C57BL/6N-A^tm1Brd Adcy3^{tm1a(EUCOMM)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:11881
International strain name	C57BL/6N-A^tm1Brd Adcy3^{tm1a(EUCOMM)Wtsi}/WtsiH
Alternative name	EPD0460_1_A03
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Adcy3^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Adcy3
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0460_1_A03. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (allele matching)

increased hematocrit / IMPC
decreased lean body mass / IMPC
increased hemoglobin content / IMPC
increased total body fat amount / IMPC
preweaning lethality, incomplete penetrance / IMPC

IMPC phenotypes (gene matching)

increased hemoglobin content / IMPC
increased hematocrit / IMPC
preweaning lethality, incomplete penetrance / IMPC
increased total body fat amount / IMPC
decreased lean body mass / IMPC

MGI phenotypes (gene matching)

impaired fertilization / MGI
abnormal olfactory bulb morphology / MGI
abnormal sensory neuron innervation pattern / MGI
obese / MGI
decreased body weight / MGI
hyperactivity / MGI
reduced male fertility / MGI
reduced female fertility / MGI
decreased litter size / MGI
abnormal olfactory system physiology / MGI
abnormal olfaction / MGI
abnormal fertility/fecundity / MGI
no abnormal phenotype detected / MGI
decreased susceptibility to hepatic steatosis / MGI
asthenozoospermia / MGI
decreased circulating insulin level / MGI
decreased circulating renin level / MGI
impaired sperm capacitation / MGI
anosmia / MGI
impaired acrosome reaction / MGI
increased oxygen consumption / MGI
renal/urinary system phenotype / MGI
homeostasis/metabolism phenotype / MGI
decreased percent body fat/body weight / MGI
decreased renal glomerular filtration rate / MGI
increased circulating creatinine level / MGI
decreased circulating glucose level / MGI
decreased susceptibility to diet-induced obesity / MGI
decreased circulating leptin level / MGI
impaired olfaction / MGI
decreased white fat cell size / MGI
decreased brown fat cell size / MGI
decreased total body fat amount / MGI
postnatal lethality, complete penetrance / MGI
postnatal lethality, incomplete penetrance / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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