B6.C3H-Mapk10m1Mhda/Ieg

Status

Available to order

EMMA IDEM:12505
International strain nameB6.C3H-Mapk10m1Mhda/Ieg
Alternative nameMapk10-Gln100Leu
Strain typeInduced Mutant Strains : Chemically-induced
Allele/Transgene symbolMapk10m1Mhda
Gene/Transgene symbolMapk10

Information from provider

ProviderMartin Hrabé de Angelis
Provider affiliationInstitute of Experimental Genetics, Helmholtz Center Munich
Additional ownerProf. Dr. Jochen Graw, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Institute of Developmental Genetics, Neuherberg/Germany
Genetic informationMapk10, c. 299, A->T, leading to p. Gln100Leu exchange

Disclaimer - Special restrictions:

1) Mutant development was phenotype driven. Although for some mutant lines, linkage data and the molecular characterization of the causative mutation has been published, EMMA has not genotyped the strains and can not provide genotyping protocols.
2) Some lines showed low penetrance of the phenotype.
3) Only sperm available. Rederivation service can not be offered.
4) EMMA quality control standards may not apply for these strains.
5) EMMA has not verified the breeding performance and the genetic background of the strains.

The genotype/phenotype of the mice is not confirmed by INFRAFRONTIER/EMMA. We strongly recommend that the recipient confirms genotype/phenotype upon receipt.
Phenotypic informationHomozygous:
No obvious phenotype

Heterozygous:
No obvious phenotype
Breeding historyThe mutation was selected from an ENU sperm archive (Sabrautzki S, Kaiser G, Przemeck GKH, Gerst F, Lorza-Gil E, Panse M, Sartorius T, Hoene M, Marschall S, Häring H-U, Hrabe de Angelis M, Ullrich S: Point mutation of Ffar1 abrogates fatty acid-dependent insulin secretion, but protects against HFD-induced glucose intolerance. Mol Metab 6, 2017; 1304-1312). The mutation was originally on a C3HeB/FeJ background, but was outcrossed for 7 generations to C57BL/6JG. The Mapk10-mutant line is free of the rd1 mutation (causing retinal degeneration).
ReferencesNone available
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisednot known

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archivingheterozygous C57BL/6J

Disease and phenotype information

MGI phenotypes (gene matching)
  • abnormal cell death / MGI
  • abnormal somatic motor system morphology / MGI
  • impaired coordination / MGI
  • abnormal dopamine level / MGI
  • decreased susceptibility to pharmacologically induced seizures / MGI
  • no phenotypic analysis / MGI
  • decreased neuron apoptosis / MGI
  • cardiovascular system phenotype / MGI
  • decreased susceptibility to neuronal excitotoxicity / MGI
  • decreased susceptibility to dopaminergic neuron neurotoxicity / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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