B6N.129(Cg)-Panx1^tm1.1Fama/Cnrm

Status	Available to order
EMMA ID	EM:11476
International strain name	B6N.129(Cg)-Panx1^tm1.1Fama/Cnrm
Alternative name	Panx1tm1Mony
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Panx1^tm1.1Fama
Gene/Transgene symbol	Panx1

Information from provider

Provider	Fabio Mammano
Provider affiliation	IBCN, CNR
Additional owner	Prof. Hannah Monyer, Abt. Klinische Neurobiologie des Universitätsklinikums, DKFZ, Heidelberg, Germany
Genetic information	These mice were generated by introducing a lacZ and a neomycin cassette within exon 1 of Panx1. After removal of the neomycin cassette by breeding with cre recombinase–deleter, mice were backcrossed to C57BL/6. Breeding of heterozygous mice yielded Panx1-/- mice at a Mendelian ratio.
Phenotypic information	Homozygous: The mice are viable and show no obvious developmental or behavioral abnormality. Heterozygous: The mice are viable and show no obvious developmental or behavioral abnormality.
Breeding history	Homozygous Panx1-/- mice were outcrossed to C57BL/6N after arrival in Monterotondo. More than 5 backcrosses to C57BL/6N were performed.
References	ATP release through connexin hemichannels and gap junction transfer of second messengers propagate Ca2+ signals across the inner ear.;Anselmi Fabio, Hernandez Victor H, Crispino Giulia, Seydel Anke, Ortolano Saida, Roper Stephen D, Kessaris Nicoletta, Richardson William, Rickheit Gesa, Filippov Mikhail A, Monyer Hannah, Mammano Fabio, ;2008;Proceedings of the National Academy of Sciences of the United States of America;105;18770-5; 19047635 Pannexins in ischemia-induced neurodegeneration.;Bargiotas Panagiotis, Krenz Antje, Hormuzdi Sheriar G, Ridder Dirk A, Herb Anne, Barakat Waleed, Penuela Silvia, von Engelhardt Jakob, Monyer Hannah, Schwaninger Markus, ;2011;Proceedings of the National Academy of Sciences of the United States of America;108;20772-7; 22147915 Promises and pitfalls of a Pannexin1 transgenic mouse line.;Hanstein Regina, Negoro Hiromitsu, Patel Naman K, Charollais Anne, Meda Paolo, Spray David C, Suadicani Sylvia O, Scemes Eliana, ;2013;Frontiers in pharmacology;4;61; 23675350 Mouse Panx1 Is Dispensable for Hearing Acquisition and Auditory Function.;Zorzi Veronica, Paciello Fabiola, Ziraldo Gaia, Peres Chiara, Mazzarda Flavia, Nardin Chiara, Pasquini Miriam, Chiani Francesco, Raspa Marcello, Scavizzi Ferdinando, Carrer Andrea, Crispino Giulia, Ciubotaru Catalin D, Monyer Hannah, Fetoni Anna R, M Salvatore Anna, Mammano Fabio, ;2017;Frontiers in molecular neuroscience;10;379; 29234270 Mouse Panx1 Is Dispensable for Hearing Acquisition and Auditory Function.;Zorzi Veronica, Paciello Fabiola, Ziraldo Gaia, Peres Chiara, Mazzarda Flavia, Nardin Chiara, Pasquini Miriam, Chiani Francesco, Raspa Marcello, Scavizzi Ferdinando, Carrer Andrea, Crispino Giulia, Ciubotaru Catalin D, Monyer Hannah, Fetoni Anna R, M Salvatore Anna, Mammano Fabio, ;2017;Frontiers in molecular neuroscience;10;379; 29234270 Organ-on-chip model shows that ATP release through connexin hemichannels drives spontaneous Ca²⁺ signaling in non-sensory cells of the greater epithelial ridge in the developing cochlea.;Mazzarda Flavia, D'Elia Annunziata, Massari Roberto, De Ninno Adele, Bertani Francesca Romana, Businaro Luca, Ziraldo Gaia, Zorzi Veronica, Nardin Chiara, Peres Chiara, Chiani Francesco, Tettey-Matey Abraham, Raspa Marcello, Scavizzi Ferdinando, Soluri Alessandro, Salvatore Anna Maria, Yang Jun, Mammano Fabio, ;2020;Lab on a chip;20;3011-3023; 32700707
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Female infertility due to oocyte meiotic arrest / Orphanet_488191

