B6Brd;B6N-Tyr^c-Brd Nfkb1^{tm1a(KOMP)Wtsi}/Wtsi

Status	Available to order
EMMA ID	EM:12090
International strain name	B6Brd;B6N-Tyr^c-Brd Nfkb1^{tm1a(KOMP)Wtsi}/Wtsi
Alternative name	EPD0033_4_D01
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Nfkb1^{tm1a(KOMP)Wtsi}
Gene/Transgene symbol	Nfkb1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from KOMP ES clone EPD0033_4_D01. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centre	Wellcome Trust Sanger Institute, Hinxton, United Kingdom
Animals used for archiving	homozygous C57BL/6Dnk;C57BL/6Brd-Tyr;C57BL/6N

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Common variable immunodeficiency / Orphanet_1572

IMPC phenotypes (allele matching)

increased circulating LDL cholesterol level / IMPC
increased leukocyte cell number / IMPC
abnormal skin condition / IMPC
decreased body length / IMPC
abnormal cornea morphology / IMPC
increased circulating free fatty acids level / IMPC
abnormal coat/hair pigmentation / IMPC
increased circulating alanine transaminase level / IMPC
increased lactate dehydrogenase level / IMPC
increased circulating alkaline phosphatase level / IMPC
decreased lean body mass / IMPC
increased circulating aspartate transaminase level / IMPC
decreased circulating serum albumin level / IMPC
decreased mean corpuscular hemoglobin / IMPC
decreased circulating sodium level / IMPC
increased circulating iron level / IMPC

IMPC phenotypes (gene matching)

decreased lean body mass / IMPC
increased leukocyte cell number / IMPC
increased circulating alkaline phosphatase level / IMPC
increased circulating LDL cholesterol level / IMPC
abnormal skin condition / IMPC
decreased mean corpuscular hemoglobin / IMPC
decreased circulating sodium level / IMPC
decreased circulating serum albumin level / IMPC
abnormal coat/hair pigmentation / IMPC
increased circulating free fatty acids level / IMPC
increased circulating aspartate transaminase level / IMPC
decreased body length / IMPC
increased circulating alanine transaminase level / IMPC
abnormal cornea morphology / IMPC
increased lactate dehydrogenase level / IMPC
increased circulating iron level / IMPC

MGI phenotypes (allele matching)

increased leukocyte cell number / MGI
lymphoid hyperplasia / MGI
decreased IgA level / MGI
increased susceptibility to bacterial infection / MGI
increased circulating alkaline phosphatase level / MGI
decreased regulatory T cell number / MGI
decreased NK cell number / MGI
decreased IgG1 level / MGI
decreased respiratory quotient / MGI
increased circulating free fatty acid level / MGI
decreased lean body mass / MGI
decreased mean corpuscular hemoglobin concentration / MGI
decreased circulating amylase level / MGI

MGI phenotypes (gene matching)

increased leukocyte cell number / MGI
extramedullary hematopoiesis / MGI
abnormal colon morphology / MGI
lymphoid hyperplasia / MGI
enlarged spleen / MGI
enlarged lymph nodes / MGI
increased circulating free fatty acid level / MGI
postnatal growth retardation / MGI
abnormal humoral immune response / MGI
decreased IgG level / MGI
decreased IgM level / MGI
decreased IgA level / MGI
abnormal inflammatory response / MGI
liver inflammation / MGI
lung inflammation / MGI
premature death / MGI
no abnormal phenotype detected / MGI
abnormal lymph organ size / MGI
abnormal inguinal lymph node morphology / MGI
abnormal dendritic cell physiology / MGI
abnormal Peyer's patch follicle morphology / MGI
abnormal Peyer's patch germinal center morphology / MGI
abnormal Peyer's patch T cell area morphology / MGI
abnormal bone marrow cell morphology/development / MGI
increased susceptibility to bacterial infection / MGI
abnormal macrophage physiology / MGI
abnormal professional antigen presenting cell physiology / MGI
abnormal B cell physiology / MGI
decreased immunoglobulin level / MGI
increased immunoglobulin level / MGI
decreased IgE level / MGI
increased IgE level / MGI
cochlear ganglion degeneration / MGI
decreased erythrocyte cell number / MGI
increased circulating alkaline phosphatase level / MGI
abnormal cytokine secretion / MGI
peritoneal inflammation / MGI
large intestinal inflammation / MGI
abnormal lymphocyte physiology / MGI
decreased lean body mass / MGI
abnormal calcium ion homeostasis / MGI
abnormal cochlear inner hair cell morphology / MGI
decreased cochlear nerve compound action potential / MGI
increased susceptibility to noise-induced hearing loss / MGI
increased susceptibility to age-related hearing loss / MGI
decreased susceptibility to autoimmune diabetes / MGI
abnormal class switch recombination / MGI
decreased regulatory T cell number / MGI
decreased B-1 B cell number / MGI
decreased osteoclast cell number / MGI
diarrhea / MGI
decreased B cell proliferation / MGI
decreased T cell proliferation / MGI
increased B cell proliferation / MGI
lethargy / MGI
muscle phenotype / MGI
homeostasis/metabolism phenotype / MGI
immune system phenotype / MGI
skeleton phenotype / MGI
abnormal CD4-positive, alpha-beta T cell physiology / MGI
decreased mean corpuscular hemoglobin concentration / MGI
increased apoptosis / MGI
decreased NK T cell number / MGI
decreased NK cell number / MGI
decreased memory T cell number / MGI
decreased single-positive T cell number / MGI
decreased myeloid dendritic cell number / MGI
decreased Peyer's patch number / MGI
small Peyer's patches / MGI
decreased marginal zone B cell number / MGI
decreased IgG1 level / MGI
decreased IgG3 level / MGI
increased IgG1 level / MGI
increased susceptibility to induced colitis / MGI
decreased tumor necrosis factor secretion / MGI
decreased circulating interleukin-2 level / MGI
decreased interleukin-2 secretion / MGI
decreased interleukin-4 secretion / MGI
decreased interleukin-6 secretion / MGI
decreased circulating amylase level / MGI
cecum inflammation / MGI
absent inguinal lymph nodes / MGI
small inguinal lymph nodes / MGI
increased susceptibility to bacterial infection induced morbidity/mortality / MGI
decreased susceptibility to viral infection induced morbidity/mortality / MGI
rough coat / MGI
myeloid hyperplasia / MGI
decreased respiratory quotient / MGI
decreased central memory CD4-positive, alpha-beta T cell number / MGI
decreased effector memory CD4-positive, alpha-beta T cell number / MGI
increased central memory CD8 positive, alpha-beta T cell number / MGI
preweaning lethality, incomplete penetrance / MGI
decreased CD8-positive, naive alpha-beta T cell number / MGI
impaired humoral immune response / MGI

Literature references

Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6Brd;B6N-Tyrc-Brd Nfkb1tm1a(KOMP)Wtsi/Wtsi