B6Brd;B6N-Tyr^c-Brd Gmnn^{tm1a(KOMP)Wtsi}/Wtsi

Status	Available to order
EMMA ID	EM:12010
International strain name	B6Brd;B6N-Tyr^c-Brd Gmnn^{tm1a(KOMP)Wtsi}/Wtsi
Alternative name	EPD0033_2_D07
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Gmnn^{tm1a(KOMP)Wtsi}
Gene/Transgene symbol	Gmnn
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from KOMP ES clone EPD0033_2_D07. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centre	Wellcome Trust Sanger Institute, Hinxton, United Kingdom
Animals used for archiving	heterozygous C57BL/6Dnk;C57BL/6Brd-Tyr;C57BL/6N, wild-type C57BL/6N
Stage of embryos	2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Ear-patella-short stature syndrome / Orphanet_2554

IMPC phenotypes (allele matching)

abnormal lens morphology / IMPC
cataract / IMPC
preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

abnormal lens morphology / IMPC
preweaning lethality, complete penetrance / IMPC
cataract / IMPC

MGI phenotypes (gene matching)

decreased cell proliferation / MGI
embryonic growth arrest / MGI
no abnormal phenotype detected / MGI
abnormal inner cell mass morphology / MGI
abnormal cell nucleus morphology / MGI
abnormal cell adhesion / MGI
abnormal mitosis / MGI
absent inner cell mass / MGI
abnormal trophectoderm morphology / MGI
abnormal cell differentiation / MGI
abnormal preimplantation embryo development / MGI
embryonic lethality before implantation, complete penetrance / MGI
embryonic lethality between implantation and somite formation, complete penetrance / MGI
absent trophectoderm / MGI

Literature references

Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6Brd;B6N-Tyrc-Brd Gmnntm1a(KOMP)Wtsi/Wtsi