C57BL/6-Tg(Chrna2-cre)1Kldr/Kctt
| Status | Available to order |
| EMMA ID | EM:12708 |
| Citation information | RRID:IMSR_EM:12708 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6-Tg(Chrna2-cre)1Kldr/Kctt |
| Alternative name | Chrna2-cre |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Chrna2-cre)1Kldr |
| Gene/Transgene symbol | Tg(Chrna2-cre)1Kldr |
Information from provider
| Provider | Klas Kullander |
| Provider affiliation | Neuroscience, Uppsala University |
| Genetic information | In this mouse line cre recombinase is expressed under the control of the Chrna2 promoter. A construct (composed of cre recombinase with a nuclear localization signal, SV40 polyadenylation sequence, and an Frt-kanamycin/neo-Frt cassette) was introduced into the ATG site in exon 2 of the Chrna2 gene in a bacterial artificial chromosome (BAC RP23-48P22) by recombining in bacterial cells. Modified BAC DNA was purified and injected into mouse pronuclei to produce transgenic mice. |
| Phenotypic information | Homozygous:Mice homozygous for the Chrna2-cre transgene show no clinical abnormalities. Homozygous mice are expected to be fertile, but the generation of homozygous mice has not been attempted to date.Heterozygous:Mice heterozygous for the Chrna2-cre transgene show no clinical abnormalities, they are viable and fertile. |
| Breeding history | Chrna2-cre mice have been bred for over 20 generations with C57BL/6JTac mice. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Karolinska Institutet, Stockholm, Sweden |
| Animals used for archiving | heterozygous C57BL/6J males |
Literature references
- Ventral hippocampal OLM cells control type 2 theta oscillations and response to predator odor.;Mikulovic Sanja, Restrepo Carlos Ernesto, Siwani Samer, Bauer Pavol, Pupe Stefano, Tort Adriano B L, Kullander Klas, Leão Richardson N, ;2018;Nature communications;9;3638; 30194386
- Chrna2-Martinotti Cells Synchronize Layer 5 Type A Pyramidal Cells via Rebound Excitation.;Hilscher Markus M, Leão Richardson N, Edwards Steven J, Leão Katarina E, Kullander Klas, ;2017;PLoS biology;15;e2001392; 28182735
- Novel markers for OLM interneurons in the hippocampus.;Mikulovic Sanja, Restrepo C Ernesto, Hilscher Markus M, Kullander Klas, Leão Richardson N, ;2015;Frontiers in cellular neuroscience;9;201; 26082683
- Firing properties of Renshaw cells defined by Chrna2 are modulated by hyperpolarizing and small conductance ion currents Ih and ISK.;Perry Sharn, Gezelius Henrik, Larhammar Martin, Hilscher Markus M, Lamotte d'Incamps Boris, Leao Katarina E, Kullander Klas, ;2015;The European journal of neuroscience;41;889-900; 25712471
- OLM interneurons differentially modulate CA3 and entorhinal inputs to hippocampal CA1 neurons.;Leão Richardson N, Mikulovic Sanja, Leão Katarina E, Munguba Hermany, Gezelius Henrik, Enjin Anders, Patra Kalicharan, Eriksson Anders, Loew Leslie M, Tort Adriano B L, Kullander Klas, ;2012;Nature neuroscience;15;1524-30; 23042082
- Developmental Disruption of Recurrent Inhibitory Feedback Results in Compensatory Adaptation in the Renshaw Cell-Motor Neuron Circuit.;Enjin Anders, Perry Sharn, Hilscher Markus M, Nagaraja Chetan, Larhammar Martin, Gezelius Henrik, Eriksson Anders, Leão Katarina E, Kullander Klas, ;2017;The Journal of neuroscience : the official journal of the Society for Neuroscience;37;5634-5647; 28483975
- OLMα2 Cells Bidirectionally Modulate Learning.;Siwani Samer, França Arthur S C, Mikulovic Sanja, Reis Amilcar, Hilscher Markus M, Edwards Steven J, Leão Richardson N, Tort Adriano B L, Kullander Klas, ;2018;Neuron;99;404-412.e3; 29983324