C57BL/6N-Atm1Brd Mirc40tm1Hmpr/WtsiOulu

Status

Available to order

EMMA IDEM:10856
International strain nameC57BL/6N-Atm1Brd Mirc40tm1Hmpr/WtsiOulu
Alternative namemiR-96;miR-183 knockout
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolMirc40tm1Hmpr
Gene/Transgene symbolMirc40

Information from provider

ProviderHaydn Prosser
Provider affiliationMouse Genomics/Team 82, Wellcome Trust Sanger Institute
Genetic informationDeletion of miR-96 and miR183 extending chr 6:30169773 to 6:30169423.
Phenotypic informationHomozygous:
Balance and hearing phenotype due to developmental defect in inner ear hair cells.

Heterozygous:
Mild hearing defect.
Breeding historyChimaeras derived from JM8.A3 ES cells (C57BL/6N) were bred with C57BL/6N mice.
References
  • Hearing impairment due to Mir183/96/182 mutations suggests both loss and gain of function effects.;Lewis Morag A, Di Domenico Francesca, Ingham Neil J, Prosser Haydn M, Steel Karen P, ;2020;Disease models & mechanisms;14;; 33318051
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreUniversity of Oulu, Oulu, Finland

Disease and phenotype information

MGI phenotypes (gene matching)
  • abnormal retinal cone cell morphology / MGI
  • abnormal retinal outer nuclear layer morphology / MGI
  • abnormal rod electrophysiology / MGI
  • abnormal cone electrophysiology / MGI
  • abnormal retinal cone cell outer segment morphology / MGI
  • thin retinal outer nuclear layer / MGI
  • abnormal retinal blood vessel morphology / MGI
  • increased microglial cell activation / MGI

Literature references

  • Hearing impairment due to Mir183/96/182 mutations suggests both loss and gain of function effects.;Lewis Morag A, Di Domenico Francesca, Ingham Neil J, Prosser Haydn M, Steel Karen P, ;2020;Disease models & mechanisms;14;; 33318051
  • miR-96 and miR-183 differentially regulate neonatal and adult postinfarct neovascularization.;Castellan Raphael Fp, Vitiello Milena, Vidmar Martina, Johnstone Steven, Iacobazzi Dominga, Mellis David, Cathcart Benjamin, Thomson Adrian, Ruhrberg Christiana, Caputo Massimo, Newby David E, Gray Gillian A, Baker Andrew H, Caporali Andrea, Meloni Marco, ;2020;JCI insight;5;; 32544097

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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