B6.129-Tg(Flt3-cre)#Ccb/Ieg
| Status | Available to order |
| EMMA ID | EM:11790 |
| International strain name | B6.129-Tg(Flt3-cre)#Ccb/Ieg |
| Alternative name | Tg(Flt3-cre)#Ccb |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Flt3-cre)#Ccb |
| Gene/Transgene symbol | Tg(Flt3-cre)#Ccb |
Information from provider
| Provider | Conrad Bleul |
| Provider affiliation | Boehm, Max-Planck-Institute of Immunobiologie and Epigenetics |
| Additional owner | Dr., Thomas Boehm, Max-Planck-Institute of Immunobiologie and Epigenetics, Freiburg, Germany |
| Genetic information | A sequence encoding cre recombinase together with a kanamycin cassette flanked by frt sites was introduced into bacterial artificial chromosome (BAC) RP24-145C8 (BACPAC Resources Center) by ET cloning according to the manufacturer’s recommendations (Gene Bridges). In a deviation from the protocol, negative selection was performed by selecting for kanamycin-sensitive colonies after transfecting the Flp recombinase–containing plasmid 706Flp:tet (Gene Bridges). |
| Phenotypic information | Homozygous:Not applicableHeterozygous:Heterozygous/hemizygous mice show no special phenotype but look like their wild-type littermates. Transgenes only occur in male mice and most of the times in all males of the litter. |
| Breeding history | Founders mated to 129X1/SvJ for some generations, then backcrossed to C57BL/6J for more than 5 generations. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Veterinary Medicine, Vienna, Austria |
| Animals used for archiving | heterozygous 129 x C57BL/6 |
Literature references
- The stream of precursors that colonizes the thymus proceeds selectively through the early T lineage precursor stage of T cell development.;Benz Claudia, Martins Vera C, Radtke Freddy, Bleul Conrad C, ;2008;The Journal of experimental medicine;205;1187-99; 18458114
- Tissue-resident macrophages originate from yolk-sac-derived erythro-myeloid progenitors.;Gomez Perdiguero Elisa, Klapproth Kay, Schulz Christian, Busch Katrin, Azzoni Emanuele, Crozet Lucile, Garner Hannah, Trouillet Celine, de Bruijn Marella F, Geissmann Frederic, Rodewald Hans-Reimer, ;2015;Nature;518;547-51; 25470051
- A Transient Developmental Hematopoietic Stem Cell Gives Rise to Innate-like B and T Cells.;Beaudin Anna E, Boyer Scott W, Perez-Cunningham Jessica, Hernandez Gloria E, Derderian S Christopher, Jujjavarapu Chethan, Aaserude Eric, MacKenzie Tippi, Forsberg E Camilla, ;2016;Cell stem cell;19;768-783; 27666010