C3HeB/FeJ-Scube3^m1Mhda/Ieg

Status	Available to order
EMMA ID	EM:10872
International strain name	C3HeB/FeJ-Scube3^m1Mhda/Ieg
Alternative name	Scube3-KTA041 = Scube3N294K/N294K
Strain type	Induced Mutant Strains : Chemically-induced
Allele/Transgene symbol	Scube3^m1Mhda
Gene/Transgene symbol	Scube3

Information from provider

Provider	Martin Hrabe de Angelis
Provider affiliation	Institute of Experimental Genetics, Helmholtz Zentrum München
Genetic information	A C to A transversion at nucleotide position 882 in exon 8 of Scube3 leads to an asparagine to lysine exchange at protein position 294 (N294K).
Phenotypic information	Homozygous: Morphological abnormalities of the skeleton, alterations of parameters relevant for bone metabolism, changes in renal function and hearing impairments. Heterozygous: No clear phenotype.
References	The First Scube3 Mutant Mouse Line with Pleiotropic Phenotypic Alterations.;Fuchs Helmut, Sabrautzki Sibylle, Przemeck Gerhard K H, Leuchtenberger Stefanie, Lorenz-Depiereux Bettina, Becker Lore, Rathkolb Birgit, Horsch Marion, Garrett Lillian, Östereicher Manuela A, Hans Wolfgang, Abe Koichiro, Sagawa Nobuho, Rozman Jan, Vargas-Panesso Ingrid L, Sandholzer Michael, Lisse Thomas S, Adler Thure, Aguilar-Pimentel Juan Antonio, Calzada-Wack Julia, Ehrhard Nicole, Elvert Ralf, Gau Christine, Hölter Sabine M, Micklich Katja, Moreth Kristin, Prehn Cornelia, Puk Oliver, Racz Ildiko, Stoeger Claudia, Vernaleken Alexandra, Michel Dian, Diener Susanne, Wieland Thomas, Adamski Jerzy, Bekeredjian Raffi, Busch Dirk H, Favor John, Graw Jochen, Klingenspor Martin, Lengger Christoph, Maier Holger, Neff Frauke, Ollert Markus, Stoeger Tobias, Yildirim Ali Önder, Strom Tim M, Zimmer Andreas, Wolf Eckhard, Wurst Wolfgang, Klopstock Thomas, Beckers Johannes, Gailus-Durner Valerie, Hrabé de Angelis Martin, ;2016;G3 (Bethesda, Md.);6;4035-4046; 27815347
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany

Disease and phenotype information

MGI phenotypes (allele matching)

abnormal inner ear morphology / MGI
abnormal malleus morphology / MGI
abnormal middle ear morphology / MGI
decreased bone mineral density / MGI
rib fusion / MGI
increased circulating calcium level / MGI
decreased circulating phosphate level / MGI
thin ventricular wall / MGI
abnormal interventricular septum morphology / MGI
kinked tail / MGI
short tail / MGI
decreased body size / MGI
abnormal anxiety-related response / MGI
hypoactivity / MGI
abnormal posture / MGI
polyuria / MGI
abnormal digit morphology / MGI
decreased circulating triglyceride level / MGI
decreased circulating free fatty acid level / MGI
decreased vertical activity / MGI
decreased urine protein level / MGI
abnormal thoracic vertebrae morphology / MGI
abnormal lumbar vertebrae morphology / MGI
abnormal metatarsal bone morphology / MGI
abnormal metacarpal bone morphology / MGI
short femur / MGI
decreased circulating glycerol level / MGI
decreased urine glucose level / MGI
abnormal heart left ventricle morphology / MGI
decreased lean body mass / MGI
abnormal pectoral girdle bone morphology / MGI
abnormal vertebral pedicle morphology / MGI
conductive hearing loss / MGI
increased bone resorption / MGI
abnormal tarsal bone morphology / MGI
abnormal middle ear ossicle morphology / MGI
decreased circulating cholesterol level / MGI
abnormal temporal bone morphology / MGI
improved glucose tolerance / MGI
decreased circulating serum albumin level / MGI
abnormal food intake / MGI
abnormal body composition / MGI
decreased urine albumin level / MGI
increased circulating creatinine level / MGI
decreased circulating glucose level / MGI
increased blood urea nitrogen level / MGI
decreased circulating total protein level / MGI
decreased pulmonary respiratory rate / MGI
abnormal enzyme/coenzyme activity / MGI
increased circulating potassium level / MGI
abnormal bone ossification / MGI
fused phalanges / MGI
increased blood uric acid level / MGI
decreased grip strength / MGI
decreased bone mineral content / MGI
abnormal malleus head morphology / MGI
prolonged QRS complex duration / MGI
decreased urine uric acid level / MGI
decreased urine creatinine level / MGI
increased alkaline phosphatase activity / MGI
increased fluid intake / MGI
increased or absent threshold for auditory brainstem response / MGI
abnormal renal filtration / MGI
decreased body fat mass / MGI
small incus / MGI
small middle ear ossicles / MGI

