C57BL/6N-A^tm1Brd Hbs1l^{tm1a(KOMP)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:11622
International strain name	C57BL/6N-A^tm1Brd Hbs1l^{tm1a(KOMP)Wtsi}/WtsiH
Alternative name	EPD0693_2_A02
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Hbs1l^{tm1a(KOMP)Wtsi}
Gene/Transgene symbol	Hbs1l
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from KOMP ES clone EPD0693_2_A02. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (allele matching)

increased leukocyte cell number / IMPC
decreased monocyte cell number / IMPC
abnormal cranium morphology / IMPC
abnormal snout morphology / IMPC
corneal opacity / IMPC
abnormal retina morphology / IMPC
increased circulating free fatty acids level / IMPC
increased circulating HDL cholesterol level / IMPC
male infertility / IMPC
abnormal tail morphology / IMPC
increased mean platelet volume / IMPC
increased circulating alkaline phosphatase level / IMPC
vertebral transformation / IMPC
abnormal bone structure / IMPC
increased regulatory T cell number / IMPC
increased circulating cholesterol level / IMPC
improved glucose tolerance / IMPC
thrombocytosis / IMPC
increased mean corpuscular hemoglobin / IMPC
increased circulating total protein level / IMPC
decreased Langerhans cell number / IMPC
decreased mature B cell number / IMPC
increased circulating iron level / IMPC
decreased total body fat amount / IMPC
decreased bone mineral content / IMPC
increased CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
increased CD4-positive, alpha-beta memory T cell number / IMPC
increased effector memory CD8-positive, alpha-beta T cell number / IMPC
decreased effector memory CD8-positive, alpha-beta T cell number / IMPC
increased CD5-positive gamma-delta T cell number / IMPC
increased memory CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
decreased memory-marker CD4-negative NK T cell number / IMPC
decreased T-helper cell number / IMPC

IMPC phenotypes (gene matching)

abnormal retina morphology / IMPC
decreased total body fat amount / IMPC
increased circulating free fatty acids level / IMPC
decreased effector memory CD8-positive, alpha-beta T cell number / IMPC
decreased monocyte cell number / IMPC
increased regulatory T cell number / IMPC
increased effector memory CD8-positive, alpha-beta T cell number / IMPC
increased mean platelet volume / IMPC
thrombocytosis / IMPC
decreased memory-marker CD4-negative NK T cell number / IMPC
increased CD4-positive, alpha-beta memory T cell number / IMPC
abnormal snout morphology / IMPC
increased CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
male infertility / IMPC
increased memory CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
abnormal tail morphology / IMPC
decreased mature B cell number / IMPC
increased circulating total protein level / IMPC
increased mean corpuscular hemoglobin / IMPC
decreased T-helper cell number / IMPC
improved glucose tolerance / IMPC
decreased bone mineral content / IMPC
abnormal cranium morphology / IMPC
increased circulating HDL cholesterol level / IMPC
increased CD5-positive gamma-delta T cell number / IMPC
increased circulating cholesterol level / IMPC
increased circulating iron level / IMPC
abnormal bone structure / IMPC
decreased Langerhans cell number / IMPC
increased circulating alkaline phosphatase level / IMPC
vertebral transformation / IMPC
increased leukocyte cell number / IMPC
corneal opacity / IMPC

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Atm1Brd Hbs1ltm1a(KOMP)Wtsi/WtsiH