B6.129-Tg(Flt3-cre)#Ccb/Ieg
| Status | Available to order |
| EMMA ID | EM:11790 |
| Citation information | RRID:IMSR_EM:11790 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129-Tg(Flt3-cre)#Ccb/Ieg |
| Alternative name | Tg(Flt3-cre)#Ccb |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Flt3-cre)#Ccb |
| Gene/Transgene symbol | Tg(Flt3-cre)#Ccb |
Information from provider
| Provider | Conrad Bleul |
| Provider affiliation | Boehm, Max-Planck-Institute of Immunobiologie and Epigenetics |
| Additional owner | Dr., Thomas Boehm, Max-Planck-Institute of Immunobiologie and Epigenetics, Freiburg, Germany |
| Genetic information | A sequence encoding cre recombinase together with a kanamycin cassette flanked by frt sites was introduced into bacterial artificial chromosome (BAC) RP24-145C8 (BACPAC Resources Center) by ET cloning according to the manufacturer’s recommendations (Gene Bridges). In a deviation from the protocol, negative selection was performed by selecting for kanamycin-sensitive colonies after transfecting the Flp recombinase–containing plasmid 706Flp:tet (Gene Bridges). |
| Phenotypic information | Homozygous:Not applicableHeterozygous:Heterozygous/hemizygous mice show no special phenotype but look like their wild-type littermates. Transgenes only occur in male mice and most of the times in all males of the litter. |
| Breeding history | Founders mated to 129X1/SvJ for some generations, then backcrossed to C57BL/6J for more than 5 generations. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Veterinary Medicine, Vienna, Austria |
| Animals used for archiving | heterozygous 129 x C57BL/6 males |
Literature references
- The stream of precursors that colonizes the thymus proceeds selectively through the early T lineage precursor stage of T cell development.;Benz Claudia, Martins Vera C, Radtke Freddy, Bleul Conrad C, ;2008;The Journal of experimental medicine;205;1187-99; 18458114
- Tissue-resident macrophages originate from yolk-sac-derived erythro-myeloid progenitors.;Gomez Perdiguero Elisa, Klapproth Kay, Schulz Christian, Busch Katrin, Azzoni Emanuele, Crozet Lucile, Garner Hannah, Trouillet Celine, de Bruijn Marella F, Geissmann Frederic, Rodewald Hans-Reimer, ;2015;Nature;518;547-51; 25470051
- A Transient Developmental Hematopoietic Stem Cell Gives Rise to Innate-like B and T Cells.;Beaudin Anna E, Boyer Scott W, Perez-Cunningham Jessica, Hernandez Gloria E, Derderian S Christopher, Jujjavarapu Chethan, Aaserude Eric, MacKenzie Tippi, Forsberg E Camilla, ;2016;Cell stem cell;19;768-783; 27666010