C57BL/6N-Nsun2tm1c(EUCOMM)Wtsi/WtsiOulu

Status

Available to order

EMMA IDEM:11650
Citation informationRRID:IMSR_EM:11650 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameC57BL/6N-Nsun2tm1c(EUCOMM)Wtsi/WtsiOulu
Alternative nameEPD0105_2_F10
Strain typeTargeted Mutant Strains : Targeted Conditional
Allele/Transgene symbolNsun2tm1c(EUCOMM)Wtsi
Gene/Transgene symbolNsun2
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis line originates from EUCOMM ES clone EPD0105_2_F10, after breeding with a Flp recombinase deleter line to convert the original targeted allele tm1a (knock-out first allele) into a conditional allele tm1c. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreUniversity of Oulu, Oulu, Finland

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (gene matching)
  • abnormal snout morphology / IMPC
  • abnormal gait / IMPC
  • hypoplasia / IMPC
  • process of degenerative change / IMPC
  • abnormal eye size / IMPC
  • decreased body weight / IMPC
  • abnormal humerus morphology / IMPC
  • excessive tearing / IMPC
  • abnormal joint morphology / IMPC
  • synechia / IMPC
  • preweaning lethality, incomplete penetrance / IMPC
  • abnormal cornea morphology / IMPC
  • developmental and structural abnormality / IMPC
  • abnormal response to new environment / IMPC
  • abnormal cranium morphology / IMPC
  • abnormal tooth morphology / IMPC
MGI phenotypes (gene matching)
  • decreased bone mineral density / MGI
  • abnormal rib morphology / MGI
  • kyphosis / MGI
  • lordosis / MGI
  • decreased circulating LDL cholesterol level / MGI
  • decreased circulating HDL cholesterol level / MGI
  • alopecia / MGI
  • abnormal hair cycle / MGI
  • abnormal cranium morphology / MGI
  • abnormal snout morphology / MGI
  • abnormal liver physiology / MGI
  • abnormal forebrain morphology / MGI
  • abnormal dorsal root ganglion morphology / MGI
  • small superior cervical ganglion / MGI
  • abnormal testis morphology / MGI
  • abnormal epidermis stratum basale morphology / MGI
  • decreased body length / MGI
  • decreased body weight / MGI
  • decreased body size / MGI
  • abnormal lens morphology / MGI
  • cataract / MGI
  • abnormal cornea morphology / MGI
  • hyperactivity / MGI
  • male infertility / MGI
  • abnormal tooth morphology / MGI
  • abnormal fertility/fecundity / MGI
  • abnormal thymus capsule morphology / MGI
  • decreased mean corpuscular volume / MGI
  • oligozoospermia / MGI
  • decreased circulating free fatty acid level / MGI
  • abnormal joint morphology / MGI
  • increased erythrocyte cell number / MGI
  • decreased circulating glycerol level / MGI
  • abnormal eye muscle morphology / MGI
  • decreased lean body mass / MGI
  • retroesophageal right subclavian artery / MGI
  • abnormal spine curvature / MGI
  • abnormal optic stalk morphology / MGI
  • abnormal optic cup morphology / MGI
  • abnormal deltoid tuberosity morphology / MGI
  • basisphenoid bone foramen / MGI
  • fusion of vertebral arches / MGI
  • absent stapedial artery / MGI
  • decreased length of long bones / MGI
  • increased energy expenditure / MGI
  • decreased energy expenditure / MGI
  • decreased circulating cholesterol level / MGI
  • increased oxygen consumption / MGI
  • improved glucose tolerance / MGI
  • abnormal humerus morphology / MGI
  • decreased percent body fat/body weight / MGI
  • decreased circulating glucose level / MGI
  • decreased mean corpuscular hemoglobin / MGI
  • decreased circulating amylase level / MGI
  • abnormal hair cycle anagen phase / MGI
  • abnormal hair shedding / MGI
  • thoracoschisis / MGI
  • increased carbon dioxide production / MGI
  • abnormal optic chiasm morphology / MGI
  • decreased total body fat amount / MGI
  • decreased grip strength / MGI
  • decreased bone mineral content / MGI
  • lethality, incomplete penetrance / MGI
  • abnormal systemic artery morphology / MGI
  • testis degeneration / MGI
  • absent hypoglossal canal / MGI
  • thin hypoglossal nerve / MGI
  • absent hypoglossal nerve / MGI
  • abnormal hypoglossal nerve topology / MGI
  • absent segment of posterior cerebral artery / MGI
  • ductus venosus stenosis / MGI
  • subcutaneous edema / MGI
  • trigeminal neuroma / MGI
  • absent ductus venosus valve / MGI
  • abnormal ductus venosus valve morphology / MGI
  • thin motoric part of trigeminal nerve / MGI
  • intraembryonal intestine elongation / MGI
  • abnormal infrahyoid muscle connection / MGI
  • reduced sympathetic cervical ganglion size / MGI
  • persistent dorsal ophthalmic artery / MGI
  • abnormal external carotid artery origin / MGI
  • abnormal vertebral artery topology / MGI
  • persistent trigeminal artery / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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