STOCK Fbxw7tm1.1Axbe/H
| Status | Available to order |
| EMMA ID | EM:12632 |
| International strain name | STOCK Fbxw7tm1.1Axbe/H |
| Alternative name | Floxed-Fbxw7 mouse |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Fbxw7tm1.1Axbe |
| Gene/Transgene symbol | Fbxw7 |
Information from provider
| Provider | Cancer Research UK |
| Provider affiliation | Ximbio, Cancer Research UK |
| Genetic information | The targeting vector was composed of exons 4 and 5 of genomic Fbxw7, where exon 5 was flanked with two loxP sites, and the pPGK -neoR flanked by two FRT sites in pFlrt vector. The fragment was finally inserted into pDT vector by standard cloning techniques. The targeting construct was linearized with NotI, and the purified fragment was electroporated into embryonic stem cells, and the Fbxw7 mouse generated according to standard protocols. PCR screening and consequent Southern blotting to show a single copy of specifically targeted Fbxw7 allele in embryonic stem cells were performed. A germline-specific Flp transgenic mouse is used to remove neoR, resulting in the floxed Fbxw7 (Fbxw7 fl/fl). To show in vivo inactivation of the Fbxw7 floxed allele by cre recombinase and the resultant phenotype, the Fbxw7 fl/fl homozygous mice have been crossed with mice expressing cre recombinase under control of a general pGK promoter (pGK-cre transgenic mice). We confirmed that all resulting offspring with Fbxw7 fl/fl (C57BL/6 background) and pGK-cre alleles died in utero as previously described using conventional knockout Fbxw7 mouse (Tsunematsu et al., 2004 ) and confirmed that the deletion of exon 5 abolished the function of Fbxw7. Please see the suppl. figure 1, published at: J Exp Med. 2011 Feb 14;208(2):295-312. |
| Phenotypic information | Homozygous:No overt phenotype, cre-mediated recombination required to facilitate knockdown of Fbxw7.Heterozygous:No overt phenotype, cre-mediated recombination required to facilitate knockdown of Fbxw7. |
| Breeding history | Both homozygous and heterozygous mice are viable fertile, have a normal lifespan and are not immunocompromised. Animals are reproductively mature at 6-8 weeks of age and a decline in reproductivity can be observed from around 8 months of age. Average length of gestation is 19 days and the average litter size is 7-8 pups, of which all would be expected to survive to weaning at 21 days. The average number of litters possible in a female’s lifetime is 6-7. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- FBXW7 influences murine intestinal homeostasis and cancer, targeting Notch, Jun, and DEK for degradation.;Babaei-Jadidi Roya, Li Ningning, Saadeddin Anas, Spencer-Dene Bradley, Jandke Anett, Muhammad Belal, Ibrahim ElSayed E, Muraleedharan Ranjithmenon, Abuzinadah Mohammed, Davis Hayley, Lewis Annabelle, Watson Susan, Behrens Axel, Tomlinson Ian, Nateri Abdolrahman Shams, ;2011;The Journal of experimental medicine;208;295-312; 21282377