IMPC phenotypes (gene matching)
B6.129-Unc13btm1Rmnd/Cnrm
| Status | Available to order |
| EMMA ID | EM:11739 |
| International strain name | B6.129-Unc13btm1Rmnd/Cnrm |
| Alternative name | Unc13b tm1Rmnd |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Unc13btm1Rmnd |
| Gene/Transgene symbol | Unc13b |
Information from provider
| Provider | Kerstin Reim |
| Provider affiliation | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine |
| Genetic information | In the targeting vector, a 6-kb genomic fragment containing multiple exons of the Munc13-2 gene that are shared by the two Munc13-2 splice variants [bp 2907–3904 of bMunc13-2 cDNA (GenBank U24071); bp 1439–2436 of ub-Munc13-2 cDNA (GenBank AF159706)] was replaced by a neomycin resistance cassette. Immunoblotting of adult brain homogenates with an antibody recognizing both Munc13-2 splice variants demonstrated the total absence of Munc13-2 in mutant brains. |
| Phenotypic information | Homozygous:Apart from sporadic seizures in older animals (above 12 months of age), Munc13-2 KOs were indistinguishable from wild-type littermates.Heterozygous:Heterozygous mice are normal and show no obvious alterations in comparison to wild-type controls. |
| Breeding history | Homologous recombination in embryonic stem cells of 129/Sv, consecutive backcrosses with C57BL/6J animals. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
Literature references
- Total arrest of spontaneous and evoked synaptic transmission but normal synaptogenesis in the absence of Munc13-mediated vesicle priming.;Varoqueaux Frederique, Sigler Albrecht, Rhee Jeong-Seop, Brose Nils, Enk Carsten, Reim Kerstin, Rosenmund Christian, ;2002;Proceedings of the National Academy of Sciences of the United States of America;99;9037-42; 12070347