MGI phenotypes (gene matching)
B6.129-Plxnb1tm1.1Ltam/Cnrm
| Status | Available to order |
| EMMA ID | EM:12800 |
| International strain name | B6.129-Plxnb1tm1.1Ltam/Cnrm |
| Alternative name | PlxnB1-KO |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Plxnb1tm1.1Ltam |
| Gene/Transgene symbol | Plxnb1 |
Information from provider
| Provider | Luca Tamagnone |
| Provider affiliation | Istituto di Istologia ed Embriologia, Università Cattolica del Sacro Cuore |
| Genetic information | Plexin-B1 gene inactivation by insertion of loxP sites, and subsequent deletion of exons 22-23. |
| Phenotypic information | Homozygous:They do not show any major developmental phenotype, apart from a kidney phenotype appearing at E13.5 (kidneys are larger and exhibit more ureteric branches than in heterozygous controls; Perala et al., 2011). Due to important role of plexin-B1 signaling reported in the immune system and cancer microenvironment, further studies in the adult stage of this line are warranted. Especially, the knock-out model might require appropriate challenging, or the strain should be crossed with other genetically engineered mouse models with cancer or immune deficiency-related phenotypes. Note that, so far, studies in cancer have been done with a single transplanted tumor model: B16 melanoma cells (see Fazzari et al., 2007). The immunological phenotype has not been studied in these mice so far.Heterozygous:The heterozygous mice do not show any phenotype. |
| Breeding history | Chimeric mice were initially generated on 129 x C57BL/6 background, and then subjected to several generations of backcrossing. The strain is commonly bred in homozygous state. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
Literature references
- Plexin-B1 plays a redundant role during mouse development and in tumour angiogenesis.;Fazzari Pietro, Penachioni Junia, Gianola Sara, Rossi Ferdinando, Eickholt Britta J, Maina Flavio, Alexopoulou Lena, Sottile Antonino, Comoglio Paolo Maria, Flavell Richard A, Tamagnone Luca, ;2007;BMC developmental biology;7;55; 17519029
- Sema4C-Plexin B2 signalling modulates ureteric branching in developing kidney.;Perälä Nina, Jakobson Madis, Ola Roxana, Fazzari Pietro, Penachioni Junia Y, Nymark Mariann, Tanninen Tiina, Immonen Tiina, Tamagnone Luca, Sariola Hannu, ;2011;Differentiation; research in biological diversity;81;81-91; 21035938