IMPC phenotypes (gene matching)
C57BL/6-Csf2rbtm1c(EUCOMM)Hmgu/Orl
| Status | Available to order |
| EMMA ID | EM:12699 |
| International strain name | C57BL/6-Csf2rbtm1c(EUCOMM)Hmgu/Orl |
| Alternative name | C57BL/6N-Csf2rbtm1c(EUCOMM)Hmgu |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Csf2rbtm1c(EUCOMM)Hmgu |
| Gene/Transgene symbol | Csf2rb |
Information from provider
| Provider | Burkhard Becher |
| Provider affiliation | Institute of experimental Immunology, University of Zurich |
| Genetic information | This mouse strain was generated in the laboratory of Dr. Burkhard Becher at the University of Zürich. The L1L2_Bact_P cassette was inserted at position 78337719 of Chromosome 15 upstream of exon 4 (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 4 at position 78339575. The construct was electroporated into C57BL/6N-Atm1Brd-derived JM8A3.N1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were bred to C57BL/6-Tg(CAG-flpe)2Arte, a strain which ubiquitously expresses the flp recombinase. Upon arrival, mice were bred to C57BL/6NJ (JAX Mice Stock No. 005304) for at least 1 generation to establish the colony. |
| Phenotypic information | Homozygous:Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any physical or behavioral abnormalities. Heterozygous:Mice that are heterozygous for this allele are viable, fertile, normal in size and do not display any physical or behavioral abnormalities. |
| Breeding history | More than 100 mice, litter size is normal compared to wild-type; all genotypes are viable and fertile |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | homozygous C57BL/6N |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Hereditary pulmonary alveolar proteinosis / Orphanet_264675
Literature references
- The Cytokine GM-CSF Drives the Inflammatory Signature of CCR2+ Monocytes and Licenses Autoimmunity.;Croxford Andrew L, Lanzinger Margit, Hartmann Felix J, Schreiner Bettina, Mair Florian, Pelczar Pawel, Clausen Björn E, Jung Steffen, Greter Melanie, Becher Burkhard, ;2015;Immunity;43;502-14; 26341401
- GM-CSF-dependent CD301b+ lung dendritic cells confer tolerance to inhaled allergens.;Wilkinson Christina L, Nakano Keiko, Grimm Sara A, Whitehead Gregory S, Arao Yukitomo, Blackshear Perry J, Karmaus Peer W, Fessler Michael B, Cook Donald N, Nakano Hideki, ;2024;Research square;0;; 38883724