C57BL/6NCrl-Abcg8tm1b(KOMP)Wtsi/Ph

Status

Available to order

EMMA IDEM:12339
International strain nameC57BL/6NCrl-Abcg8tm1b(KOMP)Wtsi/Ph
Alternative nameEPD0270_5_F04
Strain typeTargeted Mutant Strains
Allele/Transgene symbolAbcg8tm1b(KOMP)Wtsi
Gene/Transgene symbolAbcg8
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Institute of Molecular Genetics
Provider affiliationDepartment of Transgenic Models of Diseases, Institute of Molecular Genetics
Genetic informationThis mouse line originates from KOMP ES clone EPD0270_5_F04. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele (Gt(ROSA)26Sortm1(ACTB-cre,-EGFP)Ics (MGI:5285392)). Click here for more information on KOMP final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreInstitute of Molecular Genetics, Prague, Czech Republic
Animals used for archivingheterozygous C57BL/6NCrl

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (allele matching)
  • decreased hematocrit / IMPC
  • abnormal liver morphology / IMPC
  • enlarged liver / IMPC
  • abnormal spleen morphology / IMPC
  • enlarged spleen / IMPC
  • abnormal lens morphology / IMPC
  • impaired righting response / IMPC
  • increased circulating triglyceride level / IMPC
  • male infertility / IMPC
  • female infertility / IMPC
  • decreased mean corpuscular volume / IMPC
  • increased mean platelet volume / IMPC
  • decreased hemoglobin content / IMPC
  • increased circulating alanine transaminase level / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • abnormal heart left ventricle morphology / IMPC
  • decreased circulating iron level / IMPC
  • decreased T cell number / IMPC
  • increased circulating cholesterol level / IMPC
  • abnormal cholesterol homeostasis / IMPC
  • increased circulating aspartate transaminase level / IMPC
  • immune system phenotype / IMPC
  • decreased circulating serum albumin level / IMPC
  • increased circulating creatinine level / IMPC
  • decreased mean corpuscular hemoglobin / IMPC
  • increased cardiac muscle contractility / IMPC
  • increased circulating potassium level / IMPC
  • decreased circulating sodium level / IMPC
  • decreased mean corpuscular hemoglobin concentration / IMPC
  • decreased NK T cell number / IMPC
  • decreased CD4-positive, alpha-beta T cell number / IMPC
  • decreased CD8-positive, alpha-beta T cell number / IMPC
  • decreased B-2 B cell number / IMPC
  • increased red blood cell distribution width / IMPC
  • abnormal retina blood vessel morphology / IMPC
  • increased lung elastance / IMPC
  • decreased CD4-negative NK T cell number / IMPC
  • decreased memory-marker CD4-negative NK T cell number / IMPC
IMPC phenotypes (gene matching)
  • decreased circulating serum albumin level / IMPC
  • increased red blood cell distribution width / IMPC
  • increased circulating creatinine level / IMPC
  • abnormal liver morphology / IMPC
  • increased cardiac muscle contractility / IMPC
  • increased circulating aspartate transaminase level / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • increased circulating cholesterol level / IMPC
  • immune system phenotype / IMPC
  • decreased NK T cell number / IMPC
  • enlarged spleen / IMPC
  • increased circulating alanine transaminase level / IMPC
  • increased lung elastance / IMPC
  • enlarged liver / IMPC
  • increased mean platelet volume / IMPC
  • decreased mean corpuscular volume / IMPC
  • abnormal spleen morphology / IMPC
  • abnormal heart left ventricle morphology / IMPC
  • decreased CD8-positive, alpha-beta T cell number / IMPC
  • abnormal retina blood vessel morphology / IMPC
  • decreased CD4-positive, alpha-beta T cell number / IMPC
  • decreased mean corpuscular hemoglobin / IMPC
  • abnormal cholesterol homeostasis / IMPC
  • female infertility / IMPC
  • abnormal lens morphology / IMPC
  • impaired righting response / IMPC
  • decreased circulating iron level / IMPC
  • increased circulating triglyceride level / IMPC
  • decreased mean corpuscular hemoglobin concentration / IMPC
  • decreased memory-marker CD4-negative NK T cell number / IMPC
  • male infertility / IMPC
  • decreased circulating sodium level / IMPC
  • decreased CD4-negative NK T cell number / IMPC
  • decreased hematocrit / IMPC
  • decreased T cell number / IMPC
  • decreased B-2 B cell number / IMPC
  • increased circulating potassium level / IMPC
  • decreased hemoglobin content / IMPC
MGI phenotypes (gene matching)
  • increased circulating triglyceride level / MGI
  • abnormal hepatobiliary system physiology / MGI
  • decreased cholesterol level / MGI
  • decreased circulating cholesterol level / MGI
  • increased circulating phytosterol level / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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