IMPC phenotypes (gene matching)
B6.129P2-Trp73tm1Mak/H
| Status | Available to order |
| EMMA ID | EM:13070 |
| International strain name | B6.129P2-Trp73tm1Mak/H |
| Alternative name | TAp73-/- |
| Strain type | Targeted Mutant Strains : Other targeted |
| Allele/Transgene symbol | Trp73tm1Mak |
| Gene/Transgene symbol | Trp73 |
Information from provider
| Provider | Gerry Melino |
| Provider affiliation | MRC Toxicology Unit |
| Additional owner | Prof. Tak Mak, University of Toronto, Canada |
| Genetic information | Mutant mice deficient for Trp73 exons 2 and 3, which are specific for the TAp73 isoforms, were generated by conventional gene targeting procedures in 129/SvOla embryonic stem cells. Blastocysts were transferred to pseudopregnant C57BL/6J female mice. TAp73 wild-type (+/+; TAp73), heterozygous (+/-; TAp73+/-), and homozygous (-/-; TAp73) littermate mice (129/SvOla × C57BL/6J) were generated by intercrossing TAp73+/- mice (Genes Dev., 2008 Oct 1;22(19):2677-91. doi: 10.1101/gad.1695308). |
| Phenotypic information | Homozygous:A percentage of TAp73-/- mice die during embryogenesis, resulting in an abnormal Mendelian ratio at birth (+/+, 29%; +/-, 52%; -/- 19%). Unable to obtain pups from mating trials involving mating a TAp73-/- male or female with wild-type mice. No oocytes were present in the fallopian tubes of TAp73-/- females 16 h after administration of hCG. The ovulated oocytes were trapped under the bursa and did not progress toward the fallopian tubes. TAp73-/- mice show hippocampal dysgenesis; the lower blade of the dentate gyrus was either completely missing or greatly truncated. 73% of TAp73 mice of a mixed genetic background spontaneously developed malignancies (Fig. 5B,C in Genes Dev., 2008;22(19):2677-91). Lung adenocarcinoma was the most frequent cancer that developed in TAp73 -/-mice, being observed in 32% of all TAp73 mice analysed and representing 44% of all tumours excised from TAp73 -/- mice (Genes Dev., 2008 Oct 1;22(19):2677-91. doi: 10.1101/gad.1695308). Heterozygous:No abnormal phenotype is observed in heterozygous mice (compared to wild-type littermate) |
| Breeding history | Backcrossed for more than 10 generations. |
| References |
|
| Homozygous fertile | no |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Disease and phenotype information
MGI phenotypes (gene matching)
- absent Cajal-Retzius cell / MGI
- domed cranium / MGI
- gastrointestinal hemorrhage / MGI
- absent enterocytes / MGI
- excessive digestive secretion / MGI
- abnormal cerebral cortex morphology / MGI
- abnormal cortical marginal zone morphology / MGI
- abnormal hippocampus morphology / MGI
- abnormal hippocampus development / MGI
- abnormal dentate gyrus morphology / MGI
- abnormal choroid plexus morphology / MGI
- dilated lateral ventricles / MGI
- abnormal hypothalamus morphology / MGI
- abnormal trigeminal ganglion morphology / MGI
- decreased body size / MGI
- submission towards male mice / MGI
- reduced male mating frequency / MGI
- abnormal embryo development / MGI
- periorbital edema / MGI
- increased susceptibility to otitis media / MGI
- conjunctivitis / MGI
- rhinitis / MGI
- sinus inflammation / MGI
- hydroencephaly / MGI
- non-obstructive hydrocephaly / MGI
- abnormal cerebrospinal fluid production / MGI
- intracranial hemorrhage / MGI
- intraventricular hemorrhage / MGI
- reduced fertility / MGI
- male infertility / MGI
- female infertility / MGI
- abnormal ovulation / MGI
- neoplasm / MGI
- increased cellular sensitivity to gamma-irradiation / MGI
- increased malignant tumor incidence / MGI
- increased tumor incidence / MGI
- increased T cell derived lymphoma incidence / MGI
- increased lung adenocarcinoma incidence / MGI
- increased carcinoma incidence / MGI
- increased lung adenoma incidence / MGI
- premature death / MGI
- neurodegeneration / MGI
- abnormal vomeronasal organ morphology / MGI
- increased susceptibility to bacterial infection / MGI
- chronic inflammation / MGI
- abnormal hippocampal mossy fiber morphology / MGI
- abnormal cell cycle / MGI
- increased neuron apoptosis / MGI
- abnormal forebrain development / MGI
- increased hemangiosarcoma incidence / MGI
- maternal effect / MGI
- abnormal chromosome number / MGI
- polyploidy / MGI
- increased incidence of tumors by chemical induction / MGI
- cachexia / MGI
- abnormal female meiosis / MGI
- decreased oocyte number / MGI
- decreased Cajal-Retzius cell number / MGI
- abnormal dendrite morphology / MGI
- abnormal hippocampus CA3 region morphology / MGI
- anovulation / MGI
- abnormal ovarian follicle number / MGI
- increased physiological sensitivity to xenobiotic / MGI
- increased sensitivity to induced cell death / MGI
- decreased neuron number / MGI
- distended ileum / MGI
- increased thymocyte apoptosis / MGI
- abnormal meiotic spindle morphology / MGI
- abnormal hippocampus neuron morphology / MGI
- postnatal lethality, incomplete penetrance / MGI
- prenatal lethality, incomplete penetrance / MGI
- increased lymphoma incidence / MGI
Literature references
- TAp73 knockout shows genomic instability with infertility and tumor suppressor functions.;Tomasini Richard, Tsuchihara Katsuya, Wilhelm Margareta, Fujitani Masashi, Rufini Alessandro, Cheung Carol C, Khan Fatima, Itie-Youten Annick, Wakeham Andrew, Tsao Ming-Sound, Iovanna Juan L, Squire Jeremy, Jurisica Igor, Kaplan David, Melino Gerry, Jurisicova Andrea, Mak Tak W, ;2008;Genes & development;22;2677-91; 18805989