B6;129P2-Rhd^tm1Goo Rhag^tm1Goo/Orl

Status	Available to order
EMMA ID	EM:11708
International strain name	B6;129P2-Rhd^tm1Goo Rhag^tm1Goo/Orl
Alternative name	GOO1 x GOO2 also Rhdtm1GOOxRhagtm1GOO (MGI:5318588x5318589)
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Rhd^tm1Goo, Rhag^tm1Goo
Gene/Transgene symbol	Rhd, Rhag

Information from provider

Provider	Dominique GOOSSENS
Provider affiliation	UMRS_1134-Inserm-Dept de recherches biologiques sur le globule rouge, Institut National de la Transfusion Sanguine- Inserm
Genetic information	Rhd and Rhag (erythrocyte membrane proteins) gene knockout cross (double knockout).
Phenotypic information	Homozygous: No clinical abnormalities, complete loss of Rhd and Rhag and mouse RH erythrocyte complex proteins, disruption of erythrocyte ammonium transport. Heterozygous: normal
References	Generation and characterisation of Rhd and Rhag null mice.;Goossens Dominique, Trinh-Trang-Tan Marie-Marcelle, Debbia Martine, Ripoche Pierre, Vilela-Lamego Camilo, Louache Fawzia, Vainchenker William, Colin Yves, Cartron Jean-Pierre, ;2010;British journal of haematology;148;161-72; 19807729 Generation of mice with inactivated Rh or Rhag genes.;Goossens D, Bony V, Gane P, Colin Y, Cartron J-P, ;2006;Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine;13;164-6; 16581281 Role of RhAG and AQP1 in NH3 and CO2 gas transport in red cell ghosts: a stopped-flow analysis.;Ripoche P, Goossens D, Devuyst O, Gane P, Colin Y, Verkman A S, Cartron J-P, ;2006;Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine;13;117-22; 16574458
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	yes
Immunocompromised	no

Information from EMMA

Archiving centre	Institut de Transgenose, INTRAGENE, Orléans, France
Animals used for archiving	homozygous 129 x C57BL/6, homozygous 129 x C57BL/6
Stage of embryos	2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Overhydrated hereditary stomatocytosis / Orphanet_3203
Rh deficiency syndrome / Orphanet_71275

IMPC phenotypes (gene matching)

abnormal thymus morphology / IMPC
increased circulating cholesterol level / IMPC
increased exploration in new environment / IMPC
decreased mean corpuscular hemoglobin / IMPC
decreased hemoglobin content / IMPC
enlarged lymph nodes / IMPC
decreased mean corpuscular hemoglobin concentration / IMPC
decreased mean corpuscular hemoglobin / IMPC
abnormal lymph node morphology / IMPC
decreased mean corpuscular volume / IMPC
decreased circulating cholesterol level / IMPC
abnormal skin condition / IMPC
small testis / IMPC
abnormal testis morphology / IMPC
increased circulating total protein level / IMPC
increased circulating HDL cholesterol level / IMPC
decreased circulating free fatty acids level / IMPC
enlarged heart / IMPC
decreased circulating alkaline phosphatase level / IMPC
decreased circulating HDL cholesterol level / IMPC
abnormal heart morphology / IMPC
enlarged thymus / IMPC

MGI phenotypes (gene matching)

abnormal erythrocyte physiology / MGI
increased circulating iron level / MGI
abnormal blood homeostasis / MGI
decreased mean corpuscular volume / MGI
decreased hemoglobin content / MGI
abnormal erythrocyte physiology / MGI
decreased mean corpuscular hemoglobin concentration / MGI
increased circulating iron level / MGI

Literature references

Generation and characterisation of Rhd and Rhag null mice.;Goossens Dominique, Trinh-Trang-Tan Marie-Marcelle, Debbia Martine, Ripoche Pierre, Vilela-Lamego Camilo, Louache Fawzia, Vainchenker William, Colin Yves, Cartron Jean-Pierre, ;2010;British journal of haematology;148;161-72; 19807729
Generation of mice with inactivated Rh or Rhag genes.;Goossens D, Bony V, Gane P, Colin Y, Cartron J-P, ;2006;Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine;13;164-6; 16581281
Role of RhAG and AQP1 in NH3 and CO2 gas transport in red cell ghosts: a stopped-flow analysis.;Ripoche P, Goossens D, Devuyst O, Gane P, Colin Y, Verkman A S, Cartron J-P, ;2006;Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine;13;117-22; 16574458

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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B6;129P2-Rhdtm1Goo Rhagtm1Goo/Orl