C57BL/6-Tg(Chrna2-cre)1Kldr/Kctt
| Status | Available to order |
| EMMA ID | EM:12708 |
| International strain name | C57BL/6-Tg(Chrna2-cre)1Kldr/Kctt |
| Alternative name | Chrna2-cre |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Chrna2-cre)1Kldr |
| Gene/Transgene symbol | Tg(Chrna2-cre)1Kldr |
Information from provider
| Provider | Klas Kullander |
| Provider affiliation | Neuroscience, Uppsala University |
| Genetic information | In this mouse line cre recombinase is expressed under the control of the Chrna2 promoter. A construct (composed of cre recombinase with a nuclear localization signal, SV40 polyadenylation sequence, and an Frt-kanamycin/neo-Frt cassette) was introduced into the ATG site in exon 2 of the Chrna2 gene in a bacterial artificial chromosome (BAC RP23-48P22) by recombining in bacterial cells. Modified BAC DNA was purified and injected into mouse pronuclei to produce transgenic mice. |
| Phenotypic information | Homozygous:Homozygous mice for the Chrna2-cre transgene show no clinical abnormalities. Homozygous mice are expected to be fertile, but the generation of homozygous mice has not been attempted to date.Heterozygous:Heterozygous mice for the Chrna2-cre transgene show no clinical abnormalities, they are viable and fertile. |
| Breeding history | Chrna2-cre mice have been bred for over 20 generations with C57BL/6JTac mice. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Karolinska Institutet, Stockholm, Sweden |
| Animals used for archiving | heterozygous C57BL/6J |
Literature references
- Ventral hippocampal OLM cells control type 2 theta oscillations and response to predator odor.;Mikulovic Sanja, Restrepo Carlos Ernesto, Siwani Samer, Bauer Pavol, Pupe Stefano, Tort Adriano B L, Kullander Klas, Leão Richardson N, ;2018;Nature communications;9;3638; 30194386
- Chrna2-Martinotti Cells Synchronize Layer 5 Type A Pyramidal Cells via Rebound Excitation.;Hilscher Markus M, Leão Richardson N, Edwards Steven J, Leão Katarina E, Kullander Klas, ;2017;PLoS biology;15;e2001392; 28182735
- Novel markers for OLM interneurons in the hippocampus.;Mikulovic Sanja, Restrepo C Ernesto, Hilscher Markus M, Kullander Klas, Leão Richardson N, ;2015;Frontiers in cellular neuroscience;9;201; 26082683
- Firing properties of Renshaw cells defined by Chrna2 are modulated by hyperpolarizing and small conductance ion currents Ih and ISK.;Perry Sharn, Gezelius Henrik, Larhammar Martin, Hilscher Markus M, Lamotte d'Incamps Boris, Leao Katarina E, Kullander Klas, ;2015;The European journal of neuroscience;41;889-900; 25712471
- OLM interneurons differentially modulate CA3 and entorhinal inputs to hippocampal CA1 neurons.;Leão Richardson N, Mikulovic Sanja, Leão Katarina E, Munguba Hermany, Gezelius Henrik, Enjin Anders, Patra Kalicharan, Eriksson Anders, Loew Leslie M, Tort Adriano B L, Kullander Klas, ;2012;Nature neuroscience;15;1524-30; 23042082
- Developmental Disruption of Recurrent Inhibitory Feedback Results in Compensatory Adaptation in the Renshaw Cell-Motor Neuron Circuit.;Enjin Anders, Perry Sharn, Hilscher Markus M, Nagaraja Chetan, Larhammar Martin, Gezelius Henrik, Eriksson Anders, Leão Katarina E, Kullander Klas, ;2017;The Journal of neuroscience : the official journal of the Society for Neuroscience;37;5634-5647; 28483975
- OLMα2 Cells Bidirectionally Modulate Learning.;Siwani Samer, França Arthur S C, Mikulovic Sanja, Reis Amilcar, Hilscher Markus M, Edwards Steven J, Leão Richardson N, Tort Adriano B L, Kullander Klas, ;2018;Neuron;99;404-412.e3; 29983324