B6.129P2-Pik3cd^tm1Bvan/IcsOrl

Status	Available to order
EMMA ID	EM:11501
International strain name	B6.129P2-Pik3cd^tm1Bvan/IcsOrl
Alternative name	p110delta PI3K WT-Myc-IRES-LacZ
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Pik3cd^tm1Bvan
Gene/Transgene symbol	Pik3cd

Information from provider

Provider	Bart Vanhaesebroeck
Provider affiliation	UCL Cancer Institute, University College London
Additional owner	Co-developed by the Ludwig Institute for Cancer Research (LICR) and the University of Edinburgh, UK. The materials are available to academic researchers. Commercial entity need to obtain a licensing agreement.
Genetic information	Introduced by homologous recombination in the Pik3cd locus: Myc tag added to the C-terminus of the p110delta protein plus IRES-lacZ-MC1-neo marker/selection cassette, flanked by loxP sites
Phenotypic information	Homozygous: Not characterised as homozygous mice Heterozygous: Mice are expected to have an inactivated p110delta due to the presence of C-terminal Myc-tag and express lacZ reporter where p110delta is expressed
Breeding history	Backcrossed onto C57BL/6J for more than 10 generations
References	Impaired B and T cell antigen receptor signaling in p110delta PI 3-kinase mutant mice.;Okkenhaug Klaus, Bilancio Antonio, Farjot Géraldine, Priddle Helen, Sancho Sara, Peskett Emma, Pearce Wayne, Meek Stephen E, Salpekar Ashreena, Waterfield Michael D, Smith Andrew J H, Vanhaesebroeck Bart, ;2002;Science (New York, N.Y.);297;1031-4; 12130661 Control of axonal growth and regeneration of sensory neurons by the p110delta PI 3-kinase.;Eickholt Britta J, Ahmed Aminul I, Davies Meirion, Papakonstanti Evangelia A, Pearce Wayne, Starkey Michelle L, Bilancio Antonio, Need Anna C, Smith Andrew J H, Hall Susan M, Hamers Frank P, Giese Karl P, Bradbury Elizabeth J, Vanhaesebroeck Bart, ;2007;PloS one;2;e869; 17846664
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Institut de Transgenose, INTRAGENE, Orléans, France

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Activated PI3K-delta syndrome / Orphanet_397596
Combined immunodeficiency with faciooculoskeletal anomalies / Orphanet_221139

MGI phenotypes (allele matching)

no phenotypic analysis / MGI

MGI phenotypes (gene matching)

increased leukocyte cell number / MGI
increased neutrophil cell number / MGI
abnormal intestinal epithelium morphology / MGI
crypts of Lieberkuhn abscesses / MGI
abnormal intestinal mucosa morphology / MGI
small spleen / MGI
spleen hypoplasia / MGI
impaired hematopoiesis / MGI
abnormal humoral immune response / MGI
decreased IgG level / MGI
decreased IgM level / MGI
decreased IgA level / MGI
intestinal inflammation / MGI
decreased inflammatory response / MGI
abnormal B cell differentiation / MGI
small lymph nodes / MGI
abnormal Peyer's patch germinal center morphology / MGI
increased susceptibility to viral infection / MGI
abnormal B cell physiology / MGI
decreased immunoglobulin level / MGI
increased IgE level / MGI
no phenotypic analysis / MGI
decreased susceptibility to induced arthritis / MGI
decreased B-1 B cell number / MGI
increased lymphocyte cell number / MGI
decreased B cell number / MGI
decreased B cell proliferation / MGI
cardiovascular system phenotype / MGI
immune system phenotype / MGI
decreased B-1a cell number / MGI
decreased marginal zone B cell number / MGI
absent B-1 B cells / MGI
decreased B-2 B cell number / MGI
decreased mature B cell number / MGI
absent spleen germinal center / MGI
decreased IgG1 level / MGI
decreased IgG2a level / MGI
decreased IgG2b level / MGI
decreased IgG3 level / MGI
increased circulating interferon-alpha level / MGI
abnormal platelet aggregation / MGI
decreased response to antigen / MGI
increased response to antigen / MGI

Literature references

Impaired B and T cell antigen receptor signaling in p110delta PI 3-kinase mutant mice.;Okkenhaug Klaus, Bilancio Antonio, Farjot Géraldine, Priddle Helen, Sancho Sara, Peskett Emma, Pearce Wayne, Meek Stephen E, Salpekar Ashreena, Waterfield Michael D, Smith Andrew J H, Vanhaesebroeck Bart, ;2002;Science (New York, N.Y.);297;1031-4; 12130661
Control of axonal growth and regeneration of sensory neurons by the p110delta PI 3-kinase.;Eickholt Britta J, Ahmed Aminul I, Davies Meirion, Papakonstanti Evangelia A, Pearce Wayne, Starkey Michelle L, Bilancio Antonio, Need Anna C, Smith Andrew J H, Hall Susan M, Hamers Frank P, Giese Karl P, Bradbury Elizabeth J, Vanhaesebroeck Bart, ;2007;PloS one;2;e869; 17846664

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6.129P2-Pik3cdtm1Bvan/IcsOrl