B6.Cg-Lgi1^tm1.1Ics/Orl

Status	Available to order
EMMA ID	EM:11505
International strain name	B6.Cg-Lgi1^tm1.1Ics/Orl
Alternative name	Lgi1 KO
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Lgi1^tm1.1Ics
Gene/Transgene symbol	Lgi1

Information from provider

Provider	Stephanie Baulac
Provider affiliation	Hopital PItié-Salpetriere, Institut du Cerveau et de la Moelle-ICM
Genetic information	Knockout of the Lgi1 gene.
Phenotypic information	Homozygous: Homozygous mice do not survive after postnatal day 20. At postnatal day 10, first epileptic seizures occur. At postnatal day 14, mice are inactive. Heterozygous: Heterozygous mice have a lower threshold for audiogenic induced seizures.
Breeding history	The substrain used for backcrossing is C57BL/6J.
References	Electroclinical characterization of epileptic seizures in leucine-rich, glioma-inactivated 1-deficient mice.;Chabrol Elodie, Navarro Vincent, Provenzano Giovanni, Cohen Ivan, Dinocourt Céline, Rivaud-Péchoux Sophie, Fricker Desdemona, Baulac Michel, Miles Richard, Leguern Eric, Baulac Stéphanie, ;2010;Brain : a journal of neurology;133;2749-62; 20659958 LGI1 acts presynaptically to regulate excitatory synaptic transmission during early postnatal development.;Boillot Morgane, Lee Chun-Yao, Allene Camille, Leguern Eric, Baulac Stéphanie, Rouach Nathalie, ;2016;Scientific reports;6;21769; 26878798 Epilepsy gene LGI1 regulates postnatal developmental remodeling of retinogeniculate synapses.;Zhou Yu-Dong, Zhang Dawei, Ozkaynak Ekim, Wang Xuan, Kasper Ekkehard M, Leguern Eric, Baulac Stéphanie, Anderson Matthew P, ;2012;The Journal of neuroscience : the official journal of the Society for Neuroscience;32;903-10; 22262888
Homozygous fertile	no
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Institut de Transgenose, INTRAGENE, Orléans, France
Animals used for archiving	heterozygous C57BL/6J

Disease and phenotype information

MGI allele-associated human disease models

familial temporal lobe epilepsy 1 / DOID:0060748

Orphanet associated rare diseases, based on orthologous gene matching

Autosomal dominant epilepsy with auditory features / Orphanet_101046

MGI phenotypes (allele matching)

audiogenic seizures / MGI
abnormal cerebellum external granule cell layer morphology / MGI
decreased body weight / MGI
decreased body size / MGI
aphagia / MGI
postnatal growth retardation / MGI
seizures / MGI
gliosis / MGI
neurodegeneration / MGI
akinesia / MGI
abnormal hippocampal mossy fiber morphology / MGI
tonic-clonic seizures / MGI
abnormal cerebellar granule cell morphology / MGI
abnormal brain wave pattern / MGI
abnormal spike wave discharge / MGI
environmentally induced seizures / MGI
decreased total body fat amount / MGI
postnatal lethality, complete penetrance / MGI

MGI phenotypes (gene matching)

abnormal cerebellum external granule cell layer morphology / MGI
decreased body weight / MGI
decreased body size / MGI
aphagia / MGI
audiogenic seizures / MGI
postnatal growth retardation / MGI
seizures / MGI
premature death / MGI
gliosis / MGI
neurodegeneration / MGI
akinesia / MGI
abnormal hippocampal mossy fiber morphology / MGI
tonic seizures / MGI
abnormal glutamate-mediated receptor currents / MGI
increased susceptibility to pharmacologically induced seizures / MGI
nervous system phenotype / MGI
clonic seizures / MGI
tonic-clonic seizures / MGI
abnormal cerebellar granule cell morphology / MGI
abnormal miniature excitatory postsynaptic currents / MGI
abnormal brain wave pattern / MGI
abnormal spike wave discharge / MGI
environmentally induced seizures / MGI
decreased total body fat amount / MGI
postnatal lethality, complete penetrance / MGI

Literature references

Electroclinical characterization of epileptic seizures in leucine-rich, glioma-inactivated 1-deficient mice.;Chabrol Elodie, Navarro Vincent, Provenzano Giovanni, Cohen Ivan, Dinocourt Céline, Rivaud-Péchoux Sophie, Fricker Desdemona, Baulac Michel, Miles Richard, Leguern Eric, Baulac Stéphanie, ;2010;Brain : a journal of neurology;133;2749-62; 20659958
LGI1 acts presynaptically to regulate excitatory synaptic transmission during early postnatal development.;Boillot Morgane, Lee Chun-Yao, Allene Camille, Leguern Eric, Baulac Stéphanie, Rouach Nathalie, ;2016;Scientific reports;6;21769; 26878798
Epilepsy gene LGI1 regulates postnatal developmental remodeling of retinogeniculate synapses.;Zhou Yu-Dong, Zhang Dawei, Ozkaynak Ekim, Wang Xuan, Kasper Ekkehard M, Leguern Eric, Baulac Stéphanie, Anderson Matthew P, ;2012;The Journal of neuroscience : the official journal of the Society for Neuroscience;32;903-10; 22262888

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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B6.Cg-Lgi1tm1.1Ics/Orl