MGI phenotypes (gene matching)

abnormal inner ear morphology / MGI
abnormal malleus morphology / MGI
abnormal middle ear morphology / MGI
decreased bone mineral density / MGI
rib fusion / MGI
increased circulating calcium level / MGI
decreased circulating phosphate level / MGI
thin ventricular wall / MGI
abnormal interventricular septum morphology / MGI
kinked tail / MGI
short tail / MGI
decreased body size / MGI
abnormal anxiety-related response / MGI
hypoactivity / MGI
abnormal posture / MGI
polyuria / MGI
abnormal digit morphology / MGI
no abnormal phenotype detected / MGI
decreased circulating triglyceride level / MGI
decreased circulating free fatty acid level / MGI
decreased vertical activity / MGI
decreased urine protein level / MGI
abnormal thoracic vertebrae morphology / MGI
abnormal lumbar vertebrae morphology / MGI
abnormal metatarsal bone morphology / MGI
abnormal metacarpal bone morphology / MGI
short femur / MGI
decreased circulating glycerol level / MGI
decreased urine glucose level / MGI
abnormal heart left ventricle morphology / MGI
decreased lean body mass / MGI
abnormal pectoral girdle bone morphology / MGI
abnormal vertebral pedicle morphology / MGI
conductive hearing loss / MGI
increased bone resorption / MGI
abnormal tarsal bone morphology / MGI
abnormal middle ear ossicle morphology / MGI
decreased circulating cholesterol level / MGI
abnormal temporal bone morphology / MGI
improved glucose tolerance / MGI
decreased circulating serum albumin level / MGI
abnormal food intake / MGI
abnormal body composition / MGI
decreased urine albumin level / MGI
increased circulating creatinine level / MGI
decreased circulating glucose level / MGI
increased blood urea nitrogen level / MGI
decreased circulating total protein level / MGI
decreased pulmonary respiratory rate / MGI
abnormal enzyme/coenzyme activity / MGI
increased circulating potassium level / MGI
abnormal bone ossification / MGI
fused phalanges / MGI
increased blood uric acid level / MGI
decreased grip strength / MGI
decreased bone mineral content / MGI
abnormal malleus head morphology / MGI
prolonged QRS complex duration / MGI
decreased urine uric acid level / MGI
decreased urine creatinine level / MGI
increased alkaline phosphatase activity / MGI
increased fluid intake / MGI
increased or absent threshold for auditory brainstem response / MGI
abnormal renal filtration / MGI
decreased body fat mass / MGI
small incus / MGI
small middle ear ossicles / MGI

Literature references

The First Scube3 Mutant Mouse Line with Pleiotropic Phenotypic Alterations.;Fuchs Helmut, Sabrautzki Sibylle, Przemeck Gerhard K H, Leuchtenberger Stefanie, Lorenz-Depiereux Bettina, Becker Lore, Rathkolb Birgit, Horsch Marion, Garrett Lillian, Östereicher Manuela A, Hans Wolfgang, Abe Koichiro, Sagawa Nobuho, Rozman Jan, Vargas-Panesso Ingrid L, Sandholzer Michael, Lisse Thomas S, Adler Thure, Aguilar-Pimentel Juan Antonio, Calzada-Wack Julia, Ehrhard Nicole, Elvert Ralf, Gau Christine, Hölter Sabine M, Micklich Katja, Moreth Kristin, Prehn Cornelia, Puk Oliver, Racz Ildiko, Stoeger Claudia, Vernaleken Alexandra, Michel Dian, Diener Susanne, Wieland Thomas, Adamski Jerzy, Bekeredjian Raffi, Busch Dirk H, Favor John, Graw Jochen, Klingenspor Martin, Lengger Christoph, Maier Holger, Neff Frauke, Ollert Markus, Stoeger Tobias, Yildirim Ali Önder, Strom Tim M, Zimmer Andreas, Wolf Eckhard, Wurst Wolfgang, Klopstock Thomas, Beckers Johannes, Gailus-Durner Valerie, Hrabé de Angelis Martin, ;2016;G3 (Bethesda, Md.);6;4035-4046; 27815347

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C3HeB/FeJ-Scube3m1Mhda/Ieg