C57BL/6NCrl-Nub1tm1b(EUCOMM)Hmgu/Ph

Status

Available to order

EMMA IDEM:11613
International strain nameC57BL/6NCrl-Nub1tm1b(EUCOMM)Hmgu/Ph
Alternative nameHEPD0664_6_C08
Strain typeTargeted Mutant Strains
Allele/Transgene symbolNub1tm1b(EUCOMM)Hmgu
Gene/Transgene symbolNub1
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Institute of Molecular Genetics
Provider affiliationDepartment of Transgenic Models of Diseases, Institute of Molecular Genetics
Genetic informationThis mouse line originates from EUCOMM ES clone HEPD0664_6_C08. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele (Gt(ROSA)26Sortm1(ACTB-cre,-EGFP)Ics (MGI:5285392)). Click here for more information on EUCOMM final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreInstitute of Molecular Genetics, Prague, Czech Republic
Animals used for archivingheterozygous C57BL/6NCrl

Disease and phenotype information

IMPC phenotypes (allele matching)
  • abnormal heart morphology / IMPC
  • abnormal spleen morphology / IMPC
  • enlarged spleen / IMPC
  • abnormal lens morphology / IMPC
  • abnormal retina morphology / IMPC
  • abnormal retina vasculature morphology / IMPC
  • decreased circulating iron level / IMPC
  • immune system phenotype / IMPC
  • increased circulating potassium level / IMPC
  • decreased NK T cell number / IMPC
  • decreased NK cell number / IMPC
  • decreased CD4-positive, alpha-beta T cell number / IMPC
  • decreased CD8-positive, alpha-beta T cell number / IMPC
  • abnormal optic disk morphology / IMPC
  • increased heart atrium size / IMPC
  • abnormal retina blood vessel morphology / IMPC
  • increased effector memory CD8-positive, alpha-beta T cell number / IMPC
  • decreased CD4-negative NK T cell number / IMPC
  • decreased memory-marker CD4-negative NK T cell number / IMPC
  • decreased CD11b-high dendritic cell number / IMPC
  • decreased Ly6C-positive NK T cell number / IMPC
  • increased Ly6C-positive NK T cell number / IMPC
  • decreased CD5-positive Ly6C-positive T cell number / IMPC
  • increased effector memory T-helper cell number / IMPC
  • increased freezing behavior / IMPC
IMPC phenotypes (gene matching)
  • abnormal optic disk morphology / IMPC
  • decreased CD4-positive, alpha-beta T cell number / IMPC
  • increased freezing behavior / IMPC
  • abnormal spleen morphology / IMPC
  • immune system phenotype / IMPC
  • decreased Ly6C-positive NK T cell number / IMPC
  • decreased circulating iron level / IMPC
  • increased heart atrium size / IMPC
  • abnormal retina morphology / IMPC
  • abnormal retina blood vessel morphology / IMPC
  • increased Ly6C-positive NK T cell number / IMPC
  • decreased memory-marker CD4-negative NK T cell number / IMPC
  • abnormal retina vasculature morphology / IMPC
  • decreased NK T cell number / IMPC
  • decreased CD4-negative NK T cell number / IMPC
  • abnormal lens morphology / IMPC
  • enlarged spleen / IMPC
  • decreased CD5-positive Ly6C-positive T cell number / IMPC
  • increased effector memory T-helper cell number / IMPC
  • abnormal heart morphology / IMPC
  • increased circulating potassium level / IMPC
  • decreased CD8-positive, alpha-beta T cell number / IMPC
  • increased effector memory CD8-positive, alpha-beta T cell number / IMPC
  • decreased NK cell number / IMPC
  • decreased CD11b-high dendritic cell number / IMPC

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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