C57BL/6NCrl-Ltn1^{tm1b(EUCOMM)Hmgu}/Ph

Status	Available to order
EMMA ID	EM:12617
International strain name	C57BL/6NCrl-Ltn1^{tm1b(EUCOMM)Hmgu}/Ph
Alternative name	HEPD0658_5_C10
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Ltn1^{tm1b(EUCOMM)Hmgu}
Gene/Transgene symbol	Ltn1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Provider	Institute of Molecular Genetics
Provider affiliation	Department of Transgenic Models of Diseases, Institute of Molecular Genetics
Genetic information	This mouse line originates from EUCOMM ES clone HEPD0658_5_C10. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele (Gt(ROSA)26Sortm1(ACTB-cre,-EGFP)Ics (MGI:5285392)). Click here for more information on EUCOMM final vectors.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Archiving centre	Institute of Molecular Genetics, Prague, Czech Republic
Animals used for archiving	heterozygous C57BL/6NCrl

IMPC phenotypes (allele matching)

decreased body weight / IMPC
abnormal cornea morphology / IMPC
no spontaneous movement / IMPC
decreased circulating alkaline phosphatase level / IMPC
thrombocytopenia / IMPC
increased red blood cell distribution width / IMPC
increased CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
increased airway resistance / IMPC
increased lung elastance / IMPC
increased lung tissue damping / IMPC
preweaning lethality, incomplete penetrance / IMPC
increased freezing behavior / IMPC

IMPC phenotypes (gene matching)

increased lung tissue damping / IMPC
increased lung elastance / IMPC
preweaning lethality, incomplete penetrance / IMPC
abnormal cornea morphology / IMPC
increased CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
thrombocytopenia / IMPC
decreased circulating alkaline phosphatase level / IMPC
increased airway resistance / IMPC
no spontaneous movement / IMPC
increased freezing behavior / IMPC
increased red blood cell distribution width / IMPC
decreased body weight / IMPC

MGI phenotypes (gene matching)

Information from provider