C57BL/6-Slc20a2^{tm1c(EUCOMM)Wtsi}/Orl

Status	Available to order
EMMA ID	EM:13085
International strain name	C57BL/6-Slc20a2^{tm1c(EUCOMM)Wtsi}/Orl
Alternative name	Slc20a2-tm1Nhch
Strain type	Targeted Mutant Strains : Conditional mutation
Allele/Transgene symbol	Slc20a2^{tm1c(EUCOMM)Wtsi}
Gene/Transgene symbol	Slc20a2

Information from provider

Provider	Nati Hernando
Provider affiliation	Physiology, University Zurich
Additional owner	Dr. Carsten Wagner, Institute of Physiology, University Zurich, CH
Genetic information	Embryonic stem (ES) cells transfected with a Slc20a2 targeting vector were purchased from the International Knockout Mouse Consortium (IKMC)/ European Conditional Mouse Mutagenesis Program (EUCOMM). In these ES cells, the exon 4 of the sodium/phosphate cotransporter Slc20a2/Pit-2 was flanked by loxP sites. Upstream of the 5’ loxP site, the vector contained a cassette for production of beta-galactosidase, followed by a neomycin phosphotransferase gene cassette. ES cells were sent to the Institute of Labortierkunde (LTK) of the University of Zurich for morula aggregation and implantation into foster mothers. Upon breeding to homozygosity, floxed/floxed mice were crossed with flp recombinase full deleters (64FlpB6) for removal of the beta-gal/neo cassettes.
Phenotypic information	Homozygous: An intestinal-specific knockout was generated by breeding floxed Slc20a2 tm1c mice with villin-cre mice, in which the expression of the cre recombinase is under the control of the villin promoter. No differences in phosphate homeostasis were found between wild-types and homozygous littermates (Pastor-Arroyo et al., 2020, PubMed ID: 32564464). Heterozygous: Heterozygous mice were not analysed, but they are not expected to have any phenotype since homozygous mice did not show any.
Breeding history	Mice breed without problems.
References	Intestinal epithelial ablation of Pit-2/Slc20a2 in mice leads to sustained elevation of vitamin D₃ upon dietary restriction of phosphate.;Pastor-Arroyo Eva M, Knöpfel Thomas, Imenez Silva Pedro H, Schnitzbauer Udo, Poncet Nadège, Biber Jürg, Wagner Carsten A, Hernando Nati, ;2020;Acta physiologica (Oxford, England);230;e13526; 32564464
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	yes
Immunocompromised	no

Information from EMMA

Archiving centre	Institut de Transgenose, INTRAGENE, Orléans, France

Disease and phenotype information

IMPC phenotypes (gene matching)

eyelids fail to open / IMPC
decreased total retina thickness / IMPC
cataract / IMPC
abnormal incisor color / IMPC
abnormal lens morphology / IMPC
decreased bone mineral density / IMPC
decreased bone mineral content / IMPC
impaired glucose tolerance / IMPC
abnormal vitreous body morphology / IMPC
increased circulating magnesium level / IMPC
developmental and structural abnormality / IMPC
increased total body fat amount / IMPC
developmental dysplasia / IMPC
decreased body length / IMPC
narrow eye opening / IMPC
preweaning lethality, incomplete penetrance / IMPC
abnormal vocalization / IMPC
abnormal spine curvature / IMPC
increased circulating alkaline phosphatase level / IMPC
abnormal retina vasculature morphology / IMPC
mineralisation / IMPC
irregularly shaped pupil / IMPC
increased grip strength / IMPC
kyphosis / IMPC
absent pinna reflex / IMPC
abnormal retina morphology / IMPC
abnormal auditory brainstem response / IMPC
abnormal iris morphology / IMPC
abnormal motor coordination/balance / IMPC

MGI phenotypes (gene matching)

abnormal mineral level / MGI
decreased circulating phosphate level / MGI
abnormal atrioventricular cushion morphology / MGI
abnormal forebrain morphology / MGI
abnormal cerebral cortex morphology / MGI
abnormal thalamus morphology / MGI
small superior cervical ganglion / MGI
ectopic cartilage / MGI
fusion of vertebral arches / MGI
abnormal basal ganglion morphology / MGI
abnormal vena cava morphology / MGI
arteriovenous malformation / MGI
microgliosis / MGI
abnormal liver vasculature morphology / MGI
anomalous pulmonary venous connection / MGI
abnormal hypoglossal nerve topology / MGI
subcutaneous edema / MGI
multiple persisting craniopharyngeal ducts / MGI
embryo tumor / MGI
embryo cyst / MGI
hemangioma / MGI
calcified brain / MGI

Literature references

Intestinal epithelial ablation of Pit-2/Slc20a2 in mice leads to sustained elevation of vitamin D₃ upon dietary restriction of phosphate.;Pastor-Arroyo Eva M, Knöpfel Thomas, Imenez Silva Pedro H, Schnitzbauer Udo, Poncet Nadège, Biber Jürg, Wagner Carsten A, Hernando Nati, ;2020;Acta physiologica (Oxford, England);230;e13526; 32564464

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6-Slc20a2tm1c(EUCOMM)Wtsi/Orl