IMPC phenotypes (gene matching)

decreased mean corpuscular hemoglobin / IMPC
decreased thigmotaxis / IMPC
abnormal behavior / IMPC
decreased circulating serum albumin level / IMPC

MGI phenotypes (allele matching)

decreased susceptibility to pharmacologically induced seizures / MGI
abnormal neuron physiology / MGI
hearing/vestibular/ear phenotype / MGI

MGI phenotypes (gene matching)

abnormal respiratory system physiology / MGI
abnormal T cell physiology / MGI
decreased susceptibility to pharmacologically induced seizures / MGI
increased retinal ganglion cell number / MGI
decreased neuron apoptosis / MGI
abnormal neuron physiology / MGI
abnormal response to injury / MGI
hearing/vestibular/ear phenotype / MGI
immune system phenotype / MGI
abnormal cell physiology / MGI

Literature references

ATP release through connexin hemichannels and gap junction transfer of second messengers propagate Ca2+ signals across the inner ear.;Anselmi Fabio, Hernandez Victor H, Crispino Giulia, Seydel Anke, Ortolano Saida, Roper Stephen D, Kessaris Nicoletta, Richardson William, Rickheit Gesa, Filippov Mikhail A, Monyer Hannah, Mammano Fabio, ;2008;Proceedings of the National Academy of Sciences of the United States of America;105;18770-5; 19047635
Pannexins in ischemia-induced neurodegeneration.;Bargiotas Panagiotis, Krenz Antje, Hormuzdi Sheriar G, Ridder Dirk A, Herb Anne, Barakat Waleed, Penuela Silvia, von Engelhardt Jakob, Monyer Hannah, Schwaninger Markus, ;2011;Proceedings of the National Academy of Sciences of the United States of America;108;20772-7; 22147915
Promises and pitfalls of a Pannexin1 transgenic mouse line.;Hanstein Regina, Negoro Hiromitsu, Patel Naman K, Charollais Anne, Meda Paolo, Spray David C, Suadicani Sylvia O, Scemes Eliana, ;2013;Frontiers in pharmacology;4;61; 23675350
Mouse Panx1 Is Dispensable for Hearing Acquisition and Auditory Function.;Zorzi Veronica, Paciello Fabiola, Ziraldo Gaia, Peres Chiara, Mazzarda Flavia, Nardin Chiara, Pasquini Miriam, Chiani Francesco, Raspa Marcello, Scavizzi Ferdinando, Carrer Andrea, Crispino Giulia, Ciubotaru Catalin D, Monyer Hannah, Fetoni Anna R, M Salvatore Anna, Mammano Fabio, ;2017;Frontiers in molecular neuroscience;10;379; 29234270
Organ-on-chip model shows that ATP release through connexin hemichannels drives spontaneous Ca²⁺ signaling in non-sensory cells of the greater epithelial ridge in the developing cochlea.;Mazzarda Flavia, D'Elia Annunziata, Massari Roberto, De Ninno Adele, Bertani Francesca Romana, Businaro Luca, Ziraldo Gaia, Zorzi Veronica, Nardin Chiara, Peres Chiara, Chiani Francesco, Tettey-Matey Abraham, Raspa Marcello, Scavizzi Ferdinando, Soluri Alessandro, Salvatore Anna Maria, Yang Jun, Mammano Fabio, ;2020;Lab on a chip;20;3011-3023; 32700707

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.

B6N.129(Cg)-Panx1tm1.1Fama/Cnrm