IMPC phenotypes (gene matching)
B6;129P2-Hprt1tm1(CAG-EGFP)Laumm/H
| Status | Available to order |
| EMMA ID | EM:12427 |
| International strain name | B6;129P2-Hprt1tm1(CAG-EGFP)Laumm/H |
| Alternative name | LifeAct-GFP |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Hprt1tm1(CAG-EGFP)Laumm |
| Gene/Transgene symbol | Hprt1 |
Information from provider
| Provider | Laura Machesky |
| Provider affiliation | The Beatson Institute for Cancer Research |
| Additional owner | LifecAct plasmid from Ibidi GFP patent owned by GE Healthcare, due to expire in Sept 2021 |
| Genetic information | The cre recombinase-inducible transgenic mice can conditionally express cell-type specific LifeAct-GFP. This is possible due to a floxed neomycin resistance 'STOP' cassette encoded upstream of the LifeAct-GFP fusion cDNA in the Hprt1 locus of the mouse X chromosome. |
| Phenotypic information | Homozygous:No overt phenotype. LifeAct is a 17 amino-acid peptide, isolated from yeast, which facilitates visualisation of filamentous actin (F-actin) in cells and tissues without significantly interfering with actin dynamics. This enables live imaging of the actin cytoskeleton.Heterozygous:No overt phenotype. LifeAct is a 17 amino-acid peptide, isolated from yeast, which facilitates visualisation of filamentous actin (F-actin) in cells and tissues without significantly interfering with actin dynamics. This enables live imaging of the actin cytoskeleton. |
| Breeding history | Intercrossing of LifeAct-GFP mice yielded the expected Mendelian ratios, indicating that no embryonic lethality had occurred. Moreover, LifeAct-GFP male and female mice are fertile and have no major defects in any organs studied. Animals are reproductively mature at 8 weeks of age and a decline in reproductivity can be observed from 6 months of age. Average length of gestation is 19 days. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Disease and phenotype information
MGI phenotypes (gene matching)
- decreased hematocrit / MGI
- increased leukocyte cell number / MGI
- increased neutrophil cell number / MGI
- abnormal small intestine morphology / MGI
- abnormal liver morphology / MGI
- abnormal branching of the mammary ductal tree / MGI
- enlarged spleen / MGI
- spleen hyperplasia / MGI
- enlarged lymph nodes / MGI
- tremors / MGI
- convulsive seizures / MGI
- abnormal lung morphology / MGI
- decreased body weight / MGI
- decreased anxiety-related response / MGI
- ataxia / MGI
- hypoactivity / MGI
- impaired coordination / MGI
- abnormal gait / MGI
- short stride length / MGI
- decreased exploration in new environment / MGI
- limb grasping / MGI
- abnormal motor coordination/balance / MGI
- abnormal hematopoietic system physiology / MGI
- hyperglycemia / MGI
- anemia / MGI
- cardiac hypertrophy / MGI
- increased mammary adenocarcinoma incidence / MGI
- abnormal reflex / MGI
- seizures / MGI
- abnormal motor capabilities/coordination/movement / MGI
- premature death / MGI
- abnormal definitive hematopoiesis / MGI
- abnormal brain morphology / MGI
- no abnormal phenotype detected / MGI
- neurodegeneration / MGI
- abnormal spleen white pulp morphology / MGI
- abnormal hematopoietic system morphology/development / MGI
- abnormal megakaryocyte progenitor cell morphology / MGI
- hepatic steatosis / MGI
- decreased vertical activity / MGI
- increased heart weight / MGI
- increased systemic arterial blood pressure / MGI
- albuminuria / MGI
- decreased erythrocyte cell number / MGI
- increased urine protein level / MGI
- impaired social transmission of food preference / MGI
- no phenotypic analysis / MGI
- phenotypic reversion / MGI
- abnormal dopaminergic neuron morphology / MGI
- astrocytosis / MGI
- abnormal depression-related behavior / MGI
- decreased tumor growth/size / MGI
- abnormal nervous system morphology / MGI
- abnormal cardiac muscle relaxation / MGI
- neuronal intranuclear inclusions / MGI
- abnormal myocardial fiber physiology / MGI
- abnormal Paneth cell morphology / MGI
- decreased B cell number / MGI
- decreased cardiac muscle contractility / MGI
- glomerulosclerosis / MGI
- abnormal podocyte morphology / MGI
- muscle phenotype / MGI
- homeostasis/metabolism phenotype / MGI
- endocrine/exocrine gland phenotype / MGI
- behavior/neurological phenotype / MGI
- immune system phenotype / MGI
- taste/olfaction phenotype / MGI
- hematopoietic system phenotype / MGI
- jerky movement / MGI
- thrombocytosis / MGI
- decreased ventricle muscle contractility / MGI
- decreased mean corpuscular hemoglobin concentration / MGI
- decreased dopamine level / MGI
- abnormal podocyte slit diaphragm morphology / MGI
- absent podocyte slit diaphragm / MGI
- podocyte foot process effacement / MGI
- increased megakaryocyte cell number / MGI
- abnormal spatial reference memory / MGI
- abnormal spatial working memory / MGI
- abnormal splenic cell ratio / MGI
- abnormal physiological response to xenobiotic / MGI
- abnormal enterocyte proliferation / MGI
- abnormal enterocyte apoptosis / MGI
- abnormal neuron differentiation / MGI
- increased mammary gland tumor incidence / MGI
- myeloid hyperplasia / MGI
- expanded mesangial matrix / MGI
- mesangial cell hyperplasia / MGI
- abnormal habituation to a new environment / MGI
- abnormal ceramide level / MGI
- decreased brain choline acetyltransferase activity / MGI
- decreased brain tyrosine 3-monooxygenase activity / MGI
- decreased vascular endothelial cell proliferation / MGI
Literature references
- Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo.;Schachtner Hannah, Li Ang, Stevenson David, Calaminus Simon D J, Thomas Steve, Watson Steve P, Sixt Michael, Wedlich-Soldner Roland, Strathdee Douglas, Machesky Laura M, ;2012;European journal of cell biology;91;923-929; 22658956