B6.FVB-Tg(Slc1a3-cre/ERT2)45-72Fwp/Orl

Status	Available to order
EMMA ID	EM:12216
International strain name	B6.FVB-Tg(Slc1a3-cre/ERT2)45-72Fwp/Orl
Alternative name	Tg(Slc1a3-cre/ERT2)45-72Fwp
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(Slc1a3-cre/ERT2)45-72Fwp
Gene/Transgene symbol	Tg(Slc1a3-cre/ERT2)45-72Fwp

Information from provider

Provider	Frank Pfrieger
Provider affiliation	Institute of Cellular and Integrative Neurosciences CNRS UPR 3212
Additional owner	Prof. Pierre Chambon, IGBMC, Illkirch, France
Genetic information	The creERT2 expression cassette, preceded by a rabbit beta-globin DNA encompassing intron 2 (beta-globin intervening sequences [bGIVS]) and followed by the SV40 polyA signal and a PGK/Tn5-driven neomycin resistance cassette flanked by FRT sites (neo) was inserted by homologous recombination at the transcription initiation site of the Slc1a3 (Glast) gene located in BAC RP23-63O21. The linearized vector was injected into pronuclei of fertilized FVB/N eggs.
Phenotypic information	Homozygous: Transgenic line for inducible somatic mutagenesis in Slc1a3/Glast-expressing cells, notably astroglial cells throughout the brain, adult neural cells/progenitors in neurogenic regions and retinal astroglial cells of the retina. Heterozygous: Transgenic line for inducible somatic mutagenesis in Slc1a3/Glast-expressing cells, notably astroglial cells throughout the brain, adult neural cells/progenitors in neurogenic regions and retinal astroglial cells of the retina.
Breeding history	Backcrossed on C57BL/6 for more than 10 generations
References	Transgenic mice for conditional gene manipulation in astroglial cells.;Slezak Michal, Göritz Christian, Niemiec Aurore, Frisén Jonas, Chambon Pierre, Metzger Daniel, Pfrieger Frank W, ;2007;Glia;55;1565-76; 17823970 Origin of new glial cells in intact and injured adult spinal cord.;Barnabé-Heider Fanie, Göritz Christian, Sabelström Hanna, Takebayashi Hirohide, Pfrieger Frank W, Meletis Konstantinos, Frisén Jonas, ;2010;Cell stem cell;7;470-82; 20887953 RBPJkappa-dependent signaling is essential for long-term maintenance of neural stem cells in the adult hippocampus.;Ehm Oliver, Göritz Christian, Covic Marcela, Schäffner Iris, Schwarz Tobias J, Karaca Esra, Kempkes Bettina, Kremmer Elisabeth, Pfrieger Frank W, Espinosa Lluis, Bigas Anna, Giachino Claudio, Taylor Verdon, Frisén Jonas, Lie D Chichung, ;2010;The Journal of neuroscience : the official journal of the Society for Neuroscience;30;13794-807; 20943920 A pericyte origin of spinal cord scar tissue.;Göritz Christian, Dias David O, Tomilin Nikolay, Barbacid Mariano, Shupliakov Oleg, Frisén Jonas, ;2011;Science (New York, N.Y.);333;238-42; 21737741 The Role of Hypothalamic NF-κB Signaling in the Response of the HPT-Axis to Acute Inflammation in Female Mice.;de Vries E M, Nagel S, Haenold R, Sundaram S M, Pfrieger F W, Fliers E, Heuer H, Boelen A, ;2016;Endocrinology;157;2947-56; 27187176
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Institut de Transgenose, INTRAGENE, Orléans, France
Animals used for archiving	heterozygous C57BL/6J

Literature references

Transgenic mice for conditional gene manipulation in astroglial cells.;Slezak Michal, Göritz Christian, Niemiec Aurore, Frisén Jonas, Chambon Pierre, Metzger Daniel, Pfrieger Frank W, ;2007;Glia;55;1565-76; 17823970
Origin of new glial cells in intact and injured adult spinal cord.;Barnabé-Heider Fanie, Göritz Christian, Sabelström Hanna, Takebayashi Hirohide, Pfrieger Frank W, Meletis Konstantinos, Frisén Jonas, ;2010;Cell stem cell;7;470-82; 20887953
RBPJkappa-dependent signaling is essential for long-term maintenance of neural stem cells in the adult hippocampus.;Ehm Oliver, Göritz Christian, Covic Marcela, Schäffner Iris, Schwarz Tobias J, Karaca Esra, Kempkes Bettina, Kremmer Elisabeth, Pfrieger Frank W, Espinosa Lluis, Bigas Anna, Giachino Claudio, Taylor Verdon, Frisén Jonas, Lie D Chichung, ;2010;The Journal of neuroscience : the official journal of the Society for Neuroscience;30;13794-807; 20943920
A pericyte origin of spinal cord scar tissue.;Göritz Christian, Dias David O, Tomilin Nikolay, Barbacid Mariano, Shupliakov Oleg, Frisén Jonas, ;2011;Science (New York, N.Y.);333;238-42; 21737741
The Role of Hypothalamic NF-κB Signaling in the Response of the HPT-Axis to Acute Inflammation in Female Mice.;de Vries E M, Nagel S, Haenold R, Sundaram S M, Pfrieger F W, Fliers E, Heuer H, Boelen A, ;2016;Endocrinology;157;2947-56; 27187176

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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