C57BL/6NCrl-Tyk2^{tm2b(EUCOMM)Hmgu}/Ieg

Status	Available to order
EMMA ID	EM:11687
International strain name	C57BL/6NCrl-Tyk2^{tm2b(EUCOMM)Hmgu}/Ieg
Alternative name	HEPD0809_8_G11
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Tyk2^{tm2b(EUCOMM)Hmgu}
Gene/Transgene symbol	Tyk2
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Provider affiliation	Institute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic information	This mouse line originates from EUCOMM ES clone HEPD0809_8_G11. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele. Click here for more information on EUCOMM final vectors.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	heterozygous C57BL/6N

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Susceptibility to infection due to TYK2 deficiency / Orphanet_331226

IMPC phenotypes (allele matching)

enlarged spleen / IMPC
enlarged thymus / IMPC
abnormal ovary morphology / IMPC
abnormal pancreas morphology / IMPC
abnormal seminal vesicle morphology / IMPC
decreased plasmacytoid dendritic cell number / IMPC
decreased heart left ventricle size / IMPC

IMPC phenotypes (gene matching)

enlarged thymus / IMPC
abnormal seminal vesicle morphology / IMPC
decreased plasmacytoid dendritic cell number / IMPC
abnormal pancreas morphology / IMPC
decreased heart left ventricle size / IMPC
abnormal ovary morphology / IMPC
enlarged spleen / IMPC

MGI phenotypes (gene matching)

increased body weight / MGI
obese / MGI
increased circulating free fatty acid level / MGI
abnormal body temperature homeostasis / MGI
decreased IgG level / MGI
increased circulating insulin level / MGI
abnormal T cell differentiation / MGI
increased susceptibility to viral infection / MGI
abnormal T cell physiology / MGI
abnormal brown adipose tissue morphology / MGI
abnormal cell cycle / MGI
decreased susceptibility to induced arthritis / MGI
abnormal nitric oxide homeostasis / MGI
increased cholesterol level / MGI
decreased energy expenditure / MGI
abnormal response to infection / MGI
increased susceptibility to parasitic infection / MGI
impaired glucose tolerance / MGI
abnormal T-helper 1 physiology / MGI
abnormal mitochondrial physiology / MGI
abnormal common myeloid progenitor cell morphology / MGI
decreased IgG1 level / MGI
decreased IgG2a level / MGI
decreased interferon-gamma secretion / MGI
increased circulating interleukin-12 level / MGI
abnormal splenocyte physiology / MGI
increased susceptibility to viral infection induced morbidity/mortality / MGI
impaired adaptive thermogenesis / MGI
decreased macrophage nitric oxide production / MGI
abnormal fat cell differentiation / MGI
disorganized mitochondrial cristae / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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