C57BL/6J-Ythdc2em2Embrp/Cnrm
Status | Available to order |
EMMA ID | EM:11456 |
International strain name | C57BL/6J-Ythdc2em2Embrp/Cnrm |
Alternative name | Ythdc2 KO (C57BL6J) |
Strain type | Endonuclease-mediated |
Allele/Transgene symbol | Ythdc2em2Embrp |
Gene/Transgene symbol | Ythdc2 |
Information from provider
Provider | Ramesh PILLAI |
Provider affiliation | Department of Molecular Biology, University of Geneva |
Genetic information | Deletion of the exon7 of the Ythdc2 gene to generate a knock-out mouse. Exon7 encodes for the catalytic residues required for ATPase activity of the protein. |
Phenotypic information | Homozygous:Homozygous males and females are infertile.Heterozygous:Heterozygous males and females display no detectable phenotypes. They are viable and fertile. |
Breeding history | Founders generated using CRISPR-Cas9 injection into 1-cell embryos of the C57BL/6J genetic background were crossed with wild-type C57BL/6J partners to produce heterozygous animals. These heterozygous animals were then intercrossed over 5 generations to generate the current animals being sent to EMMA. |
References |
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Homozygous fertile | no |
Homozygous viable | yes |
Homozygous matings required | no |
Immunocompromised | no |
Information from EMMA
Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
IMPC phenotypes (gene matching)
MGI phenotypes (allele matching)
- abnormal ovarian follicle morphology / MGI
- male infertility / MGI
- female infertility / MGI
- absent ovarian follicles / MGI
- testicular atrophy / MGI
- ovary atrophy / MGI
- azoospermia / MGI
- abnormal female meiosis / MGI
- decreased oocyte number / MGI
- arrest of male meiosis / MGI
- increased male germ cell apoptosis / MGI
MGI phenotypes (gene matching)
- small ovary / MGI
- abnormal ovarian follicle morphology / MGI
- small testis / MGI
- small seminiferous tubules / MGI
- male infertility / MGI
- female infertility / MGI
- decreased germ cell number / MGI
- absent ovarian follicles / MGI
- testicular atrophy / MGI
- ovary atrophy / MGI
- decreased testis weight / MGI
- azoospermia / MGI
- abnormal female meiosis / MGI
- decreased oocyte number / MGI
- arrest of male meiosis / MGI
- increased male germ cell apoptosis / MGI
Literature references
- Regulation of m6A Transcripts by the 3'→5' RNA Helicase YTHDC2 Is Essential for a Successful Meiotic Program in the Mammalian Germline.;Wojtas Magdalena Natalia, Pandey Radha Raman, Mendel Mateusz, Homolka David, Sachidanandam Ravi, Pillai Ramesh S, ;2017;Molecular cell;68;374-387.e12; 29033321
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