B6.FVB-Tg(Gjb6-cre/ERT2)53-33Fwp/Orl
| Status | Available to order |
| EMMA ID | EM:12217 |
| International strain name | B6.FVB-Tg(Gjb6-cre/ERT2)53-33Fwp/Orl |
| Alternative name | Tg(Gjb6-cre/ERT2)53-33Fwp |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Gjb6-cre/ERT2)53-33Fwp |
| Gene/Transgene symbol | Tg(Gjb6-cre/ERT2)53-33Fwp |
Information from provider
| Provider | Frank Pfrieger |
| Provider affiliation | Institute of Cellular and Integrative Neurosciences CNRS UPR 3212 |
| Additional owner | Prof. Pierre Chambon, IGBMC, Illkirch, France |
| Genetic information | The creERT2 expression cassette, preceded by a rabbit beta-globin DNA encompassing intron 2 (beta-globin intervening sequences [bGIVS]) and followed by the SV40 polyA signal and a PGK/Tn5-driven neomycin resistance cassette flanked by FRT sites (neo) was inserted by homologous recombination at the transcription initiation site of the Gjb6 gene located in BAC RP24-368M7. The linearized vector was injected into pronuclei of fertilized FVB/N eggs. |
| Phenotypic information | Homozygous:The line allows for inducible somatic mutagenesis in Gjb6-/Cx30-expressing cells, notably astrocytes in different regions of the central nervous system.Heterozygous:The line allows for inducible somatic mutagenesis in Gjb6-/Cx30-expressing cells, notably astrocytes in different regions of the central nervous system. |
| Breeding history | Backcrossed to C57BL/6 for more than 10 generations |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
Literature references
- Transgenic mice for conditional gene manipulation in astroglial cells.;Slezak Michal, Göritz Christian, Niemiec Aurore, Frisén Jonas, Chambon Pierre, Metzger Daniel, Pfrieger Frank W, ;2007;Glia;55;1565-76; 17823970
- Origin of new glial cells in intact and injured adult spinal cord.;Barnabé-Heider Fanie, Göritz Christian, Sabelström Hanna, Takebayashi Hirohide, Pfrieger Frank W, Meletis Konstantinos, Frisén Jonas, ;2010;Cell stem cell;7;470-82; 20887953
- Role of astroglial connexin30 in hippocampal gap junction coupling.;Gosejacob Dominic, Dublin Pavel, Bedner Peter, Hüttmann Kerstin, Zhang Jiong, Tress Oliver, Willecke Klaus, Pfrieger Frank, Steinhäuser Christian, Theis Martin, ;2011;Glia;59;511-9; 21264956
- Calcium dynamics in astrocyte processes during neurovascular coupling.;Otsu Yo, Couchman Kiri, Lyons Declan G, Collot Mayeul, Agarwal Amit, Mallet Jean-Maurice, Pfrieger Frank W, Bergles Dwight E, Charpak Serge, ;2015;Nature neuroscience;18;210-8; 25531572
- An autocrine purinergic signaling controls astrocyte-induced neuronal excitation.;Shen Weida, Nikolic Ljiljana, Meunier Claire, Pfrieger Frank, Audinat Etienne, ;2017;Scientific reports;7;11280; 28900295
- Glucocorticoid receptor in astrocytes regulates midbrain dopamine neurodegeneration through connexin hemichannel activity.;Maatouk Layal, Yi Chenju, Carrillo-de Sauvage Maria-Angeles, Compagnion Anne-Claire, Hunot Stéphane, Ezan Pascal, Hirsch Etienne C, Koulakoff Annette, Pfrieger Frank W, Tronche François, Leybaert Luc, Giaume Christian, Vyas Sheela, ;2019;Cell death and differentiation;26;580-596; 30006609
- Astrocyte-derived Jagged-1 mitigates deleterious Notch signaling in amyotrophic lateral sclerosis.;Nonneman Annelies, Criem Nathan, Lewandowski Sebastian A, Nuyts Rik, Thal Dietmar R, Pfrieger Frank W, Ravits John, Van Damme Philip, Zwijsen An, Van Den Bosch Ludo, Robberecht Wim, ;2018;Neurobiology of disease;119;26-40; 30010003