B6.Cg-Tg(KRT14-cre)1Cgn/CgnCnrm
| Status | Available to order |
| EMMA ID | EM:01131 |
| International strain name | B6.Cg-Tg(KRT14-cre)1Cgn/CgnCnrm |
| Alternative name | K14-Cre |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(KRT14-cre)1Cgn |
| Gene/Transgene symbol | Tg(KRT14-cre)1Cgn |
Information from provider
| Provider | Thomas Krieg |
| Provider affiliation | Department of Dermatology, University of Cologne, Dept. Dermatology |
| Genetic information | The expression vector pBBEB-K14 contains 2.18 kb of the human keratin 14 promoter (Staggers et al., 1995) and the SV40 polyA signal of the plasmid K14(c)-luc (J. Kudlow, Univ. of Alabama at Birmingham, USA). The nuclear localisation signal (NLS) containing the cre recombinase open reading frame was excised from the vector pGK-cre (K. Rajewsky and W. Mueller, Univ. of Cologne, Germany) and ligated into the vector pBBEBK14 between the K14 promoter and the SV40 polyA signal. For the generation of transgenic mice the K14 promoter-cre-SV40polyA expression cassette was excised and microinjected in fertilized B6xB6D2F2 oocytes. |
| Phenotypic information | If inherited from the father, the K14-cre#47 transgene confers tissue specific deletion of loxP-flanked DNA segments in the epidermis, tongue epithelium and thymic epithelium. No deletion is observed in the esophagus or in the stomach. If the K14cre#47 transgene is inherited from the mother, a dominant maternal phenotype is observed: deletion of loxP-flanked DNA segments occurs in all of the offsprings tissues, even in those littermates that did not inherit the transgene from the mother. This indicates that the transgene is expressed in the oocytes and that the protein is still active when the transgene is lost during meiosis. The stability of the protein is sufficient to confer deletion of paternally inherited loxP-flanked DNA segments after fertilization. |
| Breeding history | Generated in C57BL/6 x (C57BL/6 x DBA/2)F2 oocytes; backcrossed to C57BL/6 (4 generations) |
| References |
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Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Literature references
- Keratin 14 Cre transgenic mice authenticate keratin 14 as an oocyte-expressed protein.;Hafner Martin, Wenk Jutta, Nenci Arianna, Pasparakis Manolis, Scharffetter-Kochanek Karin, Smyth Neil, Peters Thorsten, Kess Daniel, Holtkötter Olaf, Shephard Pierre, Kudlow Jeffrey E, Smola Hans, Haase Ingo, Schippers Angela, Krieg Thomas, Müller Werner, ;2004;Genesis (New York, N.Y. : 2000);38;176-81; 15083518