C57BL/6NTac-Cir1^{tm3a(KOMP)Wtsi}/WtsiIeg

Status	Available to order
EMMA ID	EM:11497
International strain name	C57BL/6NTac-Cir1^{tm3a(KOMP)Wtsi}/WtsiIeg
Alternative name	BEPD0009_1_E01
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Cir1^{tm3a(KOMP)Wtsi}
Gene/Transgene symbol	Cir1
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from KOMP ES clone BEPD0009_1_E01. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany

Disease and phenotype information

IMPC phenotypes (allele matching)

increased grip strength / IMPC
preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

increased grip strength / IMPC
preweaning lethality, complete penetrance / IMPC

MGI phenotypes (allele matching)

absent vertebral pedicles / MGI
rib bifurcation / MGI
rib fusion / MGI
scoliosis / MGI
overriding aortic valve / MGI
abnormal atrioventricular cushion morphology / MGI
abnormal large intestine morphology / MGI
abnormal ureter morphology / MGI
abnormal thyroid gland morphology / MGI
abnormal forebrain morphology / MGI
small kidney / MGI
decreased rib number / MGI
abnormal eye muscle morphology / MGI
abnormal Mullerian duct morphology / MGI
abnormal Wolffian duct morphology / MGI
abnormal optic cup morphology / MGI
occipital bone foramen / MGI
fusion of vertebral arches / MGI
decreased cervical vertebrae number / MGI
perimembraneous ventricular septal defect / MGI
abnormal pulmonary valve cusp morphology / MGI
abnormal hypoglossal nerve topology / MGI
subcutaneous edema / MGI
absent costovertebral joint / MGI
absent intracranial segment of vertebral artery / MGI
multiple persisting craniopharyngeal ducts / MGI
additional anastomosis between intracranial vertebral arteries / MGI
heterochrony / MGI

MGI phenotypes (gene matching)

absent vertebral pedicles / MGI
rib bifurcation / MGI
rib fusion / MGI
scoliosis / MGI
overriding aortic valve / MGI
abnormal atrioventricular cushion morphology / MGI
abnormal large intestine morphology / MGI
abnormal ureter morphology / MGI
abnormal thyroid gland morphology / MGI
abnormal forebrain morphology / MGI
small kidney / MGI
decreased rib number / MGI
abnormal eye muscle morphology / MGI
abnormal Mullerian duct morphology / MGI
abnormal Wolffian duct morphology / MGI
abnormal optic cup morphology / MGI
occipital bone foramen / MGI
fusion of vertebral arches / MGI
decreased cervical vertebrae number / MGI
perimembraneous ventricular septal defect / MGI
abnormal pulmonary valve cusp morphology / MGI
abnormal hypoglossal nerve topology / MGI
subcutaneous edema / MGI
absent costovertebral joint / MGI
absent intracranial segment of vertebral artery / MGI
multiple persisting craniopharyngeal ducts / MGI
additional anastomosis between intracranial vertebral arteries / MGI
heterochrony / MGI
persistent trigeminal artery / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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