STOCK Cryge^t/Ieg

Status	Available to order
EMMA ID	EM:12546
International strain name	STOCK Cryge^t/Ieg
Alternative name	Cat2t
Strain type	Induced Mutant Strains : Radiation-induced
Allele/Transgene symbol	Cryge^t
Gene/Transgene symbol	Cryge

Information from provider

Provider	Jana Löster (formerly Kratochvilova)
Provider affiliation	Institut of Mammalian Genetics, Helmholtz Zentrum München
Additional owner	Prof. Dr. Jochen Graw, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Institute of Developmental Genetics, Neuherberg/Germany --- Jack Favor Helmholtz Zentrum München Institute of Human Genetics Neuherberg/Germany
Genetic information	Dominant mutation in chromosome 1, gene Cryge (C432G; Tyr144X). Disclaimer - Special restrictions: 1) Mutant development was phenotype driven. Although for some mutant lines, linkage data and the molecular characterization of the causative mutation has been published, EMMA has not genotyped the strains and can not provide genotyping protocols. 2) Some lines showed low penetrance of the phenotype. 3) Only sperm available. Rederivation service can not be offered. 4) EMMA quality control standards may not apply for these strains. 5) EMMA has not verified the breeding performance and the genetic background of the strains. The genotype/phenotype of the mice is not confirmed by INFRAFRONTIER/EMMA. We strongly recommend that the recipient confirms genotype/phenotype upon receipt.
Phenotypic information	Homozygous: Total cataract, dysplasia of lens and iris, microphthalmia. Heterozygous: Total cataract, dysplasia of lens and iris, microphthalmia.
Breeding history	Kept as homozygotes.
References	Dominant cataract and recessive specific locus mutations in offspring of X-irradiated male mice.;Graw J, Favor J, Neuhäuser-Klaus A, Ehling U H, ;1986;Mutation research;159;47-54; 3941665 Allelism tests of 15 dominant cataract mutations in mice.;Kratochvilova J, Favor J, ;1992;Genetical research;59;199-203; 1511869 Three murine cataract mutants (Cat2) are defective in different gamma-crystallin genes.;Klopp N, Favor J, Löster J, Lutz R B, Neuhäuser-Klaus A, Prescott A, Pretsch W, Quinlan R A, Sandilands A, Vrensen G F, Graw J, ;1998;Genomics;52;152-8; 9782080 Characterization of Cat-2t, a radiation-induced dominant cataract mutation in mice.;Graw J, Bors W, Gopinath P M, Merkle S, Michel C, Reitmeir P, Schäffer E, Summer K H, Wulff A, ;1990;Investigative ophthalmology & visual science;31;1353-61; 1973159 Altered Na,K-ATPase pattern in gamma-crystallin mutant mice.;Moseley Amy, Graw Jochen, Delamere Nicholas A, ;2002;Investigative ophthalmology & visual science;43;1517-9; 11980868 Mapping of six dominant cataract genes in the mouse.;Everett C A, Glenister P H, Taylor D M, Lyon M F, Kratochvilova-Loester J, Favor J, ;1994;Genomics;20;429-34; 8034315
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	not known
Immunocompromised	not known

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany

Disease and phenotype information

MGI phenotypes (gene matching)

microphthalmia / MGI
abnormal lens morphology / MGI
cataract / MGI
small lens / MGI
small pupils / MGI
abnormal iris morphology / MGI
abnormal lens fiber morphology / MGI
abnormal lens capsule morphology / MGI
abnormal lens development / MGI
ruptured lens capsule / MGI
nuclear cataracts / MGI
total cataracts / MGI
vacuolated lens / MGI
immature cataract / MGI

Literature references

Dominant cataract and recessive specific locus mutations in offspring of X-irradiated male mice.;Graw J, Favor J, Neuhäuser-Klaus A, Ehling U H, ;1986;Mutation research;159;47-54; 3941665
Allelism tests of 15 dominant cataract mutations in mice.;Kratochvilova J, Favor J, ;1992;Genetical research;59;199-203; 1511869
Three murine cataract mutants (Cat2) are defective in different gamma-crystallin genes.;Klopp N, Favor J, Löster J, Lutz R B, Neuhäuser-Klaus A, Prescott A, Pretsch W, Quinlan R A, Sandilands A, Vrensen G F, Graw J, ;1998;Genomics;52;152-8; 9782080
Characterization of Cat-2t, a radiation-induced dominant cataract mutation in mice.;Graw J, Bors W, Gopinath P M, Merkle S, Michel C, Reitmeir P, Schäffer E, Summer K H, Wulff A, ;1990;Investigative ophthalmology & visual science;31;1353-61; 1973159
Altered Na,K-ATPase pattern in gamma-crystallin mutant mice.;Moseley Amy, Graw Jochen, Delamere Nicholas A, ;2002;Investigative ophthalmology & visual science;43;1517-9; 11980868
Mapping of six dominant cataract genes in the mouse.;Everett C A, Glenister P H, Taylor D M, Lyon M F, Kratochvilova-Loester J, Favor J, ;1994;Genomics;20;429-34; 8034315

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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STOCK Cryget/Ieg