B6.Cg-Zfp36tm1Pjb/Flmg

Status

Available to order

EMMA IDEM:11082
International strain nameB6.Cg-Zfp36tm1Pjb/Flmg
Alternative nameB6.Cg-Zfp36/Flmg
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolZfp36tm1Pjb
Gene/Transgene symbolZfp36

Information from provider

ProviderPerry Blackshear
Provider affiliationRall Building , National Institute of Environmental Health Sciences (NIEHS)
Genetic informationExon 2 was disrupted by the insertion of a neomycin selection cassette. Multiple stop codons were generated upstream of sequence encoding two putative zinc fingers. Northern blot analysis showed an absence of normal transcript and identified an aberrant transcript containing neo. Neither normal nor truncated protein was detected by Western blot analysis of mouse embryonic fibroblasts derived from homozygous mutant mice.
Phenotypic informationHomozygous:
Tristetraprolin (Ttp, Zfp36) knock-out mice develop a complex and severe inflammatory syndrome that involves arthritis, autoimmunity, cachexia, dermatitis, and myeloid hyperplasia.

Heterozygous:
TTP heterozygous mice are normal and fertile.
References
  • A pathogenetic role for TNF alpha in the syndrome of cachexia, arthritis, and autoimmunity resulting from tristetraprolin (TTP) deficiency.;Taylor G A, Carballo E, Lee D M, Lai W S, Thompson M J, Patel D D, Schenkman D I, Gilkeson G S, Broxmeyer H E, Haynes B F, Blackshear P J, ;1996;Immunity;4;445-54; 8630730
  • Endothelial dysfunction in tristetraprolin-deficient mice is not caused by enhanced tumor necrosis factor-α expression.;Bollmann Franziska, Wu Zhixiong, Oelze Matthias, Siuda Daniel, Xia Ning, Henke Jenny, Daiber Andreas, Li Huige, Stumpo Deborah J, Blackshear Perry J, Kleinert Hartmut, Pautz Andrea, ;2014;The Journal of biological chemistry;289;15653-65; 24727475
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreB.S.R.C. Alexander Fleming, Vari, Greece
Animals used for archivingheterozygous C57BL/6

Disease and phenotype information

MGI allele-associated human disease models

IMPC phenotypes (gene matching)
  • decreased mean corpuscular volume / IMPC
  • increased leukocyte cell number / IMPC
  • increased fasting circulating glucose level / IMPC
MGI phenotypes (allele matching)
  • increased neutrophil cell number / MGI
  • increased monocyte cell number / MGI
  • increased granulocyte number / MGI
  • abnormal vasodilation / MGI
  • vascular inflammation / MGI
  • abnormal macrophage physiology / MGI
  • decreased systemic arterial blood pressure / MGI
  • arthritis / MGI
  • oxidative stress / MGI
  • abnormal nitric oxide homeostasis / MGI
  • decreased lymphocyte cell number / MGI
  • homeostasis/metabolism phenotype / MGI
  • thrombocytosis / MGI
  • increased cellular sensitivity to oxidative stress / MGI
  • increased leukocyte cell number / MGI
  • extramedullary hematopoiesis / MGI
  • alopecia / MGI
  • enlarged spleen / MGI
  • spleen hyperplasia / MGI
  • enlarged lymph nodes / MGI
  • dermatitis / MGI
  • decreased body weight / MGI
  • postnatal growth retardation / MGI
  • thymus hypoplasia / MGI
  • conjunctivitis / MGI
  • heart inflammation / MGI
  • liver inflammation / MGI
  • premature death / MGI
  • abnormal thymus cortex morphology / MGI
  • abnormal thymus medulla morphology / MGI
  • abnormal renal glomerulus morphology / MGI
  • absent subcutaneous adipose tissue / MGI
MGI phenotypes (gene matching)
  • increased leukocyte cell number / MGI
  • increased neutrophil cell number / MGI
  • increased monocyte cell number / MGI
  • extramedullary hematopoiesis / MGI
  • increased granulocyte number / MGI
  • alopecia / MGI
  • enlarged spleen / MGI
  • spleen hyperplasia / MGI
  • enlarged lymph nodes / MGI
  • dermatitis / MGI
  • decreased body weight / MGI
  • abnormal vasodilation / MGI
  • postnatal growth retardation / MGI
  • thymus hypoplasia / MGI
  • conjunctivitis / MGI
  • heart inflammation / MGI
  • liver inflammation / MGI
  • vascular inflammation / MGI
  • decreased inflammatory response / MGI
  • premature death / MGI
  • abnormal thymus cortex morphology / MGI
  • abnormal thymus medulla morphology / MGI
  • abnormal macrophage physiology / MGI
  • decreased systemic arterial blood pressure / MGI
  • arthritis / MGI
  • decreased susceptibility to induced arthritis / MGI
  • oxidative stress / MGI
  • abnormal nitric oxide homeostasis / MGI
  • decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
  • decreased lymphocyte cell number / MGI
  • abnormal renal glomerulus morphology / MGI
  • homeostasis/metabolism phenotype / MGI
  • thrombocytosis / MGI
  • increased cellular sensitivity to oxidative stress / MGI
  • absent subcutaneous adipose tissue / MGI

Literature references

  • A pathogenetic role for TNF alpha in the syndrome of cachexia, arthritis, and autoimmunity resulting from tristetraprolin (TTP) deficiency.;Taylor G A, Carballo E, Lee D M, Lai W S, Thompson M J, Patel D D, Schenkman D I, Gilkeson G S, Broxmeyer H E, Haynes B F, Blackshear P J, ;1996;Immunity;4;445-54; 8630730
  • Endothelial dysfunction in tristetraprolin-deficient mice is not caused by enhanced tumor necrosis factor-α expression.;Bollmann Franziska, Wu Zhixiong, Oelze Matthias, Siuda Daniel, Xia Ning, Henke Jenny, Daiber Andreas, Li Huige, Stumpo Deborah J, Blackshear Perry J, Kleinert Hartmut, Pautz Andrea, ;2014;The Journal of biological chemistry;289;15653-65; 24727475

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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