IMPC phenotypes (gene matching)
B6.129P2(CD1)-Boctm1Aok/Cnbc
| Status | Available to order |
| EMMA ID | EM:11898 |
| International strain name | B6.129P2(CD1)-Boctm1Aok/Cnbc |
| Alternative name | Boc- |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Boctm1Aok |
| Gene/Transgene symbol | Boc |
Information from provider
| Provider | Ami Okada |
| Provider affiliation | Department of Biological Sciences, Stanford University |
| Genetic information | Homologous recombination was used to insert a gene trap vector encoding a strong splice acceptor site, transmembrane domain and beta-geo followed by an IRES-PLAP upstream of the exon encoding the transmembrane domain. The allele proved to be severely hypomorphic. |
| Phenotypic information | Homozygous:Nervous system, axonal circuit alterationsHeterozygous:Nervous system, axonal circuit alterations |
| Breeding history | Mutation first introduced through E14 (ES Cell) 129P2/OlaHsd, then bred to CD1 and eventually backcrossed to C57BL/6J for more than 10 generations. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | homozygous C57BL/6J, wild-type C57BL/6J |
| Stage of embryos | 2-cell |
Disease and phenotype information
Literature references
- Boc is a receptor for sonic hedgehog in the guidance of commissural axons.;Okada Ami, Charron Frédéric, Morin Steves, Shin David S, Wong Karen, Fabre Pierre J, Tessier-Lavigne Marc, McConnell Susan K, ;2006;Nature;444;369-73; 17086203
- Secreted frizzled related proteins modulate pathfinding and fasciculation of mouse retina ganglion cell axons by direct and indirect mechanisms.;Marcos Séverine, Nieto-Lopez Francisco, Sandonìs Africa, Cardozo Marcos Julian, Di Marco Fabiana, Esteve Pilar, Bovolenta Paola, ;2015;The Journal of neuroscience : the official journal of the Society for Neuroscience;35;4729-40; 25788689
- Segregation of ipsilateral retinal ganglion cell axons at the optic chiasm requires the Shh receptor Boc.;Fabre Pierre J, Shimogori Tomomi, Charron Frédéric, ;2010;The Journal of neuroscience : the official journal of the Society for Neuroscience;30;266-75; 20053908
- Sonic hedgehog expression in corticofugal projection neurons directs cortical microcircuit formation.;Harwell Corey C, Parker Philip R L, Gee Steven M, Okada Ami, McConnell Susan K, Kreitzer Anatol C, Kriegstein Arnold R, ;2012;Neuron;73;1116-26; 22445340
- Shh/Boc signaling is required for sustained generation of ipsilateral projecting ganglion cells in the mouse retina.;Sánchez-Arrones Luisa, Nieto-Lopez Francisco, Sánchez-Camacho Cristina, Carreres M Isabel, Herrera Eloisa, Okada Ami, Bovolenta Paola, ;2013;The Journal of neuroscience : the official journal of the Society for Neuroscience;33;8596-607; 23678105
- Boc modifies the spectrum of holoprosencephaly in the absence of Gas1 function.;Seppala Maisa, Xavier Guilherme M, Fan Chen-Ming, Cobourne Martyn T, ;2014;Biology open;3;728-40; 25063195
- Gas1 is a receptor for sonic hedgehog to repel enteric axons.;Jin Shiying, Martinelli David C, Zheng Xiaobin, Tessier-Lavigne Marc, Fan Chen-Ming, ;2015;Proceedings of the National Academy of Sciences of the United States of America;112;E73-80; 25535338
- Sonic Hedgehog Is a Remotely Produced Cue that Controls Axon Guidance Trans-axonally at a Midline Choice Point.;Peng Jimmy, Fabre Pierre J, Dolique Tiphaine, Swikert Shannon M, Kermasson Laëtitia, Shimogori Tomomi, Charron Frédéric, ;2018;Neuron;97;326-340.e4; 29346753