IMPC phenotypes (gene matching)
C57BL/6J-Ythdc2em2Embrp/Cnrm
| Status | Available to order |
| EMMA ID | EM:11456 |
| Citation information | RRID:IMSR_EM:11456 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6J-Ythdc2em2Embrp/Cnrm |
| Alternative name | Ythdc2 KO (C57BL6J) |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Ythdc2em2Embrp |
| Gene/Transgene symbol | Ythdc2 |
Information from provider
| Provider | Ramesh PILLAI |
| Provider affiliation | Department of Molecular Biology, University of Geneva |
| Genetic information | Deletion of the exon7 of the Ythdc2 gene to generate a knock-out mouse. Exon7 encodes for the catalytic residues required for ATPase activity of the protein. |
| Phenotypic information | Homozygous:Homozygous males and females are infertile.Heterozygous:Heterozygous males and females display no detectable phenotypes. They are viable and fertile. |
| Breeding history | Founders generated using CRISPR-Cas9 injection into 1-cell embryos of the C57BL/6J genetic background were crossed with wild-type C57BL/6J partners to produce heterozygous animals. These heterozygous animals were then intercrossed over 5 generations to generate the current animals being sent to EMMA. |
| References |
|
| Homozygous fertile | no |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
MGI phenotypes (allele matching)
- abnormal ovarian follicle morphology / MGI
- male infertility / MGI
- female infertility / MGI
- absent ovarian follicles / MGI
- testicular atrophy / MGI
- ovary atrophy / MGI
- azoospermia / MGI
- abnormal female meiosis / MGI
- decreased oocyte number / MGI
- arrest of male meiosis / MGI
- increased male germ cell apoptosis / MGI
MGI phenotypes (gene matching)
- small ovary / MGI
- abnormal ovarian follicle morphology / MGI
- small testis / MGI
- small seminiferous tubules / MGI
- male infertility / MGI
- female infertility / MGI
- decreased germ cell number / MGI
- absent ovarian follicles / MGI
- testicular atrophy / MGI
- ovary atrophy / MGI
- decreased testis weight / MGI
- azoospermia / MGI
- abnormal female meiosis / MGI
- decreased oocyte number / MGI
- arrest of male meiosis / MGI
- increased male germ cell apoptosis / MGI
Literature references
- Regulation of m6A Transcripts by the 3'→5' RNA Helicase YTHDC2 Is Essential for a Successful Meiotic Program in the Mammalian Germline.;Wojtas Magdalena Natalia, Pandey Radha Raman, Mendel Mateusz, Homolka David, Sachidanandam Ravi, Pillai Ramesh S, ;2017;Molecular cell;68;374-387.e12; 29033321