C57BL/6N-A^tm1Brd Camsap3^{tm1a(EUCOMM)Wtsi}/WtsiPh

Status	Available to order
EMMA ID	EM:11496
International strain name	C57BL/6N-A^tm1Brd Camsap3^{tm1a(EUCOMM)Wtsi}/WtsiPh
Alternative name	EPD0453_4_A04
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Camsap3^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Camsap3
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0453_4_A04. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Institute of Molecular Genetics, Prague, Czech Republic

Disease and phenotype information

IMPC phenotypes (allele matching)

decreased bone mineral density / IMPC
decreased circulating HDL cholesterol level / IMPC
increased leukocyte cell number / IMPC
increased neutrophil cell number / IMPC
decreased body length / IMPC
abnormal lens morphology / IMPC
cataract / IMPC
fused cornea and lens / IMPC
abnormal cornea morphology / IMPC
corneal opacity / IMPC
trunk curl / IMPC
male infertility / IMPC
female infertility / IMPC
decreased hemoglobin content / IMPC
increased circulating alkaline phosphatase level / IMPC
increased circulating chloride level / IMPC
increased circulating glycerol level / IMPC
abnormal bone structure / IMPC
decreased lean body mass / IMPC
abnormal auditory brainstem response / IMPC
decreased circulating cholesterol level / IMPC
increased circulating thyroxine level / IMPC
decreased circulating glucose level / IMPC
decreased blood urea nitrogen level / IMPC
increased circulating sodium level / IMPC
absent pinna reflex / IMPC
increased gamma-delta T cell number / IMPC
increased total body fat amount / IMPC
decreased bone mineral content / IMPC
preweaning lethality, incomplete penetrance / IMPC

IMPC phenotypes (gene matching)

decreased circulating HDL cholesterol level / IMPC
increased leukocyte cell number / IMPC
corneal opacity / IMPC
female infertility / IMPC
increased circulating alkaline phosphatase level / IMPC
increased total body fat amount / IMPC
fused cornea and lens / IMPC
increased circulating thyroxine level / IMPC
cataract / IMPC
trunk curl / IMPC
decreased circulating cholesterol level / IMPC
abnormal cornea morphology / IMPC
increased gamma-delta T cell number / IMPC
decreased bone mineral density / IMPC
decreased circulating glucose level / IMPC
abnormal lens morphology / IMPC
decreased body length / IMPC
increased circulating glycerol level / IMPC
abnormal auditory brainstem response / IMPC
decreased lean body mass / IMPC
abnormal bone structure / IMPC
increased circulating sodium level / IMPC
decreased bone mineral content / IMPC
male infertility / IMPC
absent pinna reflex / IMPC
increased neutrophil cell number / IMPC
decreased hemoglobin content / IMPC
preweaning lethality, incomplete penetrance / IMPC
increased circulating chloride level / IMPC
decreased blood urea nitrogen level / IMPC

MGI phenotypes (allele matching)

abnormal forebrain morphology / MGI
abnormal dorsal root ganglion morphology / MGI
small lens / MGI
abnormal inferior vena cava morphology / MGI
abnormal cervical rib / MGI
abnormal liver parenchyma morphology / MGI
bicuspid aortic valve / MGI
abnormal ductus venosus valve morphology / MGI

MGI phenotypes (gene matching)

abnormal forebrain morphology / MGI
abnormal dorsal root ganglion morphology / MGI
decreased body weight / MGI
decreased body size / MGI
small lens / MGI
no abnormal phenotype detected / MGI
abnormal inferior vena cava morphology / MGI
abnormal small intestinal villus morphology / MGI
abnormal cervical rib / MGI
abnormal liver parenchyma morphology / MGI
bicuspid aortic valve / MGI
postnatal lethality, incomplete penetrance / MGI
abnormal ductus venosus valve morphology / MGI
retrolental blood / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

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C57BL/6N-Atm1Brd Camsap3tm1a(EUCOMM)Wtsi/WtsiPh