Sprague Dawley rat Rno20_DelUmodl1-Prmt2/YahIcsOrl
| Status | Available to order |
| EMMA ID | EM:11811 |
| International strain name | Sprague Dawley rat Rno20_DelUmodl1-Prmt2/YahIcsOrl |
| Alternative name | Rno20_Del |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Rno20 |
| Gene/Transgene symbol | 5'-Umodl1; 3'-Prmt2 |
Information from provider
| Provider | Yann HERAULT |
| Provider affiliation | Translational Medicine and Neurogenetics, I.G.B.M.C. |
| Genetic information | Sprague Dawley rat. Deletion of the genetic interval between Umodl1 and Prmt2 genes using CRISPR/Cas9 engineering. This method allowed us to cut and delete all the DNA sequence from one allele of chromosome 20. We obtained a deletion of 3.6Mb. For the technic used on all our rat models see PMID: 28266534 |
| Phenotypic information | Homozygous:No generation of homozygousHeterozygous:Development of early cataract on 50% of heterozygous animals, after 3 months of age. |
| Breeding history | 1 backcross on Sprague Dawley background |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous rat Sprague-Dawley, wild-type rat Sprague-Dawley |
| Stage of embryos | 2-cell |
Literature references
- Efficient and rapid generation of large genomic variants in rats and mice using CRISMERE.;Birling Marie-Christine, Schaeffer Laurence, André Philippe, Lindner Loic, Maréchal Damien, Ayadi Abdel, Sorg Tania, Pavlovic Guillaume, Hérault Yann, ;2017;Scientific reports;7;43331; 28266534