B6Brd;B6N-Tyr^c-Brd Zc3hc1^{tm1a(KOMP)Wtsi}/Wtsi

Status	Available to order
EMMA ID	EM:12021
International strain name	B6Brd;B6N-Tyr^c-Brd Zc3hc1^{tm1a(KOMP)Wtsi}/Wtsi
Alternative name	EPD0025_3_C05
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Zc3hc1^{tm1a(KOMP)Wtsi}
Gene/Transgene symbol	Zc3hc1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from KOMP ES clone EPD0025_3_C05. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centre	Wellcome Trust Sanger Institute, Hinxton, United Kingdom
Animals used for archiving	heterozygous C57BL/6Dnk;C57BL/6Brd-Tyr;C57BL/6N

Disease and phenotype information

IMPC phenotypes (allele matching)

abnormal cranium morphology / IMPC
abnormal snout morphology / IMPC
decreased body weight / IMPC
abnormal cornea morphology / IMPC
abnormal posture / IMPC
male infertility / IMPC
abnormal tooth morphology / IMPC
decreased lean body mass / IMPC
hypoplasia / IMPC
process of degenerative change / IMPC

IMPC phenotypes (gene matching)

hypoplasia / IMPC
abnormal cranium morphology / IMPC
decreased body weight / IMPC
abnormal snout morphology / IMPC
abnormal cornea morphology / IMPC
abnormal tooth morphology / IMPC
abnormal posture / IMPC
male infertility / IMPC
process of degenerative change / IMPC
decreased lean body mass / IMPC

MGI phenotypes (allele matching)

abnormal snout morphology / MGI
decreased body weight / MGI
abnormal cornea morphology / MGI
decreased rib number / MGI
decreased lean body mass / MGI
decreased thoracic vertebrae number / MGI

MGI phenotypes (gene matching)

abnormal snout morphology / MGI
abnormal ovary morphology / MGI
abnormal testis morphology / MGI
small seminiferous tubules / MGI
arrest of spermatogenesis / MGI
abnormal spermatogenesis / MGI
decreased body weight / MGI
decreased body size / MGI
abnormal cornea morphology / MGI
abnormal apoptosis / MGI
reduced female fertility / MGI
male infertility / MGI
abnormal meiosis / MGI
ovary cysts / MGI
decreased corpora lutea number / MGI
no phenotypic analysis / MGI
testicular atrophy / MGI
decreased rib number / MGI
abnormal female reproductive system physiology / MGI
decreased lean body mass / MGI
decreased thoracic vertebrae number / MGI
decreased testis weight / MGI
decreased male germ cell number / MGI
azoospermia / MGI
abnormal female meiosis / MGI
decreased oocyte number / MGI
abnormal spermatocyte morphology / MGI
abnormal spermatid morphology / MGI
arrest of male meiosis / MGI
abnormal male germ cell apoptosis / MGI
abnormal chromosomal synapsis / MGI
abnormal synaptonemal complex / MGI
lethality, incomplete penetrance / MGI
abnormal double-strand DNA break repair / MGI
decreased primordial ovarian follicle number / MGI

Literature references

Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6Brd;B6N-Tyrc-Brd Zc3hc1tm1a(KOMP)Wtsi/Wtsi