IMPC phenotypes (gene matching)
B6.129-Unc13btm1Rmnd/Cnrm
| Status | Available to order |
| EMMA ID | EM:11739 |
| Citation information | RRID:IMSR_EM:11739 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129-Unc13btm1Rmnd/Cnrm |
| Alternative name | Unc13b tm1Rmnd |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Unc13btm1Rmnd |
| Gene/Transgene symbol | Unc13b |
Information from provider
| Provider | Kerstin Reim |
| Provider affiliation | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine |
| Genetic information | In the targeting vector, a 6-kb genomic fragment containing multiple exons of the Munc13-2 gene that are shared by the two Munc13-2 splice variants [bp 2907–3904 of bMunc13-2 cDNA (GenBank U24071); bp 1439–2436 of ub-Munc13-2 cDNA (GenBank AF159706)] was replaced by a neomycin resistance cassette. Immunoblotting of adult brain homogenates with an antibody recognizing both Munc13-2 splice variants demonstrated the total absence of Munc13-2 in mutant brains. |
| Phenotypic information | Homozygous:Apart from sporadic seizures in older animals (above 12 months of age), Munc13-2 KOs were indistinguishable from wild-type littermates.Heterozygous:Heterozygous mice are normal and show no obvious alterations in comparison to wild-type controls. |
| Breeding history | Homologous recombination in embryonic stem cells of 129/Sv, consecutive backcrosses with C57BL/6J animals. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
Literature references
- Total arrest of spontaneous and evoked synaptic transmission but normal synaptogenesis in the absence of Munc13-mediated vesicle priming.;Varoqueaux Frederique, Sigler Albrecht, Rhee Jeong-Seop, Brose Nils, Enk Carsten, Reim Kerstin, Rosenmund Christian, ;2002;Proceedings of the National Academy of Sciences of the United States of America;99;9037-42; 12070347