C57BL/6NCrl-Marchf3tm1b(EUCOMM)Hmgu/Ph

Status

Available to order

EMMA IDEM:12830
International strain nameC57BL/6NCrl-Marchf3tm1b(EUCOMM)Hmgu/Ph
Alternative nameHEPD0511_2_B11
Strain typeTargeted Mutant Strains
Allele/Transgene symbolMarchf3tm1b(EUCOMM)Hmgu
Gene/Transgene symbolMarchf3
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Institute of Molecular Genetics
Provider affiliationDepartment of Transgenic Models of Diseases, Institute of Molecular Genetics
Genetic informationThis mouse line originates from EUCOMM ES clone HEPD0511_2_B11. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele (Gt(ROSA)26Sortm1(ACTB-cre,-EGFP)Ics (MGI:5285392)). Click here for more information on EUCOMM final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreInstitute of Molecular Genetics, Prague, Czech Republic
Animals used for archivingheterozygous C57BL/6NCrl

Disease and phenotype information

IMPC phenotypes (allele matching)
  • abnormal heart morphology / IMPC
  • enlarged heart / IMPC
  • abnormal spleen morphology / IMPC
  • enlarged spleen / IMPC
  • abnormal thymus morphology / IMPC
  • enlarged thymus / IMPC
  • abnormal uterus morphology / IMPC
  • abnormal skin morphology / IMPC
  • abnormal kidney morphology / IMPC
  • enlarged kidney / IMPC
  • enlarged uterus / IMPC
  • enhanced contextual conditioning behavior / IMPC
  • increased fasting circulating glucose level / IMPC
IMPC phenotypes (gene matching)
  • enhanced contextual conditioning behavior / IMPC
  • abnormal kidney morphology / IMPC
  • abnormal thymus morphology / IMPC
  • enlarged kidney / IMPC
  • abnormal uterus morphology / IMPC
  • enlarged spleen / IMPC
  • abnormal heart morphology / IMPC
  • abnormal skin morphology / IMPC
  • enlarged thymus / IMPC
  • increased fasting circulating glucose level / IMPC
  • enlarged uterus / IMPC
  • abnormal spleen morphology / IMPC
  • enlarged heart / IMPC

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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