C57BL/6N-Crb2^{tm1c(KOMP)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:11129
International strain name	C57BL/6N-Crb2^{tm1c(KOMP)Wtsi}/WtsiH
Alternative name	EPD0603_1_B06
Strain type	Targeted Mutant Strains : Targeted Conditional
Allele/Transgene symbol	Crb2^{tm1c(KOMP)Wtsi}
Gene/Transgene symbol	Crb2
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This line originates from KOMP ES clone EPD0603_1_B06, after breeding with a Flp recombinase deleter line to convert the original targeted allele tm1a (knock-out first allele) into a conditional allele tm1c. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	heterozygous C57BL/6NTacUSA
Breeding at archiving centre	C57BL/6NTac background

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Genetic steroid-resistant nephrotic syndrome / Orphanet_656
Ventriculomegaly-cystic kidney disease / Orphanet_443988

IMPC phenotypes (gene matching)

preweaning lethality, complete penetrance / IMPC
abnormal retina morphology / IMPC
abnormal vibrissa morphology / IMPC

MGI phenotypes (gene matching)

abnormal foregut morphology / MGI
open neural tube / MGI
abnormal mesoderm development / MGI
abnormal endoderm development / MGI
abnormal somite development / MGI
abnormal gastrulation / MGI
increased embryo size / MGI
abnormal embryonic tissue morphology / MGI
no abnormal phenotype detected / MGI
abnormal primitive streak morphology / MGI
abnormal chorioallantoic fusion / MGI
abnormal notochord morphology / MGI
abnormal digestive system development / MGI
abnormal vitelline vasculature morphology / MGI
abnormal embryonic epiblast morphology / MGI
failure of initiation of embryo turning / MGI
abnormal embryonic neuroepithelium morphology / MGI
small allantois / MGI
failure of chorioallantoic fusion / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
abnormal visceral yolk sac blood island morphology / MGI
abnormal head fold morphology / MGI
absent heart tube / MGI
small amniotic cavity / MGI
abnormal anterior primitive streak morphology / MGI

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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