IMPC phenotypes (gene matching)
B6.Cg-Trp73tm1.1Gml/H
| Status | Available to order |
| EMMA ID | EM:13068 |
| International strain name | B6.Cg-Trp73tm1.1Gml/H |
| Alternative name | Trp73D13/D13 (Trp73delta13) |
| Strain type | Targeted Mutant Strains : Other targeted |
| Allele/Transgene symbol | targeted mutation 1.1, Gerry Melino |
| Gene/Transgene symbol | Trp73 |
Information from provider
| Provider | Gerry Melino |
| Provider affiliation | MRC Toxicology Unit |
| Genetic information | Trp73D13/D13 mice were generated using the cre-loxP system. First, floxed (Trp73fl/fl) mice (EMMA strain EM:13067) were obtained by introducing a vector containing loxP sites flanking exon 13 of the Trp73 gene, which was replaced by a neoR cassette to enable selection. Immediately upstream and downstream of these sites, long terminal repeats (LTRs) facilitated incorporation of the vector into ES cells by homologous recombination. Mice where then crossed with Flp recombinase expressing animals to remove the NeoR cassette. Floxed mice were subsequently crossed with mice ubiquitously expressing cre recombinase under the human cytomegalovirus promoter (CMV-cre) to delete exon 13 in all tissues. |
| Phenotypic information | Homozygous:Trp73D13/D13 mice are significantly smaller than control mice beginning at P7, and 40% of them die within the first 3 weeks of life. The hippocampal architecture of Trp73D13/D13 mice is completely misshapen. Hippocampal neurons are able to form CA and DG cell layers, but these regions are morphologically misassembled and displayed a severely altered architecture. The observed hippocampal dysgenesis strongly impairs memory and learning, and it determines a reduction of synaptic transmission. Trp73D13/D13 also show an impaired adult neurogenesis process. Furthermore, a massive loss of Cajal-Retzius cells is observed in the Trp73D13/D13 mice.Heterozygous:No abnormal phenotype is observed in heterozygous mice (compared to wild-type littermates). |
| Breeding history | Backcrossed more than 10 generations. |
| References |
|
| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Disease and phenotype information
MGI phenotypes (gene matching)
- absent Cajal-Retzius cell / MGI
- domed cranium / MGI
- gastrointestinal hemorrhage / MGI
- absent enterocytes / MGI
- excessive digestive secretion / MGI
- abnormal cerebral cortex morphology / MGI
- abnormal cortical marginal zone morphology / MGI
- abnormal hippocampus morphology / MGI
- abnormal hippocampus development / MGI
- abnormal dentate gyrus morphology / MGI
- abnormal choroid plexus morphology / MGI
- dilated lateral ventricles / MGI
- abnormal hypothalamus morphology / MGI
- abnormal trigeminal ganglion morphology / MGI
- decreased body size / MGI
- submission towards male mice / MGI
- reduced male mating frequency / MGI
- abnormal embryo development / MGI
- periorbital edema / MGI
- increased susceptibility to otitis media / MGI
- conjunctivitis / MGI
- rhinitis / MGI
- sinus inflammation / MGI
- hydroencephaly / MGI
- non-obstructive hydrocephaly / MGI
- abnormal cerebrospinal fluid production / MGI
- intracranial hemorrhage / MGI
- intraventricular hemorrhage / MGI
- reduced fertility / MGI
- male infertility / MGI
- female infertility / MGI
- abnormal ovulation / MGI
- neoplasm / MGI
- increased cellular sensitivity to gamma-irradiation / MGI
- increased malignant tumor incidence / MGI
- increased tumor incidence / MGI
- increased T cell derived lymphoma incidence / MGI
- increased lung adenocarcinoma incidence / MGI
- increased carcinoma incidence / MGI
- increased lung adenoma incidence / MGI
- premature death / MGI
- neurodegeneration / MGI
- abnormal vomeronasal organ morphology / MGI
- increased susceptibility to bacterial infection / MGI
- chronic inflammation / MGI
- abnormal hippocampal mossy fiber morphology / MGI
- abnormal cell cycle / MGI
- increased neuron apoptosis / MGI
- abnormal forebrain development / MGI
- increased hemangiosarcoma incidence / MGI
- maternal effect / MGI
- abnormal chromosome number / MGI
- polyploidy / MGI
- increased incidence of tumors by chemical induction / MGI
- cachexia / MGI
- abnormal female meiosis / MGI
- decreased oocyte number / MGI
- decreased Cajal-Retzius cell number / MGI
- abnormal dendrite morphology / MGI
- abnormal hippocampus CA3 region morphology / MGI
- anovulation / MGI
- abnormal ovarian follicle number / MGI
- increased physiological sensitivity to xenobiotic / MGI
- increased sensitivity to induced cell death / MGI
- decreased neuron number / MGI
- distended ileum / MGI
- increased thymocyte apoptosis / MGI
- abnormal meiotic spindle morphology / MGI
- abnormal hippocampus neuron morphology / MGI
- postnatal lethality, incomplete penetrance / MGI
- prenatal lethality, incomplete penetrance / MGI
- increased lymphoma incidence / MGI
Literature references
- The C terminus of p73 is essential for hippocampal development.;Amelio Ivano, Panatta Emanuele, Niklison-Chirou Maria Victoria, Steinert Joern R, Agostini Massimiliano, Morone Nobuhiro, Knight Richard A, Melino Gerry, ;2020;Proceedings of the National Academy of Sciences of the United States of America;117;15694-15701; 32571922
- P73 C-terminus is dispensable for multiciliogenesis.;Buckley Niall, Panatta Emanuele, Morone Nobuhiro, Noguchi Masafumi, Scorrano Luca, Knight Richard A, Amelio Ivano, Melino Gerry, ;2020;Cell cycle (Georgetown, Tex.);19;1833-1845; 32584647