B6;D2-Tg(St18-cre/ERT2)1Ffr/Orl

Status

Available to order

EMMA IDEM:12206
International strain nameB6;D2-Tg(St18-cre/ERT2)1Ffr/Orl
Alternative nameTg St18-CreERT2 C57BL/6J
Strain typeTransgenic Strains
Allele/Transgene symbolTg(St18-cre/ERT2)1Ffr
Gene/Transgene symbolTg(St18-cre/ERT2)1Ffr

Information from provider

ProviderFiona Francis
Provider affiliationInstitut du Fer à Moulin, Inserm UMR-S 839
Genetic informationTransgenic line produced by classical transgenesis (microinjection into B6D2F2 (B6D2F1 x B6D2F1)) using a modified BAC clone (RPCI-23-2303) containing the "suppression of tumorigenicity 18" (St18) gene. BAC recombining was used to insert the CreERT2 FRT Neo FRT cassette by homologous recombination into the genomic St18 locus.
Phenotypic informationHomozygous:
Not yet tested but likely to be normal.

Heterozygous:
Normal. No obvious phenotype detected. Mice are viable and fertile. These mice have been crossed with a reporter line and the CreERT2 expression (induced by tamoxifen) faithfully mimics the St18 in situ hybridisation result. We wish to deposit this heterozygote line in EMMA.
Breeding historyA Tg St18-CreERT2 male founder was crossed with wild-type C57BL/6J females to generate 5 heterozygote offspring (of 8 born in July 2018) (two males, three females). Females are being re-crossed with wild type C57BL/6J mice.
ReferencesNone available
Homozygous fertilenot known
Homozygous viablenot known
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France
Animals used for archivingheterozygous C57BL/6J

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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