IMPC phenotypes (gene matching)
B6.129P2(CD1)-Boctm1Aok/Cnbc
| Status | Available to order |
| EMMA ID | EM:11898 |
| Citation information | RRID:IMSR_EM:11898 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129P2(CD1)-Boctm1Aok/Cnbc |
| Alternative name | Boc- |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Boctm1Aok |
| Gene/Transgene symbol | Boc |
Information from provider
| Provider | Ami Okada |
| Provider affiliation | Department of Biological Sciences, Stanford University |
| Genetic information | Homologous recombination was used to insert a gene trap vector encoding a strong splice acceptor site, transmembrane domain and beta-geo followed by an IRES-PLAP upstream of the exon encoding the transmembrane domain. The allele proved to be severely hypomorphic. |
| Phenotypic information | Homozygous:Nervous system, axonal circuit alterationsHeterozygous:Nervous system, axonal circuit alterations |
| Breeding history | Mutation first introduced through E14 (ES Cell) 129P2/OlaHsd, then bred to CD1 and eventually backcrossed to C57BL/6J for more than 10 generations. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | homozygous C57BL/6J males, wild-type C57BL/6J females |
| Stage of embryos | 2-cell |
Disease and phenotype information
Literature references
- Boc is a receptor for sonic hedgehog in the guidance of commissural axons.;Okada Ami, Charron Frédéric, Morin Steves, Shin David S, Wong Karen, Fabre Pierre J, Tessier-Lavigne Marc, McConnell Susan K, ;2006;Nature;444;369-73; 17086203
- Secreted frizzled related proteins modulate pathfinding and fasciculation of mouse retina ganglion cell axons by direct and indirect mechanisms.;Marcos Séverine, Nieto-Lopez Francisco, Sandonìs Africa, Cardozo Marcos Julian, Di Marco Fabiana, Esteve Pilar, Bovolenta Paola, ;2015;The Journal of neuroscience : the official journal of the Society for Neuroscience;35;4729-40; 25788689
- Segregation of ipsilateral retinal ganglion cell axons at the optic chiasm requires the Shh receptor Boc.;Fabre Pierre J, Shimogori Tomomi, Charron Frédéric, ;2010;The Journal of neuroscience : the official journal of the Society for Neuroscience;30;266-75; 20053908
- Sonic hedgehog expression in corticofugal projection neurons directs cortical microcircuit formation.;Harwell Corey C, Parker Philip R L, Gee Steven M, Okada Ami, McConnell Susan K, Kreitzer Anatol C, Kriegstein Arnold R, ;2012;Neuron;73;1116-26; 22445340
- Shh/Boc signaling is required for sustained generation of ipsilateral projecting ganglion cells in the mouse retina.;Sánchez-Arrones Luisa, Nieto-Lopez Francisco, Sánchez-Camacho Cristina, Carreres M Isabel, Herrera Eloisa, Okada Ami, Bovolenta Paola, ;2013;The Journal of neuroscience : the official journal of the Society for Neuroscience;33;8596-607; 23678105
- Boc modifies the spectrum of holoprosencephaly in the absence of Gas1 function.;Seppala Maisa, Xavier Guilherme M, Fan Chen-Ming, Cobourne Martyn T, ;2014;Biology open;3;728-40; 25063195
- Gas1 is a receptor for sonic hedgehog to repel enteric axons.;Jin Shiying, Martinelli David C, Zheng Xiaobin, Tessier-Lavigne Marc, Fan Chen-Ming, ;2015;Proceedings of the National Academy of Sciences of the United States of America;112;E73-80; 25535338
- Sonic Hedgehog Is a Remotely Produced Cue that Controls Axon Guidance Trans-axonally at a Midline Choice Point.;Peng Jimmy, Fabre Pierre J, Dolique Tiphaine, Swikert Shannon M, Kermasson Laëtitia, Shimogori Tomomi, Charron Frédéric, ;2018;Neuron;97;326-340.e4; 29346753