO journal;34;1090-109; 25759215