B6;D2-Tg(Cd4-cre)1Cwi/CwiCnrm

Status	Available to order
EMMA ID	EM:01139
International strain name	B6;D2-Tg(Cd4-cre)1Cwi/CwiCnrm
Alternative name	CD4-Cre
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(Cd4-cre)1Cwi
Gene/Transgene symbol	Tg(Cd4-cre)1Cwi

Information from provider

Provider	Chris Wilson
Provider affiliation	Department of Immunology, University of Washington, Dept. Immunology
Genetic information	CD4-cre mice specifically express the P1 bacteriophage cre recombinase in T-cells.
Phenotypic information	CD4-cre mice have been shown to efficiently delete loxP-flanked genes in the T-cell lineage.
Breeding history	(C57BL/6 x DBA/2)F2 background; backcrossed to C57BL/6 (2-3 generations).
References	A critical role for Dnmt1 and DNA methylation in T cell development, function, and survival.;Lee P P, Fitzpatrick D R, Beard C, Jessup H K, Lehar S, Makar K W, Pérez-Melgosa M, Sweetser M T, Schlissel M S, Nguyen S, Cherry S R, Tsai J H, Tucker S M, Weaver W M, Kelso A, Jaenisch R, Wilson C B, ;2001;Immunity;15;763-74; 11728338

Information from EMMA

Archiving centre	CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Literature references

A critical role for Dnmt1 and DNA methylation in T cell development, function, and survival.;Lee P P, Fitzpatrick D R, Beard C, Jessup H K, Lehar S, Makar K W, Pérez-Melgosa M, Sweetser M T, Schlissel M S, Nguyen S, Cherry S R, Tsai J H, Tucker S M, Weaver W M, Kelso A, Jaenisch R, Wilson C B, ;2001;Immunity;15;763-74; 11728338
MYC deficiency impairs the development of effector/memory T lymphocytes.;Nozais Mathis, Loosveld Marie, Pankaew Saran, Grosjean Clémence, Gentil Noémie, Quessada Julie, Nadel Bertrand, Mionnet Cyrille, Potier Delphine, Payet-Bornet Dominique, ;2021;iScience;24;102761; 34258568
Isolation and enrichment of mouse splenic T cells for ex vivo and in vivo T cell receptor stimulation assays.;Grosjean Clémence, Quessada Julie, Nozais Mathis, Loosveld Marie, Payet-Bornet Dominique, Mionnet Cyrille, ;2021;STAR protocols;2;100961; 34825221
Lack of NFATc1 SUMOylation prevents autoimmunity and alloreactivity.;Xiao Yin, Qureischi Musga, Dietz Lena, Vaeth Martin, Vallabhapurapu Subrahmanya D, Klein-Hessling Stefan, Klein Matthias, Liang Chunguang, König Anika, Serfling Edgar, Mottok Anja, Bopp Tobias, Rosenwald Andreas, Buttmann Mathias, Berberich Ingolf, Beilhack Andreas, Berberich-Siebelt Friederike, ;2021;The Journal of experimental medicine;218;101041; 32986812
PI3-Kinase p110α Deficiency Modulates T Cell Homeostasis and Function and Attenuates Experimental Allergic Encephalitis in Mature Mice.;Rojo José M, Montes-Casado María, Aragoneses-Fenoll Laura, Ojeda Gloria, Dianzani Umberto, Portolés Pilar, ;2021;International journal of molecular sciences;22;16258-63; 34445401
The PI-3-Kinase P110α Catalytic Subunit of T Lymphocytes Modulates Collagen-Induced Arthritis.;Montes-Casado María, Ojeda Gloria, Criado Gabriel, Rojo José M, Portolés Pilar, ;2021;International journal of molecular sciences;22;e102066; 34203838
Multiplexed single-cell RNA-sequencing of mouse thymic and splenic samples.;Pankaew Saran, Grosjean Clémence, Quessada Julie, Loosveld Marie, Potier Delphine, Payet-Bornet Dominique, Nozais Mathis, ;2021;STAR protocols;3;2079-83; 36475567
Dependence on nuclear factor of activated T-cells (NFAT) levels discriminates conventional T cells from Foxp3+ regulatory T cells.;Vaeth Martin, Schliesser Ulrike, Müller Gerd, Reissig Sonja, Satoh Kazuki, Tuettenberg Andrea, Jonuleit Helmut, Waisman Ari, Müller Martin R, Serfling Edgar, Sawitzki Birgit S, Berberich-Siebelt Friederike, ;2012;Proceedings of the National Academy of Sciences of the United States of America;109;332; 22991461
T-cell autophagy deficiency increases mortality and suppresses immune responses after sepsis.;Lin Chih-Wen, Lo Steven, Hsu Chin, Hsieh Chi-Hsun, Chang Ya-Fang, Hou Bao-Sheng, Kao Ying-Hsien, Lin Chih-Che, Yu Ming-Lung, Yuan Shyng-Shiou, Hsieh Ya-Ching, ;2014;PloS one;9;; 25029098
Cutting edge: suppression of GM-CSF expression in murine and human T cells by IL-27.;Young Andrew, Linehan Eimear, Hams Emily, O'Hara Hall Aisling C, McClurg Angela, Johnston James A, Hunter Christopher A, Fallon Padraic G, Fitzgerald Denise C, ;2012;Journal of immunology (Baltimore, Md. : 1950);189;; 22837488
T-Cell-Specific Loss of the PI-3-Kinase p110α Catalytic Subunit Results in Enhanced Cytokine Production and Antitumor Response.;Aragoneses-Fenoll Laura, Ojeda Gloria, Montes-Casado María, Acosta-Ampudia Yeny, Dianzani Umberto, Portolés Pilar, Rojo José M, ;2018;Frontiers in immunology;9;; 29535720

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Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

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Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

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