B6.FVB-Tg(Gjb6-cre/ERT2)53-33Fwp/Orl
| Status | Available to order |
| EMMA ID | EM:12217 |
| Citation information | RRID:IMSR_EM:12217 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.FVB-Tg(Gjb6-cre/ERT2)53-33Fwp/Orl |
| Alternative name | Tg(Gjb6-cre/ERT2)53-33Fwp |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Gjb6-cre/ERT2)53-33Fwp |
| Gene/Transgene symbol | Tg(Gjb6-cre/ERT2)53-33Fwp |
Information from provider
| Provider | Frank Pfrieger |
| Provider affiliation | Institute of Cellular and Integrative Neurosciences CNRS UPR 3212 |
| Additional owner | Prof. Pierre Chambon, IGBMC, Illkirch, France |
| Genetic information | The creERT2 expression cassette, preceded by a rabbit beta-globin DNA encompassing intron 2 (beta-globin intervening sequences [bGIVS]) and followed by the SV40 polyA signal and a PGK/Tn5-driven neomycin resistance cassette flanked by FRT sites (neo) was inserted by homologous recombination at the transcription initiation site of the Gjb6 gene located in BAC RP24-368M7. The linearized vector was injected into pronuclei of fertilized FVB/N eggs. |
| Phenotypic information | Homozygous:The line allows for inducible somatic mutagenesis in Gjb6-/Cx30-expressing cells, notably astrocytes in different regions of the central nervous system.Heterozygous:The line allows for inducible somatic mutagenesis in Gjb6-/Cx30-expressing cells, notably astrocytes in different regions of the central nervous system. |
| Breeding history | Backcrossed to C57BL/6 for more than 10 generations |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
Literature references
- Transgenic mice for conditional gene manipulation in astroglial cells.;Slezak Michal, Göritz Christian, Niemiec Aurore, Frisén Jonas, Chambon Pierre, Metzger Daniel, Pfrieger Frank W, ;2007;Glia;55;1565-76; 17823970
- Origin of new glial cells in intact and injured adult spinal cord.;Barnabé-Heider Fanie, Göritz Christian, Sabelström Hanna, Takebayashi Hirohide, Pfrieger Frank W, Meletis Konstantinos, Frisén Jonas, ;2010;Cell stem cell;7;470-82; 20887953
- Role of astroglial connexin30 in hippocampal gap junction coupling.;Gosejacob Dominic, Dublin Pavel, Bedner Peter, Hüttmann Kerstin, Zhang Jiong, Tress Oliver, Willecke Klaus, Pfrieger Frank, Steinhäuser Christian, Theis Martin, ;2011;Glia;59;511-9; 21264956
- Calcium dynamics in astrocyte processes during neurovascular coupling.;Otsu Yo, Couchman Kiri, Lyons Declan G, Collot Mayeul, Agarwal Amit, Mallet Jean-Maurice, Pfrieger Frank W, Bergles Dwight E, Charpak Serge, ;2015;Nature neuroscience;18;210-8; 25531572
- An autocrine purinergic signaling controls astrocyte-induced neuronal excitation.;Shen Weida, Nikolic Ljiljana, Meunier Claire, Pfrieger Frank, Audinat Etienne, ;2017;Scientific reports;7;11280; 28900295
- Glucocorticoid receptor in astrocytes regulates midbrain dopamine neurodegeneration through connexin hemichannel activity.;Maatouk Layal, Yi Chenju, Carrillo-de Sauvage Maria-Angeles, Compagnion Anne-Claire, Hunot Stéphane, Ezan Pascal, Hirsch Etienne C, Koulakoff Annette, Pfrieger Frank W, Tronche François, Leybaert Luc, Giaume Christian, Vyas Sheela, ;2019;Cell death and differentiation;26;580-596; 30006609
- Astrocyte-derived Jagged-1 mitigates deleterious Notch signaling in amyotrophic lateral sclerosis.;Nonneman Annelies, Criem Nathan, Lewandowski Sebastian A, Nuyts Rik, Thal Dietmar R, Pfrieger Frank W, Ravits John, Van Damme Philip, Zwijsen An, Van Den Bosch Ludo, Robberecht Wim, ;2018;Neurobiology of disease;119;26-40; 30010003