B6Brd;B6N-Tyr^c-Brd Cldn16^{tm1a(KOMP)Wtsi}/Wtsi

Status	Available to order
EMMA ID	EM:12157
International strain name	B6Brd;B6N-Tyr^c-Brd Cldn16^{tm1a(KOMP)Wtsi}/Wtsi
Alternative name	EPD0150_2_B12
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Cldn16^{tm1a(KOMP)Wtsi}
Gene/Transgene symbol	Cldn16
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from KOMP ES clone EPD0150_2_B12. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131 A gene expression resource generated by genome-wide lacZ profiling in the mouse.;Tuck Elizabeth, Estabel Jeanne, Oellrich Anika, Maguire Anna Karin, Adissu Hibret A, Souter Luke, Siragher Emma, Lillistone Charlotte, Green Angela L, Wardle-Jones Hannah, Carragher Damian M, Karp Natasha A, Smedley Damian, Adams Niels C, null null, Bussell James N, Adams David J, Ramírez-Solis Ramiro, Steel Karen P, Galli Antonella, White Jacqueline K, ;2015;Disease models & mechanisms;8;1467-78; 26398943

Information from EMMA

Archiving centre	Wellcome Trust Sanger Institute, Hinxton, United Kingdom
Animals used for archiving	homozygous C57BL/6Brd-Tyr;C57BL/6N

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Familial primary hypomagnesemia with hypercalciuria and nephrocalcinosis without severe ocular involvement / Orphanet_31043

IMPC phenotypes (allele matching)

increased circulating calcium level / IMPC
increased circulating creatinine level / IMPC
increased circulating amylase level / IMPC
process of degenerative change / IMPC
lipid deposition / IMPC
hydronephrosis / IMPC

IMPC phenotypes (gene matching)

process of degenerative change / IMPC
lipid deposition / IMPC
increased circulating amylase level / IMPC
hydronephrosis / IMPC
increased circulating calcium level / IMPC
increased circulating creatinine level / IMPC

MGI phenotypes (gene matching)

increased circulating parathyroid hormone level / MGI
increased urine calcium level / MGI
decreased urine pH / MGI
decreased circulating magnesium level / MGI
abnormal renal reabsorbtion / MGI
abnormal renal calcium reabsorbtion / MGI
abnormal vitamin D level / MGI

Literature references

Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131
A gene expression resource generated by genome-wide lacZ profiling in the mouse.;Tuck Elizabeth, Estabel Jeanne, Oellrich Anika, Maguire Anna Karin, Adissu Hibret A, Souter Luke, Siragher Emma, Lillistone Charlotte, Green Angela L, Wardle-Jones Hannah, Carragher Damian M, Karp Natasha A, Smedley Damian, Adams Niels C, null null, Bussell James N, Adams David J, Ramírez-Solis Ramiro, Steel Karen P, Galli Antonella, White Jacqueline K, ;2015;Disease models & mechanisms;8;1467-78; 26398943

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6Brd;B6N-Tyrc-Brd Cldn16tm1a(KOMP)Wtsi/Wtsi