B6.129S-Tnfrsf1a^tm1Gkl/Flmg

Status	Available to order
EMMA ID	EM:11071
International strain name	B6.129S-Tnfrsf1a^tm1Gkl/Flmg
Alternative name	B6.Cg-Tnfrsf1a/Flmg
Strain type	Targeted Mutant Strains : Conditional mutation
Allele/Transgene symbol	Tnfrsf1a^tm1Gkl
Gene/Transgene symbol	Tnfrsf1a

Information from provider

Provider	George Kollias
Provider affiliation	BSRC Fleming
Genetic information	A mutation deleting 15 nucleotides encoding amino acids 202-206 was introduced in exon 6 of mp55TNFR (Tnfrsf1a) gene. The targeting vector contained a 4.6-kb XbaI/SmaI genomic fragment as the left homology arm and a 4-kb Sma/HindIII genomic fragment, containing the mutation, as the right homology arm. A loxP flanked neo selection cassette (L2neo) was introduced into a SmaI site in intron 5 of Tnfrsf1a gene between the two homology arms and a PGK-tk selection marker was introduced at the 3' of targeting vector. Upon cre-mediated recombination, a cell-specific Tnfrsf1a-tm1.1Gkl allele is generated.
Phenotypic information	Homozygous: No overt phenotype. Homozygous mice are fertile but they are smaller than their littermates and in poor health so we do not mate the homozygous mice. Heterozygous: No overt phenotype
Breeding history	Homozygous mice and wild-type controls are generated by crossing heterozygous mice.
References	Tumor necrosis factor (TNF) receptor shedding controls thresholds of innate immune activation that balance opposing TNF functions in infectious and inflammatory diseases.;Xanthoulea Sofia, Pasparakis Manolis, Kousteni Stavroula, Brakebusch Cord, Wallach David, Bauer Jan, Lassmann Hans, Kollias George, ;2004;The Journal of experimental medicine;200;367-76; 15289505
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	B.S.R.C. Alexander Fleming, Vari, Greece
Animals used for archiving	homozygous C57BL/6, wild-type C57BL/6
Stage of embryos	2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Tumor necrosis factor receptor 1 associated periodic syndrome / Orphanet_32960

IMPC phenotypes (gene matching)

abnormal skin morphology / IMPC
increased large unstained cell number / IMPC
increased grip strength / IMPC
increased basophil cell number / IMPC
increased neutrophil cell number / IMPC
increased eosinophil cell number / IMPC
increased NK cell number / IMPC
abnormal cholesterol homeostasis / IMPC
decreased circulating calcium level / IMPC
increased lymphocyte cell number / IMPC
increased effector memory CD8-positive, alpha-beta T cell number / IMPC
increased circulating aspartate transaminase level / IMPC
enlarged heart / IMPC
increased leukocyte cell number / IMPC
decreased monocyte cell number / IMPC
abnormal heart morphology / IMPC
decreased neutrophil cell number / IMPC

MGI phenotypes (allele matching)

liver inflammation / MGI
increased sensitivity to induced morbidity/mortality / MGI
decreased susceptibility to bacterial infection / MGI
abnormal cytokine secretion / MGI
increased susceptibility to experimental autoimmune encephalomyelitis / MGI

MGI phenotypes (gene matching)

increased bone mineral density / MGI
abnormal Peyer's patch morphology / MGI
abnormal sleep pattern / MGI
abnormal immune system physiology / MGI
decreased IgG level / MGI
liver inflammation / MGI
lung inflammation / MGI
decreased inflammatory response / MGI
increased skin papilloma incidence / MGI
decreased tumor incidence / MGI
abnormal respiratory mechanics / MGI
abnormal airway responsiveness / MGI
abnormal lymph node B cell domain morphology / MGI
abnormal spleen germinal center morphology / MGI
abnormal Peyer's patch follicle morphology / MGI
decreased susceptibility to bacterial infection / MGI
increased susceptibility to bacterial infection / MGI
abnormal macrophage physiology / MGI
granulomatous inflammation / MGI
impaired skin barrier function / MGI
abnormal glutamate-mediated receptor currents / MGI
decreased circulating alanine transaminase level / MGI
abnormal cytokine secretion / MGI
no phenotypic analysis / MGI
abnormal nervous system physiology / MGI
abnormal response/metabolism to endogenous compounds / MGI
increased susceptibility to experimental autoimmune encephalomyelitis / MGI
decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
decreased susceptibility to autoimmune diabetes / MGI
increased susceptibility to parasitic infection / MGI
increased susceptibility to type I hypersensitivity reaction / MGI
decreased Peyer's patch number / MGI
abnormal follicular dendritic cell morphology / MGI
absent follicular dendritic cells / MGI
decreased IgG1 level / MGI
increased circulating tumor necrosis factor level / MGI
decreased circulating interleukin-6 level / MGI
decreased circulating interleukin-1 beta level / MGI
increased interleukin-6 secretion / MGI
decreased interleukin-6 secretion / MGI
abnormal chemokine secretion / MGI
decreased susceptibility to endotoxin shock / MGI
increased susceptibility to endotoxin shock / MGI
decreased physiological sensitivity to xenobiotic / MGI
increased sensitivity to induced cell death / MGI
increased sensitivity to induced morbidity/mortality / MGI
decreased sensitivity to induced morbidity/mortality / MGI
decreased sensitivity to xenobiotic induced morbidity/mortality / MGI
increased susceptibility to bacterial infection induced morbidity/mortality / MGI
abnormal neuron proliferation / MGI
abnormal oval cell physiology / MGI
tumor regression / MGI
impaired adaptive thermogenesis / MGI
decreased susceptibility to dopaminergic neuron neurotoxicity / MGI
decreased microglial cell number / MGI
impaired humoral immune response / MGI
impaired central nervous system regeneration / MGI

Literature references

Tumor necrosis factor (TNF) receptor shedding controls thresholds of innate immune activation that balance opposing TNF functions in infectious and inflammatory diseases.;Xanthoulea Sofia, Pasparakis Manolis, Kousteni Stavroula, Brakebusch Cord, Wallach David, Bauer Jan, Lassmann Hans, Kollias George, ;2004;The Journal of experimental medicine;200;367-76; 15289505

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6.129S-Tnfrsf1atm1Gkl/Flmg