B6Brd;B6N-Tyr^c-Brd Sytl1^{tm1a(KOMP)Wtsi}/Wtsi

Status	Available to order
EMMA ID	EM:12069
International strain name	B6Brd;B6N-Tyr^c-Brd Sytl1^{tm1a(KOMP)Wtsi}/Wtsi
Alternative name	EPD0084_1_C03
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Sytl1^{tm1a(KOMP)Wtsi}
Gene/Transgene symbol	Sytl1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from KOMP ES clone EPD0084_1_C03. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centre	Wellcome Trust Sanger Institute, Hinxton, United Kingdom
Animals used for archiving	homozygous C57BL/6Brd-Tyr;C57BL/6N

Disease and phenotype information

IMPC phenotypes (allele matching)

increased leukocyte cell number / IMPC
abnormal coat/hair pigmentation / IMPC
increased hematocrit / IMPC
abnormal response to tactile stimuli / IMPC
increased blood uric acid level / IMPC

IMPC phenotypes (gene matching)

abnormal coat/hair pigmentation / IMPC
increased leukocyte cell number / IMPC
increased hematocrit / IMPC
increased blood uric acid level / IMPC
abnormal response to tactile stimuli / IMPC

MGI phenotypes (allele matching)

increased mean platelet volume / MGI
vertebral transformation / MGI
abnormal locomotor coordination / MGI
epididymal inflammation / MGI
caudal vertebral transformation / MGI
increased regulatory T cell number / MGI
decreased T cell number / MGI
CNS inflammation / MGI
decreased CD4-positive, alpha beta T cell number / MGI
decreased mature B cell number / MGI
decreased IgG2b level / MGI
decreased circulating amylase level / MGI
increased hematocrit / MGI
decreased hemoglobin content / MGI

MGI phenotypes (gene matching)

increased mean platelet volume / MGI
increased hematocrit / MGI
abnormal pancreas secretion / MGI
decreased hemoglobin content / MGI
vertebral transformation / MGI
decreased insulin secretion / MGI
abnormal locomotor coordination / MGI
epididymal inflammation / MGI
caudal vertebral transformation / MGI
increased regulatory T cell number / MGI
decreased T cell number / MGI
homeostasis/metabolism phenotype / MGI
CNS inflammation / MGI
decreased CD4-positive, alpha beta T cell number / MGI
decreased mature B cell number / MGI
decreased IgG2b level / MGI
decreased circulating amylase level / MGI
pancreatic acinar cell zymogen granule accumulation / MGI

Literature references

Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6Brd;B6N-Tyrc-Brd Sytl1tm1a(KOMP)Wtsi/Wtsi