IMPC phenotypes (gene matching)
- preweaning lethality, complete penetrance / IMPC
B6.129-Criptotm1Eda/Cnrm
| Status | Available to order |
| EMMA ID | EM:12502 |
| Citation information | RRID:IMSR_EM:12502 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129-Criptotm1Eda/Cnrm |
| Alternative name | Cripto |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Criptotm1Eda |
| Gene/Transgene symbol | Cripto |
Information from provider
| Provider | Giovanna Liguori |
| Provider affiliation | Biomedicine, IGB, CNR |
| Genetic information | Mice carrying a Cripto null allele. The gene was targeted by homologous recombination (Xu et al., 1999) |
| Phenotypic information | Homozygous:No homozygous Cripto -/- mice were born, indicating that Cripto is necessary for embryonic development. Between 9.5 and 10.5 d.p.c. Cripto null embryos consisted primarily of extraembryonic tissue including yolk sacs and the developing placenta, while the embryonic region had degenerated. Embryos initiated gastrulation and some produced mesoderm up to 7.5 d.p.c. Increasingly aberrant morphogenesis gave rise to disordered neuroepithelium that failed to produce a recognizable neural tube, or head-fold (Xu et al., 1999). However, despite the severe phenotype, all the telencephalic, diencephalic, mesencephalic, and isthmic territories investigated are specified in the Cripto -/- embryos, with the exception of the anterior neural ridge. Moreover, neural markers that in wild-type embryos define the more rostral regions, are positioned more distally in Cripto -/- mutants, while markers expressed in the more posterior regions of wild-type embryos are located more proximally in the mutants. A complex neural regionalization of anterior character is detectable inside the Cripto embryos, despite the absence of the node and most of the mesoderm (Liguori et al., 2003). Characterization of the functional properties of the neuroectoderm in mouse Cripto -/- embryos shows that they can still form functional neural stem cells but fail to form functional secondary organizers, probably due to reduced expression of the corresponding signalling molecules (Liguori et al., 2009).Heterozygous:Cripto heterozygotes are healthy and fertile (Xu et al., 1999; Liguori et al., 2003). After chronic treatment with the colonotropic carcinogen azoxymethane (AOM), they develop more numerous and larger colon tumors than wild-type mice, some of them being adenocarcinomas (Giorgio et al., 2014). |
| Breeding history | The mice have been backcrossed to C57BL/6 for more than 20 times. |
| References |
|
| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Alobar holoprosencephaly / Orphanet_93925
- Midline interhemispheric variant of holoprosencephaly / Orphanet_93926
- Microform holoprosencephaly / Orphanet_280200
- Septopreoptic holoprosencephaly / Orphanet_280195
- Semilobar holoprosencephaly / Orphanet_220386
- Lobar holoprosencephaly / Orphanet_93924
MGI phenotypes (gene matching)
- abnormal heart morphology / MGI
- abnormal heart development / MGI
- absent notochord / MGI
- abnormal germ layer development / MGI
- abnormal mesoderm development / MGI
- absent mesoderm / MGI
- abnormal endoderm development / MGI
- failure of primitive streak formation / MGI
- abnormal gastrulation / MGI
- abnormal embryo turning / MGI
- absent vitelline blood vessels / MGI
- embryonic growth arrest / MGI
- premature death / MGI
- abnormal developmental patterning / MGI
- abnormal embryonic tissue morphology / MGI
- abnormal extraembryonic tissue morphology / MGI
- no abnormal phenotype detected / MGI
- abnormal primitive streak formation / MGI
- abnormal chorion morphology / MGI
- abnormal cell adhesion / MGI
- abnormal embryonic neuroepithelium morphology / MGI
- absent prechordal plate / MGI
- abnormal amnion morphology / MGI
- holoprosencephaly / MGI
- cyclopia / MGI
- abnormal rostral-caudal axis patterning / MGI
- embryo phenotype / MGI
- absent somites / MGI
- abnormal mesendoderm development / MGI
- embryonic lethality, complete penetrance / MGI
- embryonic lethality during organogenesis, complete penetrance / MGI
- abnormal visceral endoderm morphology / MGI
- abnormal visceral yolk sac blood island morphology / MGI
- abnormal neural fold morphology / MGI
- decreased fibroblast proliferation / MGI
- fused somites / MGI
- decreased midbrain size / MGI
- decreased forebrain size / MGI
- rostral body truncation / MGI
- short rostral-caudal axis / MGI
- absent anterior definitive endoderm / MGI
Literature references
- Characterization of the functional properties of the neuroectoderm in mouse Cripto(-/-) embryos showing severe gastrulation defects.;Liguori Giovanna L, Echevarria Diego, Bonilla Sonia, D'Andrea Daniela, Liguoro Annamaria, Persico Maria G, Martinez Salvador, ;2009;The International journal of developmental biology;53;549-57; 19247965
- Abrogation of the Cripto gene in mouse leads to failure of postgastrulation morphogenesis and lack of differentiation of cardiomyocytes.;Xu C, Liguori G, Persico M G, Adamson E D, ;1999;Development (Cambridge, England);126;483-94; 9876177
- Anterior neural plate regionalization in cripto null mutant mouse embryos in the absence of node and primitive streak.;Liguori Giovanna L, Echevarría Diego, Improta Raffaele, Signore Massimo, Adamson Eileen, Martínez Salvador, Persico M Graziella, ;2003;Developmental biology;264;537-49; 14651936