B6(Cg)-Mmuttm2.1Mrb/Orl

Status

Available to order

EMMA IDEM:12576
International strain nameB6(Cg)-Mmuttm2.1Mrb/Orl
Alternative nameMut-flox (B6-Mut/Pg)
Strain typeTargeted Mutant Strains : Conditional mutation
Allele/Transgene symbolMmuttm2.1Mrb
Gene/Transgene symbolMmut

Information from provider

ProviderMatthias Baumgartner
Provider affiliationMetabolism, University Children
Genetic informationExon 3 of the gene methylmalonyl-CoA mutase (Mmut) was targeted to be flanked by loxP sites. For the generation of targeted ES cell clones, C57BL/6 derived ES cells were electroporated with a linearized EUCOMM vector (EUCOMM vector ID 47381). In the targeting vector, exon 3 is flanked by loxP sites; additionally an FRT-flanked targeting cassette was inserted into intron 2. This cassette included a neomycin resistance gene and a lacZ reporter, which consisted of splice acceptor (En2 SA), IRES and beta-gal. Homologous recombination in ES cells was verified by PCR screening, restriction enzyme digest and Southern Blot. A positive ES cell clone was injected into the blastocysts of C57BL/6, resulting in chimeric mice which were set up for breeding with Flp-deleter mice. The offspring was PCR screened for i) the presence of the remaining FRT-site in the targeted allele to identify germ line transmitters; ii) the absence of the neomycin cassette; iii) the distal loxP site; iv) and the Flp transgene.
Phenotypic informationHomozygous:
There is no phenotype without cross-breeding to cre recombinase-expressing mice. The phenotype of the resulting matings depends on the cre recombinase promoter.

Heterozygous:
No phenotype.
Breeding historyMice were kept as homozygous Mmut flox/flox, by breeding Mmut flox/flox mice with other Mmut flox/flox mice from the same line. More than 20 generations.
References
  • New in vitro model derived from brain-specific Mut-/- mice confirms cerebral ammonium accumulation in methylmalonic aciduria.;Remacle Noémie, Forny Patrick, Cudré-Cung Hong-Phuc, Gonzalez-Melo Mary, do Vale-Pereira Sónia, Henry Hugues, Teav Tony, Gallart-Ayala Hector, Braissant Olivier, Baumgartner Matthias, Ballhausen Diana, ;2018;Molecular genetics and metabolism;124;266-277; 29934063
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France
Animals used for archivinghomozygous C57BL/6

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Literature references

  • New in vitro model derived from brain-specific Mut-/- mice confirms cerebral ammonium accumulation in methylmalonic aciduria.;Remacle Noémie, Forny Patrick, Cudré-Cung Hong-Phuc, Gonzalez-Melo Mary, do Vale-Pereira Sónia, Henry Hugues, Teav Tony, Gallart-Ayala Hector, Braissant Olivier, Baumgartner Matthias, Ballhausen Diana, ;2018;Molecular genetics and metabolism;124;266-277; 29934063

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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